Aligments for a candidate for lpd in Desulfovibrio vulgaris Miyazaki F

GapMind for catabolism of small carbon sources

Aligments for a candidate for lpd in Desulfovibrio vulgaris Miyazaki F

Align Dihydrolipoyl dehydrogenase; EC 1.8.1.4; Dihydrolipoamide dehydrogenase; E3 component of pyruvate and 2-oxoglutarate dehydrogenases complexes (uncharacterized)
to candidate 8499450 DvMF_0222 FAD-dependent pyridine nucleotide-disulphide oxidoreductase (RefSeq)

Query= curated2:Q8KCW2
         (469 letters)



>lcl|FitnessBrowser__Miya:8499450 DvMF_0222 FAD-dependent pyridine
           nucleotide-disulphide oxidoreductase (RefSeq)
          Length = 473

 Score =  279 bits (714), Expect = 1e-79
 Identities = 181/481 (37%), Positives = 259/481 (53%), Gaps = 28/481 (5%)

Query: 6   TLAAQFDVAVIGSGPGGYEAAIHAARYGLKTCIVEKAVLGGVCVNWGCIPTKALLRSAEV 65
           T + ++D+ ++G GPGG  AA  AA  GL   +V++  LGG C+N GCIPTK LL +   
Sbjct: 2   TPSTRYDMIILGGGPGGSRAAFDAAARGLSVALVDRDGLGGTCLNRGCIPTKLLLGATAA 61

Query: 66  FDLAKNPETFGVNVGNVSFDLAQAVKRSRNVALKSSKGVAYLLKKAAVEVLAGEAVLTGG 125
             L +  +      G+++FDL    +R       + + +   LK A + V AGE  +TG 
Sbjct: 62  LPLLETQKKLKGADGHIAFDLPALQQRKDRYVKGTRQALEKRLKAAGIAVYAGEGRVTGE 121

Query: 126 A--------GVMVTMPDGSVR--MLGAKNIIVATGSTPRVIPGLEPDGKKIITSREALIL 175
                     V+    DG+ +   LG   +IVATGSTP   PGL  DG  ++ S   L +
Sbjct: 122 RQGDADGELAVVAAQADGTTQETRLGWGTLIVATGSTPASFPGLAADGAAVLDSTALLDV 181

Query: 176 KEVPKSMIVVGGGAIGVEMAWFYAKAGSKVTIVELMPRMLPAEEAEVSEALKRSFEKAGI 235
            E P+S+IVVGGGAIG+EMA F+++ G+++TIVE M R+ P E+AEV + L++ + + G 
Sbjct: 182 TEAPESLIVVGGGAIGLEMADFFSRFGTRITIVEGMGRLAPTEDAEVGDTLRKVYAREGW 241

Query: 236 TVHCGAKLDNVAVSESGVSAELVVEGSAPQTLNASCLLVAVGVTGAIDGLGLDAVGVETE 295
           T+H G K+ ++A     V    V+     + L AS  L+AVG   A  G+GL+A+G    
Sbjct: 242 TIHNGRKVASLAT----VDGHAVLRFEDGEELTASKALLAVGRRPASVGIGLEALGTTLR 297

Query: 296 -RGFIRTDGQCRTSAPGIYAIGDVRGGMLLAHKASAEAAIAVEAIAGKSPEPLSEPLIPR 354
             G+++TD   R +AP +YAIGDV G  LLAH A  +A  AV    G +  P    ++P 
Sbjct: 298 GPGWMQTDEWLR-AAPHVYAIGDVNGRTLLAHAADHQARYAVRHACGDTAAPYDAGVMPA 356

Query: 355 CVYAQPSVASVGLTEEAAVNAGYQVAVGRSQFAASGKANAYGQLEGFVKLVFDAATGKML 414
           C+Y       VG T E   NAG+   V RS   A+  A AYG  +GF+K+V+    G++ 
Sbjct: 357 CIYGHLEAMRVGPTAEELKNAGFSPRVSRSMLIANPIAQAYGTTQGFIKIVW--VDGRIR 414

Query: 415 GGHLIGHD-------AVELIGELGLACRYGVTAGGLVNTVHAHPTLSETVREAAFDALQS 467
           G   +GH        A  L G  G A   G TA    N + AHPTL E + EAA +A Q 
Sbjct: 415 GVTAVGHGVSHLVTLAAVLAGGSGTAT--GWTAHDAGNVIFAHPTLDEAL-EAAIEAPQE 471

Query: 468 M 468
           +
Sbjct: 472 L 472



 Score = 24.6 bits (52), Expect = 0.007
 Identities = 13/36 (36%), Positives = 18/36 (50%)

Query: 5   DTLAAQFDVAVIGSGPGGYEAAIHAARYGLKTCIVE 40
           D   A   + V+G G  G E A   +R+G +  IVE
Sbjct: 180 DVTEAPESLIVVGGGAIGLEMADFFSRFGTRITIVE 215


Lambda     K      H
   0.316    0.133    0.377 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 635
Number of extensions: 31
Number of successful extensions: 7
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 469
Length of database: 473
Length adjustment: 33
Effective length of query: 436
Effective length of database: 440
Effective search space:   191840
Effective search space used:   191840
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources