GapMind for catabolism of small carbon sources

 

Aligments for a candidate for hisP in Desulfovibrio vulgaris Miyazaki F

Align Amino-acid ABC transporter, ATP-binding protein (characterized, see rationale)
to candidate 8502445 DvMF_3151 ABC transporter related (RefSeq)

Query= uniprot:Q88GX0
         (260 letters)



>lcl|FitnessBrowser__Miya:8502445 DvMF_3151 ABC transporter related
           (RefSeq)
          Length = 242

 Score =  286 bits (733), Expect = 2e-82
 Identities = 138/240 (57%), Positives = 184/240 (76%)

Query: 20  LIRIEGLNKHYGAFHVLRDIDLQVREGERIVLCGPSGSGKSTLIRCINRLEVAQQGSIQV 79
           +I    ++K YG FHVL+ I  +V +GE +V+CGPSGSGKS+ IRC+NRLE  Q+G I +
Sbjct: 3   MIEFHDVHKWYGEFHVLKGITQKVEKGEVLVICGPSGSGKSSFIRCLNRLEPIQKGQILL 62

Query: 80  DGIDLAATTREAAQVRSDIGMVFQHFNLFPHMSVLDNCLLAPTSVRGLSRKDAEERARMY 139
           +G  +     +  ++R+++G+VFQ FNL+PH+SVL N  LAPT VR + +  AE  A   
Sbjct: 63  EGKSIHDKGVDVNELRTEVGIVFQQFNLYPHLSVLHNVTLAPTKVRKMPKAKAESIAMEL 122

Query: 140 LSKVGIESQAHKYPSQLSGGQQQRVAIARALCMKPRIMLFDEPTSALDPEMVAEVLDVLV 199
           L +VGI  QA KYP +LSGGQQQRVAIARAL M+P++MLFDEPTSALDPEM+ EVL+ + 
Sbjct: 123 LERVGIHDQARKYPVELSGGQQQRVAIARALAMQPKVMLFDEPTSALDPEMINEVLNAMK 182

Query: 200 QLAGTGMTMLCVTHEMGFARQVAERVLFLEGGQIIEDSPPQVFFNQPRTERAKAFLAQIL 259
            LA  GMTMLCVTHEMGFAR+VA+RV+F++GG++IE++PP VFF+ P+ ER +AFL +IL
Sbjct: 183 DLARAGMTMLCVTHEMGFAREVADRVVFMDGGKVIEEAPPDVFFSSPKHERTQAFLREIL 242


Lambda     K      H
   0.322    0.137    0.395 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 204
Number of extensions: 6
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 260
Length of database: 242
Length adjustment: 24
Effective length of query: 236
Effective length of database: 218
Effective search space:    51448
Effective search space used:    51448
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 46 (22.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory