Align Phenylacetyl-CoA:acceptor oxidoreductase small subunit PadC (characterized, see rationale)
to candidate 8499289 DvMF_0067 4Fe-4S ferredoxin iron-sulfur binding domain protein (RefSeq)
Query= uniprot:A0A2R4BLY8 (215 letters) >FitnessBrowser__Miya:8499289 Length = 259 Score = 136 bits (342), Expect = 4e-37 Identities = 83/226 (36%), Positives = 109/226 (48%), Gaps = 35/226 (15%) Query: 3 RYAMVADLRRCVG---CQTCTAACKHTNATPP-----GVQWRWVLDVEAGEFPDVSRTFV 54 R+AMV D R+ + AC + P ++W W + D+S+ Sbjct: 51 RWAMVIDTRKLESREDFERIIHACHSVHNVPSIPSKQEIKWIWTDKYDRVFTDDMSQHLS 110 Query: 55 PVG--------CQHCDEPPCETVCPTTATKKRADGLVTIDYDLCIGCAYCSVACPYNARY 106 P C HC+ PPC VCPT AT KRADG+V +DY CIGC +C CPY AR Sbjct: 111 PAVREKDYLLLCNHCENPPCVRVCPTKATFKRADGIVVMDYHRCIGCRFCMAGCPYGAR- 169 Query: 107 KVNFAEPAYGDRLMANEKQRADPARV-GVATKCTFCSDRIDYGVAHGLTPGVDPDATPAC 165 NF +P R + A P R+ GV KCTFCS+R++ G+ PAC Sbjct: 170 SFNFGDP----RPYLKDVNPAFPTRMRGVVEKCTFCSERLEVGL------------LPAC 213 Query: 166 ANACIANALTFGDIDDPNSKASRLLRENEHFRMHEELGTGPGFFYL 211 A A+ FGD+DDPNS + L EN R +GT PG +Y+ Sbjct: 214 VEAS-NGAILFGDLDDPNSPVRKALAENFSIRRKPSIGTQPGVYYI 258 Lambda K H 0.323 0.137 0.461 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 240 Number of extensions: 17 Number of successful extensions: 4 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 215 Length of database: 259 Length adjustment: 23 Effective length of query: 192 Effective length of database: 236 Effective search space: 45312 Effective search space used: 45312 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 46 (22.3 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory