Align L-lactate permease (characterized, see rationale)
to candidate 8501398 DvMF_2129 L-lactate transport (RefSeq)
Query= uniprot:Q8EIL2 (545 letters) >FitnessBrowser__Miya:8501398 Length = 551 Score = 574 bits (1480), Expect = e-168 Identities = 290/550 (52%), Positives = 385/550 (70%), Gaps = 10/550 (1%) Query: 1 MTWTQTYTPLGSLWLTAIVALLPIVFFFLALTVLKLKGHIAGALTLLIALAVAIITYKMP 60 M W Q Y PL ++ L+A+VA +P+ F L V + KGH A AL A+ +AI+ + MP Sbjct: 1 MNWVQNYDPLSNILLSALVAAVPLYVLFYMLAVRRAKGHFAAALGTTAAVLLAILVWGMP 60 Query: 61 VSIALASAIYGFSYGLWPIAWIIITAVFLYKITVKTGQFEIIRSSVISVTEDQRLQMLLV 120 V++A+ + +YG +YGL+PI WI+ITAV++Y +TV++G+FEII+ S+ +T+D+RLQ + + Sbjct: 61 VNLAVNATLYGAAYGLFPIVWIVITAVWIYNMTVESGEFEIIKDSLARLTDDRRLQAIFI 120 Query: 121 GFSFGAFLEGAAGFGAPVAITAALLVGLGFNPLYAAGLCLIANTAPVAFGAMGIPIIVAG 180 F+FG+F+EG AGFG PVAITAA+LVGLGFNPLYAAG+CLIANTAPVAFGA+GIP+IVAG Sbjct: 121 AFAFGSFIEGTAGFGTPVAITAAMLVGLGFNPLYAAGICLIANTAPVAFGAIGIPVIVAG 180 Query: 181 QVSSLDPFHIGQLAGRQLPILSIIVPFWLIAMMDGIRGIRQTWPATLVAGVSFAVTQFLT 240 QVS LD HI Q+ GRQLP LSIIVP WL M G + + PA +VAGV FA +Q+ Sbjct: 181 QVSGLDTMHISQIVGRQLPFLSIIVPLWLCVTMCGFKRSLEVLPAVIVAGVCFAGSQYAF 240 Query: 241 SNFIGPELPDITSALVSLICLTLFLKVWQPKEIFTFSGMKQRAVTPKSTFSNGQIFKAWS 300 SNF GP LPDI SA++++I L L L+VW+P + F ++T S +S G+I +AW Sbjct: 241 SNFHGPTLPDIMSAIITIIGLVLLLRVWKPATTWHFPDETPSSLTGPSPYSFGEIIRAWM 300 Query: 301 PFIILTAIVTLWSIKDVQ------LALSFATISIE---VPYLHNLVIKTAPIVAKETPYA 351 P+IIL +V W + +++ L F ++ + P LHN + KTAPIVA PY Sbjct: 301 PYIILAIMVFFWGLPEIKPYLDGALGAGFTPLAAKGFAWPGLHNAIEKTAPIVAANAPYG 360 Query: 352 AIYKLNLLGAVGTAILIAAMISIVVL-KMSISNALTSFKDTLIELRFPILSIGLVLAFAF 410 A++KLN++ A GTAIL + ++ ++ K A+ T+ +LRFPIL+I ++L A Sbjct: 361 AMFKLNIISAAGTAILFTGLAAVPMMPKYGYGQAIPCLFRTMHQLRFPILTIAMILGLAQ 420 Query: 411 VANYSGLSSTLALVLAGTGVAFPFFSPFLGWLGVFLTGSDTSSNALFGALQANTANQIGV 470 + NYSG+SST+ L TG FPFFSP LGWLGVFLTGSDTSSNALF LQ TA +G+ Sbjct: 421 IMNYSGMSSTMGLAFTKTGWLFPFFSPLLGWLGVFLTGSDTSSNALFSGLQRTTATSVGM 480 Query: 471 TPELLVAANTTGGVTGKMISPQSIAVACAATGLAGKESDLFRFTLKHSLFFCTFIGVLTV 530 P L VAAN++GGVTGKMISPQSI+VA AAT + G+E LFRFTL HS+ FI VLT+ Sbjct: 481 DPHLAVAANSSGGVTGKMISPQSISVATAATKMVGQEGQLFRFTLWHSVAMTAFICVLTM 540 Query: 531 LQAYIVPWTL 540 LQAY + W L Sbjct: 541 LQAYPLKWML 550 Lambda K H 0.327 0.140 0.419 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 977 Number of extensions: 32 Number of successful extensions: 4 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 545 Length of database: 551 Length adjustment: 36 Effective length of query: 509 Effective length of database: 515 Effective search space: 262135 Effective search space used: 262135 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 53 (25.0 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory