Alignments for a candidate for prpE in Desulfovibrio vulgaris Miyazaki F

GapMind for catabolism of small carbon sources

Alignments for a candidate for prpE in Desulfovibrio vulgaris Miyazaki F

Align Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; Acetate--CoA ligase; Acyl-activating enzyme; EC 6.2.1.1 (characterized)
to candidate 8502070 DvMF_2783 acetate/CoA ligase (RefSeq)

Query= SwissProt::P31638
         (660 letters)



>FitnessBrowser__Miya:8502070
          Length = 651

 Score =  585 bits (1507), Expect = e-171
 Identities = 303/652 (46%), Positives = 423/652 (64%), Gaps = 17/652 (2%)

Query: 4   IESVMQEHRVFNPPEGFASQAAIPSMEAYQALCDEAERDYEGFWARHARELLHWTKPFTK 63
           I+++++E RVF P     + A +   +  +A    A  D +G+W   A EL  W + +  
Sbjct: 8   IDALLREERVFRPLPQVVAGAVVNPQDVARARA-RAAADPDGYWEEAAEEL-EWFRRWDA 65

Query: 64  VLDQSNAPFYKWFEDGELNASYNCLDRNLQNGNADKVAIVFEADDGSVTRVTYRELHGKV 123
           V D SNAPF++WF     N  +N LDR+++ G  +++A+++E + G     TY +L+ +V
Sbjct: 66  VHDGSNAPFHRWFTGARCNIVHNALDRHIETGTKNRLALIWEGESGDTRSFTYYQLYREV 125

Query: 124 CRFANGLKALGIRKGDRVVIYMPMSVEGVVAMQACARLGATHSVVFGGFSAKSLQERLVD 183
            R AN L+ LG+ KGDRV+IYMP   E V AM A A++GA HS VFGGFSA+SL++R+ D
Sbjct: 126 NRLANALRGLGVGKGDRVIIYMPPLPETVFAMLAAAKIGAVHSTVFGGFSARSLRDRMED 185

Query: 184 VGAVALITADEQMRGGKALPLKAIADDALAL---GGCEAVRNVIVYRRTGGKVAWTEGRD 240
                ++T D   R G+ +PLK IAD+A+A         VR+++V  R   +   TEGRD
Sbjct: 186 ARPAVIVTVDGFYRNGRVIPLKPIADEAVATLPPDLAAGVRHMVVVHRAHVETPMTEGRD 245

Query: 241 RWMEDVSAGQPDTCEAEPVSAEHPLFVLYTSGSTGKPKGVQHSTGGYLLWALMTMKWTFD 300
            W  D   GQ      E + +  PLF+LYTSG+TGKPKG  H+ GGY++    TM+W FD
Sbjct: 246 IWYHDAVRGQHHEALTEIMDSTDPLFLLYTSGTTGKPKGHVHAHGGYMVGVHRTMRWVFD 305

Query: 301 IKPDDLFWCTADIGWVTGHTYIAYGPLAAGATQVVFEGVPTYPNAGRFWDMIARHKVSIF 360
           +KP D+FWCTA+ GW+TGH+Y+ YGPL AG T V++EG P YP  GR W M+ R  ++I 
Sbjct: 306 VKPTDIFWCTAEPGWITGHSYVVYGPLMAGTTTVLYEGHPLYPEPGRVWSMVERLGITIL 365

Query: 361 YTAPTAIRSLIKAAEADEKIHPKQYDLSSLRLLGTVGEPINPEAWMWYYKNIGNERCPIV 420
           YTAPT +R L++        H  ++DL++LRLLGTVGEPI+PEAW+W+++++G  RCP++
Sbjct: 366 YTAPTLVRMLMRHGAQ----HVARHDLTTLRLLGTVGEPISPEAWLWFHRHVGRGRCPVL 421

Query: 421 DTFWQTETGGHMITPLPGATPLVPGSCTLPLPGIMAAIVDETGHDVPNGNGGILVVKRPW 480
           DT+WQTETG  M++PLP  + L PGS T PLPGI A +VDE G  V  G+GG+LV++RPW
Sbjct: 422 DTWWQTETGMIMLSPLP-VSLLKPGSVTRPLPGIEADVVDEHGKPVGPGHGGLLVLQRPW 480

Query: 481 PAMIRTIWGDPERFRKSYFPEELGGKLYLAGDGSIRDKDTGYFTIMGRIDDVLNVSGHRM 540
           PAM   ++ D E +R+ Y+    G   Y  GD + RD+D GYF I GR DDVL ++GHR+
Sbjct: 481 PAMSCGVYNDDESYRRLYWERFPG--WYCTGDVARRDED-GYFWIQGRADDVLLIAGHRI 537

Query: 541 GTMEIESALVSNPLVAEAAVVGRPDDMTGEAICAFVVLKRSRP---TGEEAVKIATELRN 597
           GT E+E+AL S+  VAE AV+G PD + GE   AFVVL    P   T   A ++A EL  
Sbjct: 538 GTAEMEAALASHRSVAECAVIGVPDALRGEVAKAFVVLVDDHPPLGTMVNADELAAELVE 597

Query: 598 WVGKEIGPIAKPKDIRFGDNLPKTRSGKIMRRLLRSLAKGEEITQDTSTLEN 649
            V +E+GP+A  +++ F + LP+ RSGKIMRR+LRS   G + T D STLE+
Sbjct: 598 HVRRELGPVAVIREVSFREGLPRNRSGKIMRRVLRSEELGRD-TGDLSTLED 648


Lambda     K      H
   0.319    0.136    0.422 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1349
Number of extensions: 74
Number of successful extensions: 8
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 660
Length of database: 651
Length adjustment: 38
Effective length of query: 622
Effective length of database: 613
Effective search space:   381286
Effective search space used:   381286
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 54 (25.4 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources