Aligments for a candidate for potA in Desulfovibrio vulgaris Miyazaki F

GapMind for catabolism of small carbon sources

Aligments for a candidate for potA in Desulfovibrio vulgaris Miyazaki F

Align PotG aka B0855, component of Putrescine porter (characterized)
to candidate 8502259 DvMF_2969 ABC transporter related (RefSeq)

Query= TCDB::P31134
         (377 letters)



>FitnessBrowser__Miya:8502259
          Length = 418

 Score =  248 bits (634), Expect = 2e-70
 Identities = 130/313 (41%), Positives = 193/313 (61%), Gaps = 5/313 (1%)

Query: 2   NDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYKGEIFALLGASGCGKSTL 61
           +DA P        + +  L +  L K ++   AV+DVS  + +G  F++LG SGCGK+TL
Sbjct: 11  SDAFPNASPVASPSASADLSVTRLVKRFEKFTAVNDVSFEVEQGRFFSILGPSGCGKTTL 70

Query: 62  LRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYALFPHMTVEQNIAFGLKQDKL 121
           LRM+AGFE P +G I + G D++ + P  RP+N++FQ  ALFP M+V +N+AFGLK+  +
Sbjct: 71  LRMIAGFESPDSGVIAIRGRDMAGIAPNRRPVNLIFQHLALFPMMSVAENVAFGLKRRGM 130

Query: 122 PKAEIASRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVALARSLAKRPKLLLLDEPMGALD 181
              EI+ RV ++L  V +  +  + P QLSGGQ+QRVA+AR L   P +LLLDEP+GALD
Sbjct: 131 AGGEISRRVQDVLERVGLPGYGVKMPAQLSGGQKQRVAIARCLVLEPAVLLLDEPLGALD 190

Query: 182 KKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAGRIAIMNRGKFVQIGEPEEIYEHP 241
            KLR++M++E+  +   VG T V +THDQ EA+ M+  +A+MN G+F Q+  P  +Y  P
Sbjct: 191 LKLREQMKVELKTLQAEVGTTFVYITHDQSEALVMSDHVAVMNAGRFEQVDTPRNLYRRP 250

Query: 242 TTRYSAEFIGSVNVFEGVLKERQED-GLVLDSPGLVHPLKVDADASVVDNVPVHVALRPE 300
            + + A F+G  NV+ G L+E   D GLV    G V   +V   A +     V + +RPE
Sbjct: 251 ASAFVAGFVGETNVWSGTLEEATGDAGLVRTDEGAVFRARV--AAGLAKGTRVDMFIRPE 308

Query: 301 KIMLCEEPPANGC 313
            +++  +P    C
Sbjct: 309 AVLI--DPDGASC 319


Lambda     K      H
   0.321    0.137    0.400 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 361
Number of extensions: 14
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 377
Length of database: 418
Length adjustment: 31
Effective length of query: 346
Effective length of database: 387
Effective search space:   133902
Effective search space used:   133902
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 50 (23.9 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources