Alignments for a candidate for rbsA in Desulfovibrio vulgaris Miyazaki F

GapMind for catabolism of small carbon sources

Alignments for a candidate for rbsA in Desulfovibrio vulgaris Miyazaki F

Align ribose transport, ATP-binding protein RbsA; EC 3.6.3.17 (characterized)
to candidate 8502321 DvMF_3029 ABC transporter related (RefSeq)

Query= CharProtDB::CH_003578
         (501 letters)



>FitnessBrowser__Miya:8502321
          Length = 537

 Score =  268 bits (685), Expect = 4e-76
 Identities = 158/484 (32%), Positives = 268/484 (55%), Gaps = 10/484 (2%)

Query: 4   LLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKSTMMKVLTGIYTRDAGTLLW 63
           +++L GI K+F  V+A     L++ PG + AL+GENGAGKST+M +L G   +DAGT++ 
Sbjct: 29  VVRLDGICKSFGKVRANHDITLDIRPGCIKALLGENGAGKSTLMSILAGKLRQDAGTIVV 88

Query: 64  LGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFLGR--EFVNRFGKIDWKTMYAE 121
            G  T F  P+ +  AGIG+++Q   L+  +T+AEN+ LG+  + + R  +     M  E
Sbjct: 89  DGVPTVFASPRDALRAGIGMVYQHFMLVDSMTVAENVLLGQSPDMLLRPAR-----MRDE 143

Query: 122 ADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFESKVIIMDEPTDALTDTETESLF 181
              L  +  L       VG LS+G++Q VEI K+L  +S+V+I+DEPT  LT  ET+ LF
Sbjct: 144 VAALAERYGLAVDPAARVGGLSMGERQRVEILKLLYRDSRVLILDEPTAVLTPRETDQLF 203

Query: 182 RVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQFIAEREVASLTEDS-LIEMMVGR 240
             +  +  QG+ +V+ISH+++E+  + D++ + R G+ + E   A +   + L   MVGR
Sbjct: 204 EAMWRMADQGKALVFISHKLQEVLTVADEIAILRRGEVVDEFSEADVPNQTVLANRMVGR 263

Query: 241 KLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLRKGEILGVSGLMGAGRTELMKVLYG 300
            +  Q       P D  L V++L G G++DVS  +R+GEI+ ++G+ G G+ EL++ + G
Sbjct: 264 DVVLQVDAKRLTPVDTVLSVEHLSGAGLSDVSLQVRRGEIVAIAGVAGNGQKELVEAICG 323

Query: 301 ALPRTSGYVTLDGHEVVTRSPQDGLANGIVYISEDRKRDGLVLGMSVKENMSLTALRYFS 360
                +G V + G              G+ YI EDR+       + + +N  LT    F+
Sbjct: 324 LARPEAGEVRILGRPWREFFAGPPGRRGLAYIPEDRQGLATCRHLDLVDNFLLTTRNQFA 383

Query: 361 RAGGSLKHADEQQAVSDFIRLFNVKTPSMEQAIGLLSGGNQQKVAIARGLMTRPKVLILD 420
           + G  L   +   AV   +  +NV+   +      LSGGN QK+ I R    +P+V++ +
Sbjct: 384 K-GVFLDRTEATNAVKRVVWEYNVQPGDITAPARALSGGNLQKLVIGREFFRKPEVIVAE 442

Query: 421 EPTRGVDVGAKKEIYQLINQFKADGLSIILVSSEMPEVLGMSDRIIVMHEGHLSGEFTRE 480
            PT+G+D+ A +E++  + + ++    ++LV+ ++ E L ++DRI VM+ G     F ++
Sbjct: 443 NPTQGLDISATEEVWGRLLEARSTS-GVLLVTGDLNEALELADRIAVMYRGRFIDVFDKD 501

Query: 481 QATQ 484
              +
Sbjct: 502 DTAK 505



 Score = 85.9 bits (211), Expect = 3e-21
 Identities = 68/255 (26%), Positives = 124/255 (48%), Gaps = 19/255 (7%)

Query: 240 RKLEDQYPHLDKAPGDIRLKVDNLCGP-----GVNDVSFTLRKGEILGVSGLMGAGRTEL 294
           R++ + + HL   P  +RL  D +C         +D++  +R G I  + G  GAG++ L
Sbjct: 17  RRVRETHAHL---PPVVRL--DGICKSFGKVRANHDITLDIRPGCIKALLGENGAGKSTL 71

Query: 295 MKVLYGALPRTSGYVTLDGHEVVTRSPQDGLANGIVYISEDRKRDGLVLGMSVKENMSLT 354
           M +L G L + +G + +DG   V  SP+D L  GI  + +      LV  M+V EN+ L 
Sbjct: 72  MSILAGKLRQDAGTIVVDGVPTVFASPRDALRAGIGMVYQHFM---LVDSMTVAENVLLG 128

Query: 355 ALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQAIGLLSGGNQQKVAIARGLMTRP 414
                      L+ A  +  V+     + +      + +G LS G +Q+V I + L    
Sbjct: 129 -----QSPDMLLRPARMRDEVAALAERYGLAVDPAAR-VGGLSMGERQRVEILKLLYRDS 182

Query: 415 KVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIILVSSEMPEVLGMSDRIIVMHEGHLS 474
           +VLILDEPT  +      ++++ + +    G +++ +S ++ EVL ++D I ++  G + 
Sbjct: 183 RVLILDEPTAVLTPRETDQLFEAMWRMADQGKALVFISHKLQEVLTVADEIAILRRGEVV 242

Query: 475 GEFTREQATQEVLMA 489
            EF+      + ++A
Sbjct: 243 DEFSEADVPNQTVLA 257


Lambda     K      H
   0.318    0.137    0.380 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 646
Number of extensions: 28
Number of successful extensions: 10
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 501
Length of database: 537
Length adjustment: 35
Effective length of query: 466
Effective length of database: 502
Effective search space:   233932
Effective search space used:   233932
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources