Alignments for a candidate for dauA in Desulfovibrio vulgaris Miyazaki F

GapMind for catabolism of small carbon sources

Alignments for a candidate for dauA in Desulfovibrio vulgaris Miyazaki F

Align C4-dicarboxylic acid transporter DauA; Dicarboxylic acid uptake system A (characterized)
to candidate 8500834 DvMF_1575 sulfate transporter (RefSeq)

Query= SwissProt::P0AFR2
         (559 letters)



>FitnessBrowser__Miya:8500834
          Length = 570

 Score =  385 bits (990), Expect = e-111
 Identities = 228/544 (41%), Positives = 322/544 (59%), Gaps = 39/544 (7%)

Query: 24  YTAARFTRDLIAGITVGIIAIPLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVS 83
           Y+  +F RDL+AG+TVG++A+PLAMA AI SG  P+ GL+TA VAG +I+L GGSR+ + 
Sbjct: 26  YSTQKFIRDLLAGLTVGVVALPLAMAFAIASGTTPERGLFTAIVAGFLISLLGGSRYQIG 85

Query: 84  GPTAAFVVILYPVSQQFGLAGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGI 143
           GPT AFVVI+Y V  + G  GL++ TLL+G  L++ GL R G LI+YIP  VT GFT+GI
Sbjct: 86  GPTGAFVVIIYNVIYRHGYDGLVITTLLAGAMLLIFGLCRIGVLIKYIPYPVTTGFTAGI 145

Query: 144 GITIGTMQIKDFLGLQMAHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLG 203
            + I + Q+KDF GLQM  VP  +  K  A    L TIN     +  + L  ++   R  
Sbjct: 146 AVLIFSSQMKDFFGLQMDAVPPEFFDKWLAYAEHLGTINTTTLCVAGLALACILATRRF- 204

Query: 204 IRLPGHLPALLAGCAVMGI-VNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWD 262
             +P  +PA + G AV  + V  LG  V TIGS+F           GIP+ LP  V P  
Sbjct: 205 --IP-RIPAPVVGVAVASLAVWALGLQVETIGSRF----------GGIPRELPSFVWP-- 249

Query: 263 LPNSEFTLTWDSIRTLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNI 322
                 T T+  +R LLP A ++A+L  IESLL  VV DGMTG KH +N EL  QG+ NI
Sbjct: 250 ------TFTFARVRELLPDAMTIALLAGIESLLSCVVADGMTGDKHNSNVELTAQGVANI 303

Query: 323 IAPFFGGITATAAIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAAL 382
            +  FGGI AT AIAR+  N+R+G  +P++ ++H+ +++  +L LAPL S++PL+++AA+
Sbjct: 304 ASVLFGGIPATGAIARTVTNIRSGGQTPVAGMVHAAVLVAFVLFLAPLASFIPLASLAAV 363

Query: 383 LLMVAWNMSEAHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRI 442
           L+MV+W+MSE HK + LLR APK DI VM L   LTV+ D+ +A+ VG++LASLLFMRR+
Sbjct: 364 LIMVSWDMSELHKFLRLLR-APKSDITVMCLTFVLTVVIDLTVAVYVGVMLASLLFMRRM 422

Query: 443 ARMTRLAPVVVD--------------VPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKR 488
           + +T +    +D              VP+ V V  + GP FF  A+   +  ++  +   
Sbjct: 423 SEVTAICSCAIDDKPVIQGRETAELVVPEGVQVYEIDGPFFFGVADRFQSVSQALQKQPE 482

Query: 489 IVILKWDAVPVLDAGGLDAFQRFVKRL-PEGCELRVCNVEFQPLRTMARAGIQPIPGRLA 547
           + IL+      +D+ G +A + F +     G  L +  V     R M R G   + GR  
Sbjct: 483 VFILRMRKASTVDSTGANALEAFCRTCRRRGTVLLLSGVRPAARRMMERFGTLDLIGREN 542

Query: 548 FFPN 551
            F N
Sbjct: 543 LFEN 546


Lambda     K      H
   0.328    0.142    0.425 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 668
Number of extensions: 36
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 559
Length of database: 570
Length adjustment: 36
Effective length of query: 523
Effective length of database: 534
Effective search space:   279282
Effective search space used:   279282
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
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Protein sequences (fasta format)
Organisms (tab-delimited)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources