GapMind for catabolism of small carbon sources

 

Alignments for a candidate for thuK in Desulfovibrio vulgaris Miyazaki F

Align Sugar-binding transport ATP-binding protein aka MalK1 aka TT_C0211, component of The trehalose/maltose/sucrose/palatinose porter (TTC1627-9) plus MalK1 (ABC protein, shared with 3.A.1.1.24) (Silva et al. 2005; Chevance et al., 2006). The receptor (TTC1627) binds disaccharide alpha-glycosides, namely trehalose (alpha-1,1), sucrose (alpha-1,2), maltose (alpha-1,4), palatinose (alpha-1,6) and glucose (characterized)
to candidate 8500851 DvMF_1589 ABC transporter related (RefSeq)

Query= TCDB::Q72L52
         (376 letters)



>FitnessBrowser__Miya:8500851
          Length = 354

 Score =  254 bits (648), Expect = 3e-72
 Identities = 155/344 (45%), Positives = 203/344 (59%), Gaps = 18/344 (5%)

Query: 1   MAKVRLEHVWKRFGKVVAVKDFNLETEDGEFVVFVGPSGCGKTTTLRMIAGLEEISEGNI 60
           M+ VRL +V KRFG V AV   NLE   GE    +GPSGCGKTTTLRM+AG E++ +G I
Sbjct: 1   MSYVRLVNVTKRFGGVTAVDSLNLEIGRGECFSMLGPSGCGKTTTLRMVAGFEDLDDGEI 60

Query: 61  YIGDRLVND------VPPKDRDIAMVFQNYALYPHMNVYENMAFGLRLRRYPKDEIDRRV 114
           ++GDRL++       +PP+ RD  MVFQ +A++PH++VYEN+AF LR+RR    EIDRR 
Sbjct: 61  HVGDRLLSARRNNYYLPPEKRDFGMVFQAFAVWPHLSVYENVAFPLRIRRLSAAEIDRRT 120

Query: 115 KEAARILKIEHLLNRKPRELSGGQRQRVAMGRAIVREPKVFLMDEPLSNLDAKLRVEMRA 174
           +EA     +  +  + P +LSGG +QRVA+ RA+   P V L+DEPLS+LD  LR EMR 
Sbjct: 121 REALHHTSLADVAQKSPDDLSGGGKQRVALARALAINPDVMLLDEPLSSLDPHLREEMRF 180

Query: 175 EIAKLQRRLGVTTIYVTHDQVEAMTLGHRIVVMKDGEIQQVDTPLNLYDFPANRFVAGFI 234
           EI  LQR  G + +YVTHDQ EAM L  RI+VM++G +QQV TPL++Y  PAN FV GFI
Sbjct: 181 EIKDLQRTFGFSILYVTHDQSEAMALSDRIMVMRNGVVQQVGTPLDVYTNPANSFVFGFI 240

Query: 235 GSPSMNFVRAGVEVQGEKVYLVAPGFRIRANAVLGSALKPYAGKEVWLGVRPEHLGLKGY 294
           G    NF+   V +  E +  V  G      A   SA    AG+   L  RP  +     
Sbjct: 241 G--LSNFL--DVNLTPEGLVRVNGGDARVTPATPPSARLVSAGRAA-LASRPSEIDFTA- 294

Query: 295 TTIPEEENVLRGEVEVVEPLGAETEIHVAVNGTLLVAKVDGHAP 338
                 E  LRG V     LG   +  + V+G  +  +    AP
Sbjct: 295 ------EGGLRGVVRRRAYLGEIVDYRIDVSGQEVRVQKGRRAP 332


Lambda     K      H
   0.320    0.139    0.400 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 337
Number of extensions: 13
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 376
Length of database: 354
Length adjustment: 30
Effective length of query: 346
Effective length of database: 324
Effective search space:   112104
Effective search space used:   112104
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 49 (23.5 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory