GapMind for catabolism of small carbon sources

 

Alignments for a candidate for braF in Desulfovibrio vulgaris Miyazaki F

Align High-affinity branched-chain amino acid transport ATP-binding protein BraF, component of Branched chain amino acid uptake transporter. Transports alanine (characterized)
to candidate 8501895 DvMF_2610 ABC transporter related (RefSeq)

Query= TCDB::P21629
         (255 letters)



>FitnessBrowser__Miya:8501895
          Length = 270

 Score =  244 bits (622), Expect = 2e-69
 Identities = 121/255 (47%), Positives = 171/255 (67%), Gaps = 5/255 (1%)

Query: 5   ILEVSGLTMRFGGLLAVNGVNLKVEEKQVVSMIGPNGAGKTTVFNCLTGFYQPTGGLIRL 64
           +L+V  ++M FGGL A+N V+L+V + ++V++IGPNGAGKTT FNC+TG Y PT G + +
Sbjct: 12  VLDVRSISMNFGGLRALNEVDLQVRQGEIVALIGPNGAGKTTFFNCITGIYVPTEGTVHV 71

Query: 65  ---DGEEI--QGLPGHKIARKGVVRTFQNVRLFKEMTAVENLLVAQHRHLNTNFLAGLFK 119
              DG  +   GL   ++   G+ RTFQN+RLF  M+ +EN+++ +H       L  L +
Sbjct: 72  TPRDGRTVTVNGLKASQVTALGMSRTFQNIRLFPTMSVLENVMIGRHCRTRAGILGALLR 131

Query: 120 TPAFRRSEREAMEYAAHWLEEVNLTEFANRSAGTLAYGQQRRLEIARCMMTRPRILMLDE 179
            P  R  E+  +E +   L+ VNL +     A  L YG QRRLEIAR + T P +L LDE
Sbjct: 132 DPKTRAEEQRIVEESYALLKSVNLHQHYKDEARNLPYGAQRRLEIARALATEPFLLCLDE 191

Query: 180 PAAGLNPKETDDLKALIAKLRSEHNVTVLLIEHDMKLVMSISDHIVVINQGAPLADGTPE 239
           PAAG+NP+ET +LK L+ ++R  H ++VLLIEHDM +VMS+SD I V+  G+ +A+GTPE
Sbjct: 192 PAAGMNPQETHELKELVIEIRERHELSVLLIEHDMSMVMSLSDRIYVMEYGSKIAEGTPE 251

Query: 240 QIRDNPDVIKAYLGE 254
           ++  NP VIKAYLGE
Sbjct: 252 EVSKNPRVIKAYLGE 266


Lambda     K      H
   0.320    0.137    0.391 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 198
Number of extensions: 7
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 255
Length of database: 270
Length adjustment: 25
Effective length of query: 230
Effective length of database: 245
Effective search space:    56350
Effective search space used:    56350
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 47 (22.7 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory