GapMind for catabolism of small carbon sources

 

Aligments for a candidate for gcvP in Desulfovibrio vulgaris Miyazaki F

Align Probable glycine dehydrogenase (decarboxylating) subunit 1; EC 1.4.4.2; Glycine cleavage system P-protein subunit 1; Glycine decarboxylase subunit 1; Glycine dehydrogenase (aminomethyl-transferring) subunit 1 (uncharacterized)
to candidate 8499452 DvMF_0224 glycine dehydrogenase subunit 1 (RefSeq)

Query= curated2:Q311A7
         (443 letters)



>lcl|FitnessBrowser__Miya:8499452 DvMF_0224 glycine dehydrogenase
           subunit 1 (RefSeq)
          Length = 443

 Score =  692 bits (1787), Expect = 0.0
 Identities = 336/443 (75%), Positives = 391/443 (88%)

Query: 1   MPYTPHTPEEIRQMLDVIGVKSIDDLFAEIPPEMQPRSFDIPEGMSEMEVCARIQRMAAK 60
           MP+ PH+PEE+R+ML VIGV+SI+DLF +IP EM+PRSF++P G+SEM+V A+++ MAA+
Sbjct: 1   MPFIPHSPEEVREMLSVIGVQSIEDLFVDIPAEMRPRSFELPLGLSEMQVLAKMEEMAAR 60

Query: 61  NRIDLVSYLGAGFYDHHIPKAVDNLVSRGEFYTAYTPYQPEASQGTLQAIFEYQTAVCRL 120
           NR D+VS+LG GFY HHIP AVD LVSRGEFYTAYTPYQPEASQGTLQAIFEYQTAVCRL
Sbjct: 61  NRTDVVSFLGGGFYSHHIPAAVDALVSRGEFYTAYTPYQPEASQGTLQAIFEYQTAVCRL 120

Query: 121 MEMEVANASVYDGGSAIFEAMMMAARATRRSKLVIDEALSPIYRTMLASYTSNLNMELVT 180
           ++M+ ANASVYDGGSAIFEAMMMA RAT+R KLVIDEA+SPI+RTMLASYTSNL+++LVT
Sbjct: 121 LDMDCANASVYDGGSAIFEAMMMAVRATKRRKLVIDEAVSPIWRTMLASYTSNLSLDLVT 180

Query: 181 VAHNEGRSDKQALMDAVDDKCAAVVVQNPNFFGAVDDFTELFTHARSCNALGVISVYPVM 240
           V   +GRSD  A+  AVD  CAAVVVQNPNFFG V+DFT+LF HA+S  A  VISVYPVM
Sbjct: 181 VPQVDGRSDMAAMKAAVDTGCAAVVVQNPNFFGVVEDFTDLFAHAKSQKAASVISVYPVM 240

Query: 241 QSVLKTPGEMGADIAVADGQSLGMPLSFGGPYLGIMTCTKKLARQIPGRIAGRTKDVDGK 300
           QSVLKTPGEMGADIAVA+GQSLG PLSFGGPYLGIMTCTK + RQ+PGRI GRT D +G+
Sbjct: 241 QSVLKTPGEMGADIAVAEGQSLGQPLSFGGPYLGIMTCTKDMVRQMPGRIVGRTNDTEGR 300

Query: 301 TGYVLTLQAREQHIRRAKATSNICSNQALCALRAIIHMCLTGPEGLVRTAELSMERAHYA 360
           TGYVLTLQAREQHIRRAKATSNICSNQALCALR ++H+CL GPEGL+RTAELSMERA YA
Sbjct: 301 TGYVLTLQAREQHIRRAKATSNICSNQALCALRTLVHLCLLGPEGLIRTAELSMERARYA 360

Query: 361 ADRLTALPGVSLLHDAPFCNEFALRLPVSAYDVVDRLVNHGVVPGFPLGGYYAGMDDVLL 420
            +RLTA+ GV  L+ APF NEFA+RLP+ A++ VDRL   G VPGFP+G YYAGMDDVLL
Sbjct: 361 MERLTAIAGVRPLNTAPFGNEFAVRLPIPAFEAVDRLTARGYVPGFPVGRYYAGMDDVLL 420

Query: 421 VACTEKHSFEQIGIMAELVGGML 443
           VACTEK+SFEQ+GI+AE++GG+L
Sbjct: 421 VACTEKNSFEQVGILAEMLGGIL 443


Lambda     K      H
   0.321    0.136    0.398 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 672
Number of extensions: 15
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 443
Length of database: 443
Length adjustment: 32
Effective length of query: 411
Effective length of database: 411
Effective search space:   168921
Effective search space used:   168921
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory