GapMind for catabolism of small carbon sources

 

Aligments for a candidate for tdh in Desulfovibrio vulgaris Miyazaki F

Align L-threonine dehydrogenase (EC 1.1.1.103) (characterized)
to candidate 8499551 DvMF_0321 iron-containing alcohol dehydrogenase (RefSeq)

Query= ecocyc::EG12293-MONOMER
         (383 letters)



>lcl|FitnessBrowser__Miya:8499551 DvMF_0321 iron-containing alcohol
           dehydrogenase (RefSeq)
          Length = 393

 Score =  408 bits (1049), Expect = e-118
 Identities = 213/384 (55%), Positives = 268/384 (69%), Gaps = 6/384 (1%)

Query: 6   FFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDNMLTKLGMAGDVQKALEERNIFSVIY 65
           FFIPSV +IG  +       +   G ++ LIVTD  + K G+   +   L+   +   +Y
Sbjct: 10  FFIPSVTLIGIGASKAIPEKIKALGGSKPLIVTDMGIVKAGILKQITDLLDAAKMAYSVY 69

Query: 66  DGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHDCAKGIALVAANGGDIRDYEGVDR 125
           D T PNPT +NV  G+++ K+N CDS+I+LGGGS HDC KGI LV ANGG I D+EGVD+
Sbjct: 70  DETIPNPTDDNVHKGVEVYKKNKCDSLITLGGGSSHDCGKGIGLVIANGGKIHDFEGVDK 129

Query: 126 SAKPQLPMIAINTTAGTASEMTRFCIITDEARHIKMAIVDKHVTPLLSVNDSSLMIGMPK 185
           S KP  P +A+NTTAGTASEMTRFCIITD +R +KMAIVD  VTP ++++D  LM+GMP 
Sbjct: 130 SFKPMPPYVAVNTTAGTASEMTRFCIITDTSRKVKMAIVDWRVTPSIALDDPLLMMGMPP 189

Query: 186 SLTAATGMDALTHAIEAYVSIAATPITDACALKAVTMIAENLPLAVEDGSNAKAREAMAY 245
           +LTAATGMDALTHA+EAYVS  ATP+TDACA +A+T+IA  L  AV +G + +ARE M +
Sbjct: 190 ALTAATGMDALTHAVEAYVSTIATPMTDACAEQAITLIATFLRRAVANGRDIEARERMCF 249

Query: 246 AQFLAGMAFNNASLGYVHAMAHQLGGFYNLPHGVCNAVLLPHVQVFNSKVAAARLRDCAA 305
           AQ+LAGMAFNNASLG+VHAMAHQLGGFY+LPHG CNA+LLPHV  FN      R    A 
Sbjct: 250 AQYLAGMAFNNASLGHVHAMAHQLGGFYDLPHGECNAILLPHVSQFNLIAKLDRFARIAE 309

Query: 306 AMGVNVTGKNDAEGAEACINAIRELAKKVDIPAGLRDL------NVKEEDFAVLATNALK 359
            MG N++G +  + AE  I AI+ L+  V IPAGL  L      +VK +D A++  NA K
Sbjct: 310 LMGENISGLSVRDAAEKAICAIKRLSADVGIPAGLVALGKRYGKDVKAKDIAIMTKNAQK 369

Query: 360 DACGFTNPIQATHEEIVAIYRAAM 383
           DACG TNP   T  ++ AIY AAM
Sbjct: 370 DACGLTNPRCPTDADVAAIYEAAM 393


Lambda     K      H
   0.318    0.131    0.373 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 449
Number of extensions: 20
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 383
Length of database: 393
Length adjustment: 30
Effective length of query: 353
Effective length of database: 363
Effective search space:   128139
Effective search space used:   128139
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 50 (23.9 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory