Aligments for a candidate for treEIIA in Desulfovibrio vulgaris Miyazaki F

GapMind for catabolism of small carbon sources

Aligments for a candidate for treEIIA in Desulfovibrio vulgaris Miyazaki F

Align trehalose-specific PTS system, I, HPr, and IIA components (characterized)
to candidate 8502167 DvMF_2877 phosphoenolpyruvate-protein phosphotransferase (RefSeq)

Query= reanno::pseudo3_N2E3:AO353_15995
         (844 letters)



>FitnessBrowser__Miya:8502167
          Length = 591

 Score =  316 bits (809), Expect = 3e-90
 Identities = 195/567 (34%), Positives = 300/567 (52%), Gaps = 17/567 (2%)

Query: 272 VSTKLLRGVCASAGSAFGYVVQVAERTLEM-----PEFAADQQLERESLERALMHATQAL 326
           ++ ++L+G+  SAG + G       R   +     P  AAD ++ R  LE+A       L
Sbjct: 1   MAREILQGIAVSAGVSIGKAFFTNRRRRSVSFEIIPPGAADSEVAR--LEQAASDVRDDL 58

Query: 327 QRLRDNAAGEAQ--ADIFKAHQELLEDPSLLEQAQALIAEGKSAA-FAWNSATEATATLF 383
            R RDN   E +  A I  +H  + +DP L+  A   I +    A +A + A EA A  F
Sbjct: 59  ARARDNVPAELRDHAAIINSHMMICQDPKLIRAASQRIRDHHICAEWALDQAVEAIAAAF 118

Query: 384 KSLGSTLLAERALDLMDVGQRVLKLILGVPDGVWELPDQAI-LIAEQLTPSQTAALDTGK 442
            ++    + ER  D+  V +R+   ++G        PD+ I L+A  LTP+    L T K
Sbjct: 119 SAIEDPYIRERVQDVRAVAERIQTRLVGRHAEPLRTPDERIVLMAHDLTPADAMGLPTVK 178

Query: 443 VLGFATVGGGATSHVAILARALGLPAVCGLPLQVLSLASGTRVLLDADKGELHLDPAVSV 502
           ++ FAT  GG TSH  ILAR+L +PAV G+     S+A G  V++DA +G + +DP  + 
Sbjct: 179 IMSFATAEGGKTSHTGILARSLQIPAVVGVSSLEESVADGDLVIVDALRGLIVVDPDETE 238

Query: 503 IEQLHAKRQQQRQRHQHELENAARAAVTRDGHHFEVTANVASLAETEQAMSLGAEGIGLL 562
           +      + Q     +     +   A T DG+  +V AN+  + E  Q +  G +G+GL 
Sbjct: 239 LADYTDLKYQFENYQKSIRRQSTLPAETLDGYRIDVQANIELVEEVPQVLDGGGDGVGLY 298

Query: 563 RSEFLYQQRSVAPSHDEQAGTYSAIARALGPQRNLVVRTLDVGGDKPLAYVPMDSEANPF 622
           R+E+ +  R  +P+ +E    YS +A  L P+R +V RTLDVG DK L+      E NP 
Sbjct: 299 RTEYAFLNRRTSPTEEELYDEYSQLAGLLSPRR-VVFRTLDVGADKMLSEQSQLEEPNPA 357

Query: 623 LGMRGIRLCLERPQLLREQFRAILSSAGLARLHIMLPMVSQLSELRLARLMLEEEALALG 682
           LG+R IR CL    + R Q RAIL ++      ++ PM+S L ELR A+ +L E    L 
Sbjct: 358 LGLRAIRFCLRHQDVFRRQLRAILRASAHGSASLLFPMISGLHELRQAKHILNEVRAELD 417

Query: 683 LRELP-----KLGIMIEVPAAALMADLFAPEVDFFSIGTNDLTQYTLAMDRDHPRLASQA 737
              LP      +GIM+E+P+A ++++  A EVDFFSIGTNDL QY+L +DR +  ++   
Sbjct: 418 AAGLPYDPDMPVGIMVELPSAVMISEALAQEVDFFSIGTNDLIQYSLGIDRGNKHVSYLY 477

Query: 738 DSFHPSVLRLIASTVKAAHAHGKWVGVCGALASETLAVPLLLGLGVDELSVSVPLIPAIK 797
              HP+++R I   V +AH  G  V VCG +AS+   +P+LLG+ +D +S++   IP IK
Sbjct: 478 QPLHPAIVRSIKYVVDSAHRMGISVCVCGEVASDPYCLPILLGMQIDAISIAPQAIPGIK 537

Query: 798 AAIREVELSDCQAIAHQVLGLESAEQV 824
             +R   + +C+ +   VL   +   +
Sbjct: 538 HILRRTNMEECKELLRDVLSASTVSTI 564


Lambda     K      H
   0.318    0.132    0.370 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 974
Number of extensions: 47
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 844
Length of database: 591
Length adjustment: 39
Effective length of query: 805
Effective length of database: 552
Effective search space:   444360
Effective search space used:   444360
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 54 (25.4 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources