Align Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; Acetate--CoA ligase; Acyl-activating enzyme; EC 6.2.1.1 (characterized)
to candidate Dsui_2003 Dsui_2003 acetate--CoA ligase
Query= SwissProt::P31638 (660 letters) >FitnessBrowser__PS:Dsui_2003 Length = 658 Score = 1028 bits (2659), Expect = 0.0 Identities = 498/660 (75%), Positives = 565/660 (85%), Gaps = 5/660 (0%) Query: 1 MSAIESVMQEHRVFNPPEGFASQAAIPSMEAYQALCDEAERDYEGFWARHARELLHWTKP 60 MS IESV+ E+RVF P E +A I M AY+ALC EAE DY G+W R A+E + W +P Sbjct: 1 MSQIESVLVENRVFPPSEEVVKRATISGMAAYEALCKEAETDYTGYWGRLAKEHVVWKQP 60 Query: 61 FTKVLDQSNAPFYKWFEDGELNASYNCLDRNLQNGNADKVAIVFEADDGSVTRVTYRELH 120 FT VLD+SNAPFYKWF DG+LN SYNCLD+N++ G DKVAI+FEAD G+V++VTY+EL Sbjct: 61 FTSVLDESNAPFYKWFADGKLNVSYNCLDKNVEAGLGDKVAIIFEADGGAVSKVTYKELL 120 Query: 121 GKVCRFANGLKALGIRKGDRVVIYMPMSVEGVVAMQACARLGATHSVVFGGFSAKSLQER 180 +V +FAN LKA GI+KGDRV+IY+PMS+EG+VAMQACAR+GA HSVVFGGFSAKSL+ER Sbjct: 121 SRVAKFANALKAKGIKKGDRVIIYLPMSIEGIVAMQACARIGAIHSVVFGGFSAKSLEER 180 Query: 181 LVDVGAVALITADEQMRGGKALPLKAIADDALALGGCEA-VRNVIVYRRTGGKVAWTEGR 239 ++D GAVA+ITADEQ RGGK +PLK D+AL L G + V+ VIV +RTGG GR Sbjct: 181 ILDAGAVAVITADEQTRGGKNIPLKPAVDEALTLVGANSPVKTVIVAKRTGGACNMVAGR 240 Query: 240 DRWMEDVSAGQPDTCEAEPVSAEHPLFVLYTSGSTGKPKGVQHSTGGYLLWALMTMKWTF 299 D W D AGQ D CE E V AEHPLF+LYTSGSTGKPKGVQHS+GGYLL A +TMKWTF Sbjct: 241 DVWWTDAEAGQSDICEPEWVEAEHPLFLLYTSGSTGKPKGVQHSSGGYLLHAALTMKWTF 300 Query: 300 DIKPDDLFWCTADIGWVTGHTYIAYGPLAAGATQVVFEGVPTYPNAGRFWDMIARHKVSI 359 DIKPDD+FWCTADIGWVTGHTYI YGPLA GAT++VFEGVPT+P+AGRFW MI HKVSI Sbjct: 301 DIKPDDVFWCTADIGWVTGHTYITYGPLACGATEIVFEGVPTFPDAGRFWKMIQDHKVSI 360 Query: 360 FYTAPTAIRSLIKAAEADEKIHPKQYDLSSLRLLGTVGEPINPEAWMWYYKNIGNERCPI 419 FYTAPTAIRSLIKA AD PK+YDL+SLRLLG+VGEPINPEAWMWYY IG RCPI Sbjct: 361 FYTAPTAIRSLIKAG-ADL---PKKYDLTSLRLLGSVGEPINPEAWMWYYNEIGGGRCPI 416 Query: 420 VDTFWQTETGGHMITPLPGATPLVPGSCTLPLPGIMAAIVDETGHDVPNGNGGILVVKRP 479 VDTFWQTETGGHMITPLPG TPLVPGSCTLP PGI AAIVDETGH++ G GG LVVK+P Sbjct: 417 VDTFWQTETGGHMITPLPGVTPLVPGSCTLPFPGIQAAIVDETGHELEWGKGGFLVVKKP 476 Query: 480 WPAMIRTIWGDPERFRKSYFPEELGGKLYLAGDGSIRDKDTGYFTIMGRIDDVLNVSGHR 539 WP+MIRTIWGDPERF+KSY+PE+LGG+LYLAGDG++R+ TG FTI GRIDDVLNVSGHR Sbjct: 477 WPSMIRTIWGDPERFKKSYYPEDLGGRLYLAGDGAVRNAKTGNFTITGRIDDVLNVSGHR 536 Query: 540 MGTMEIESALVSNPLVAEAAVVGRPDDMTGEAICAFVVLKRSRPTGEEAVKIATELRNWV 599 MGTMEIESALV+NPLVAEAAVVGRPDD+TGEAICAFVVLK SRP+GE+A ++ EL++WV Sbjct: 537 MGTMEIESALVANPLVAEAAVVGRPDDLTGEAICAFVVLKGSRPSGEDAKRVIKELQDWV 596 Query: 600 GKEIGPIAKPKDIRFGDNLPKTRSGKIMRRLLRSLAKGEEITQDTSTLENPAILEQLKQA 659 GKEIGPIAKPKDIRFG+NLPKTRSGKIMRRLLRSLAKGEE+TQD STLENPAILEQLKQA Sbjct: 597 GKEIGPIAKPKDIRFGENLPKTRSGKIMRRLLRSLAKGEEVTQDVSTLENPAILEQLKQA 656 Lambda K H 0.319 0.136 0.422 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1552 Number of extensions: 80 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 660 Length of database: 658 Length adjustment: 38 Effective length of query: 622 Effective length of database: 620 Effective search space: 385640 Effective search space used: 385640 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 54 (25.4 bits)
Align candidate Dsui_2003 Dsui_2003 (acetate--CoA ligase)
to HMM TIGR02188 (acs: acetate--CoA ligase (EC 6.2.1.1))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.carbon/TIGR02188.hmm # target sequence database: /tmp/gapView.16587.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR02188 [M=629] Accession: TIGR02188 Description: Ac_CoA_lig_AcsA: acetate--CoA ligase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 8.8e-297 971.4 0.0 1e-296 971.2 0.0 1.0 1 lcl|FitnessBrowser__PS:Dsui_2003 Dsui_2003 acetate--CoA ligase Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__PS:Dsui_2003 Dsui_2003 acetate--CoA ligase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 971.2 0.0 1e-296 1e-296 3 628 .. 28 655 .. 26 656 .. 0.97 Alignments for each domain: == domain 1 score: 971.2 bits; conditional E-value: 1e-296 TIGR02188 3 eleeykelyeeaiedpekfwaklakeelewlkpfekvldeslepkvkWfedgelnvsyncvdrhvek.rkdkvaiiw 78 + +y++l++ea++d++ +w++lake++ w++pf++vldes++p++kWf+dg+lnvsync+d++ve+ dkvaii+ lcl|FitnessBrowser__PS:Dsui_2003 28 GMAAYEALCKEAETDYTGYWGRLAKEHVVWKQPFTSVLDESNAPFYKWFADGKLNVSYNCLDKNVEAgLGDKVAIIF 104 6789***************************************************************99******** PP TIGR02188 79 egdeegedsrkltYaellrevcrlanvlkelGvkkgdrvaiYlpmipeaviamlacaRiGavhsvvfaGfsaealae 155 e+d g + k+tY+ell++v+++an+lk+ G+kkgdrv+iYlpm +e ++am+acaRiGa+hsvvf+Gfsa++l+e lcl|FitnessBrowser__PS:Dsui_2003 105 EAD-GG-AVSKVTYKELLSRVAKFANALKAKGIKKGDRVIIYLPMSIEGIVAMQACARIGAIHSVVFGGFSAKSLEE 179 ***.44.48******************************************************************** PP TIGR02188 156 RivdaeaklvitadeglRggkvielkkivdealekaee..svekvlvvkrtgeevaewkegrDvwweelvekeasae 230 Ri da a vitade++Rggk+i+lk +vdeal+ + + v++v+v krtg +++ grDvww ++ + ++s+ lcl|FitnessBrowser__PS:Dsui_2003 180 RILDAGAVAVITADEQTRGGKNIPLKPAVDEALTLVGAnsPVKTVIVAKRTGGA-CNMVAGRDVWWTDAEA-GQSDI 254 *********************************98776568*************.55************99.6**** PP TIGR02188 231 cepekldsedplfiLYtsGstGkPkGvlhttgGylllaaltvkyvfdikdedifwCtaDvGWvtGhsYivygPLanG 307 cepe++++e+plf+LYtsGstGkPkGv+h++gGyll+aalt+k++fdik++d+fwCtaD+GWvtGh+Yi ygPLa+G lcl|FitnessBrowser__PS:Dsui_2003 255 CEPEWVEAEHPLFLLYTSGSTGKPKGVQHSSGGYLLHAALTMKWTFDIKPDDVFWCTADIGWVTGHTYITYGPLACG 331 ***************************************************************************** PP TIGR02188 308 attllfegvptypdasrfweviekykvtifYtaPtaiRalmklgeelvkkhdlsslrvlgsvGepinpeaweWyyev 384 at+++fegvpt+pda+rfw++i+ +kv+ifYtaPtaiR+l+k+g +l+kk+dl slr+lgsvGepinpeaw+Wyy++ lcl|FitnessBrowser__PS:Dsui_2003 332 ATEIVFEGVPTFPDAGRFWKMIQDHKVSIFYTAPTAIRSLIKAGADLPKKYDLTSLRLLGSVGEPINPEAWMWYYNE 408 ***************************************************************************** PP TIGR02188 385 vGkekcpivdtwWqtetGgilitplpgvatelkpgsatlPlfGieaevvdeegkeveeeeeggvLvikkpwPsmlrt 461 +G ++cpivdt+WqtetGg++itplpg +t+l pgs+tlP++Gi+a++vde+g+e+e +++ g+Lv+kkpwPsm+rt lcl|FitnessBrowser__PS:Dsui_2003 409 IGGGRCPIVDTFWQTETGGHMITPLPG-VTPLVPGSCTLPFPGIQAAIVDETGHELEWGKG-GFLVVKKPWPSMIRT 483 ***************************.5*****************************999.8************** PP TIGR02188 462 iygdeerfvetYfkklkg..lyftGDgarrd.kdGyiwilGRvDdvinvsGhrlgtaeiesalvsheavaeaavvgv 535 i+gd+erf ++Y+ + g ly++GDga+r+ k G ++i+GR+Ddv+nvsGhr+gt+eiesalv+++ vaeaavvg+ lcl|FitnessBrowser__PS:Dsui_2003 484 IWGDPERFKKSYYPEDLGgrLYLAGDGAVRNaKTGNFTITGRIDDVLNVSGHRMGTMEIESALVANPLVAEAAVVGR 560 *************9876668********987256******************************************* PP TIGR02188 536 pdeikgeaivafvvlkegveedee..elekelkklvrkeigpiakpdkilvveelPktRsGkimRRllrkiaegeel 610 pd+++geai+afvvlk ++ + e+ ++ kel+++v keigpiakp++i++ e+lPktRsGkimRRllr++a+gee+ lcl|FitnessBrowser__PS:Dsui_2003 561 PDDLTGEAICAFVVLKGSRPSGEDakRVIKELQDWVGKEIGPIAKPKDIRFGENLPKTRSGKIMRRLLRSLAKGEEV 637 ****************99875554449************************************************** PP TIGR02188 611 lgdvstledpsvveelke 628 ++dvstle+p+++e+lk+ lcl|FitnessBrowser__PS:Dsui_2003 638 TQDVSTLENPAILEQLKQ 655 ***************997 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (629 nodes) Target sequences: 1 (658 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.02u 0.02s 00:00:00.04 Elapsed: 00:00:00.03 # Mc/sec: 12.94 // [ok]
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory