Align ABC transporter for L-Lysine, permease component 1 (characterized)
to candidate Dsui_0636 Dsui_0636 amine acid ABC transporter, permease protein, 3-TM region, His/Glu/Gln/Arg/opine family
Query= reanno::pseudo5_N2C3_1:AO356_09905 (231 letters) >FitnessBrowser__PS:Dsui_0636 Length = 245 Score = 110 bits (274), Expect = 3e-29 Identities = 67/211 (31%), Positives = 110/211 (52%), Gaps = 1/211 (0%) Query: 12 LAAGALMTVKLALSALCLGLVLGLLGALAKTSPYKPLQWLGGTYSTLVRGIPELLWVLLI 71 L G TV L+LSA + LV+G + + +T P + L Y R +P L+ + Sbjct: 25 LFTGLQWTVALSLSAWVIALVVGSIVGVLRTVPNRWLSGFAAVYVECFRNVPLLVQLFSW 84 Query: 72 YFGTVNLMR-ALGEYLGMPDLALNAFAAGVIALGLCFGAYATEVFRGAILAIPKGHREAG 130 YF L+ ALG D L F A ++ LGL A E R I ++P+G R AG Sbjct: 85 YFVLPELLPPALGNAYKQSDPLLQQFLAAMLCLGLFTAARVAEQVRAGIESLPRGQRNAG 144 Query: 131 VALGLSKWRIFTRLIMPQMWRIALPGLGNLFMILMKDTALVSVIGLEEIMRHAQIGVTVS 190 +A+G + +++ +++P +RI +P L + F+ + K++A+ + IGL E+ R AQ V + Sbjct: 145 LAMGFTLAQVYRHVLLPMAFRIIVPPLTSEFLNIFKNSAVATTIGLIELSRQAQQLVDYT 204 Query: 191 KQPFTFYMVAALMYLGLTVLAMLGMHLLERR 221 QP+ ++ L+Y+ + V M M LE + Sbjct: 205 AQPYEAFIAVTLLYVCINVTVMFLMRRLEEK 235 Lambda K H 0.329 0.143 0.435 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 156 Number of extensions: 7 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 231 Length of database: 245 Length adjustment: 23 Effective length of query: 208 Effective length of database: 222 Effective search space: 46176 Effective search space used: 46176 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 46 (22.3 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory