Align mannitol 2-dehydrogenase (NADP+) (EC 1.1.1.138) (characterized)
to candidate Dsui_2228 Dsui_2228 Zn-dependent alcohol dehydrogenase
Query= BRENDA::Q1ACW3 (345 letters) >FitnessBrowser__PS:Dsui_2228 Length = 340 Score = 103 bits (258), Expect = 5e-27 Identities = 77/218 (35%), Positives = 106/218 (48%), Gaps = 11/218 (5%) Query: 20 VVEVPLPTLRDNDVLIKVKACGVCGTDLHIHEGEFIAK--FPLIPGHETVGVVAAIGKDV 77 + EVP+P + VL+KV A GVC TDLH +G++ K P IPGHE VG VAA+G V Sbjct: 19 IEEVPVPEVPPGQVLVKVVASGVCHTDLHAADGDWPVKPSLPFIPGHEGVGYVAAVGAGV 78 Query: 78 KGFTVGERVCAD-NSELCNECFYCRRGQLLLCEKFEAHGVTMDGGFAEYCAYPAGKVFKI 136 G+RV C C +C G LC+ + G T++GG+AEY G V K+ Sbjct: 79 THVKEGDRVGVPWLHTACGHCEHCITGWETLCDSQQMTGYTVNGGYAEYVLADPGYVGKL 138 Query: 137 HNLSDVDATLLEPASCAA----HGLEKIAPKIGSSVLMFGAGPTGLCLAQLPHNGASHVV 192 + ++ P CA GL+ + K G V + G G G Q HV+ Sbjct: 139 PD--TLEFAPAAPVLCAGVTVYKGLKVLECKPGDWVAISGIGGLGHMAVQYAKAMGFHVI 196 Query: 193 IAAPEGLKMDLAKKLDCADIYVPLSRSNPQAQFD-QIK 229 K+ LAK L AD+ + +R +P A+ QIK Sbjct: 197 AVDVADEKLALAKTLG-ADVTLNAARVDPVAEIQKQIK 233 Lambda K H 0.320 0.138 0.420 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 302 Number of extensions: 22 Number of successful extensions: 4 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 345 Length of database: 340 Length adjustment: 29 Effective length of query: 316 Effective length of database: 311 Effective search space: 98276 Effective search space used: 98276 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 49 (23.5 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory