Alignments for a candidate for ppa in Dechlorosoma suillum PS

GapMind for catabolism of small carbon sources

Alignments for a candidate for ppa in Dechlorosoma suillum PS

Align Phenylacetate permease, Ppa (characterized)
to candidate Dsui_0290 Dsui_0290 SSS sodium solute transporter

Query= TCDB::O50471
         (520 letters)



>FitnessBrowser__PS:Dsui_0290
          Length = 582

 Score =  675 bits (1741), Expect = 0.0
 Identities = 340/548 (62%), Positives = 414/548 (75%), Gaps = 29/548 (5%)

Query: 2   NWTAISMFMVFVCFTLLVTRWAALRTRSASDFYTAGGGLTGMQNGLAIAGDMISAASFLG 61
           NWTAI MF  FV  TL +T+WAA +T++A+DFYTAGGG+TG QNGLAIAGD +SAASFLG
Sbjct: 35  NWTAIIMFGAFVLATLWITKWAASKTKTAADFYTAGGGITGFQNGLAIAGDYMSAASFLG 94

Query: 62  ISAMMFMNGYDGLLYALGVLAGWPIILFLIAERLRNLGKYTFADVVSYRLAQTPVRLTSA 121
           ISA +F NGYDGL+Y++G L GWPII+FL+AERLRNLGK+TFADV +YR   TP+R  +A
Sbjct: 95  ISAAVFANGYDGLIYSIGFLVGWPIIMFLMAERLRNLGKFTFADVAAYRFQATPIRALAA 154

Query: 122 FGTLVVALMYLVAQMVGAGKLIELLFGISYLYAVMLVGVLMVAYVTFGGMLATTWVQIIK 181
            GTLVV   YL+AQMVGAG+LI+LLFG+ Y  AV+LVG+LM+ YV FGGM ATTWVQIIK
Sbjct: 155 SGTLVVVAFYLIAQMVGAGQLIKLLFGLDYWMAVVLVGILMMVYVLFGGMTATTWVQIIK 214

Query: 182 AVMLLSGTSFMAFMVLKHFGFSTEAMFASAV----------------------------- 212
           AV+LLSG SFM FMVL  +GFS EA+FA AV                             
Sbjct: 215 AVLLLSGASFMVFMVLAKYGFSPEALFADAVRIKTDLAAKGLLAKALETDPSATAETVAA 274

Query: 213 AVHAKGQAIMAPGGLLSNPVDAISLGLGMMFGTAGLPHILMRFFTVSDAKEARKSVFYAT 272
           A  AKGQ+IM PG  + +P+ AIS G+ +MFGTAGLPHILMRFFTV D KEARKSVF+AT
Sbjct: 275 AAAAKGQSIMGPGSFIKDPISAISFGMALMFGTAGLPHILMRFFTVPDGKEARKSVFWAT 334

Query: 273 GFIGYFYLLLIVIGFGAIVMVGTEPSYRDATGAIIGGGNMIAVHLAQAVGGNLFLGFISA 332
            +IGYFY+L  +IGFGAIV+VGT P + DA G + GGGNM A+HLA AVGGN+FLGFISA
Sbjct: 335 TWIGYFYILTFIIGFGAIVLVGTNPEFLDAKGVLKGGGNMAAIHLANAVGGNVFLGFISA 394

Query: 333 VAFATILAVVAGLALSGASAVSHDLYACVIRQGKATEQEEMRVSRIATLLIGLLAVLLGL 392
           VAFATILAVVAGL LSGASAVSHDLYA V R G      EMRVS++ T+ +G++AV+LG+
Sbjct: 395 VAFATILAVVAGLTLSGASAVSHDLYASVFRHGNVDSATEMRVSKMTTVALGIIAVVLGI 454

Query: 393 MFESQNIAFLSGLVLAVAASVNFPVLLLSMFWKGLTTRGAVCGSMAGLASAVLLVVLGPA 452
            FE QNIAF+  L  A+AAS NFPVL +S+ WK  TT+GA  G   GL +AV+L VL  +
Sbjct: 455 AFEKQNIAFMVSLAFAIAASANFPVLFMSVLWKDCTTKGAFYGGFLGLITAVVLTVLSKS 514

Query: 453 VWVNVLHHEKALFPYSNPALFSMSLAFLSAWVFSVTDSSERASEERGRYLAQFIRSMTGI 512
           +WV++L ++  +FPY++PALFSM+  F+  W+FS+ D SE+A++ER  YL Q IRS TGI
Sbjct: 515 IWVDILGNKTEIFPYASPALFSMAAGFIGIWLFSLMDRSEQAAKERVAYLDQEIRSETGI 574

Query: 513 GAAGASKH 520
           GAA AS H
Sbjct: 575 GAAAASSH 582


Lambda     K      H
   0.328    0.139    0.407 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 945
Number of extensions: 40
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 520
Length of database: 582
Length adjustment: 36
Effective length of query: 484
Effective length of database: 546
Effective search space:   264264
Effective search space used:   264264
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources