Aligments for a candidate for aacS in Dechlorosoma suillum PS

GapMind for catabolism of small carbon sources

Aligments for a candidate for aacS in Dechlorosoma suillum PS

Align Acetoacetate--CoA ligase (EC 6.2.1.16) (characterized)
to candidate Dsui_3212 Dsui_3212 acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II

Query= reanno::acidovorax_3H11:Ac3H11_3009
         (578 letters)



>FitnessBrowser__PS:Dsui_3212
          Length = 555

 Score =  213 bits (543), Expect = 1e-59
 Identities = 165/542 (30%), Positives = 256/542 (47%), Gaps = 17/542 (3%)

Query: 29  QTIGAFFADMVARQPEREALVSVHQGRRYTYAQLQTEAHRLASALLG-MGLTPGDRVGIW 87
           +++G  F    A+  +R A +++  G   TY +L   +   A+ L   + L  G RV + 
Sbjct: 23  KSLGQLFEQSCAQYRDRVAYINMGVG--ITYGELDRLSRDFAAYLQDVLKLPQGARVALM 80

Query: 88  SHNNAEWVLMQLATAQVGLVLVNINPAYRTAEVEYALNKVGCKLLVSMARFKTS--DYLG 145
             N  ++ +      + G V+VN NP Y   E+E+ L   G + +V +  F  +    L 
Sbjct: 81  MPNLLQYPVCMFGALRAGYVVVNCNPLYTHRELEHQLKDSGAEAIVIVENFAHTLEQALP 140

Query: 146 MLRELAPEWQGQQPGHLQAAKLPQLKTVVWIDDEAGQGADEPGLLRFTELIARGNAADPR 205
           ++  L           L A K   +  VV    +       P  ++F   +ARG  A  R
Sbjct: 141 LVPGLKHVIVTSLGDMLGALKGTVVNLVVRHVKKMVPAWKLPRHVKFKAAMARGKGATLR 200

Query: 206 LAQVAAGLQATDPINIQFTSGTTGFPKGATLTHRNILNNGFFIGECMK--LTPADRLCIP 263
             QV       D   +Q+T GTTG  KGA L HRNI+ N       ++  L    +L I 
Sbjct: 201 PVQVGH----EDIAYLQYTGGTTGVAKGAMLLHRNIIANLQQAHAWIEPFLHKDQQLIIT 256

Query: 264 -VPLYHCFGMVLGNLACFTHGATIVYPNDGFDPLTVLQTVQDERCTGLHGVPTMFIAELD 322
            +PLYH F +    L     GAT V   +  D    ++ +   + T + GV T+F A L+
Sbjct: 257 ALPLYHIFSLTANCLTFLKIGATNVLITNPRDIPGFVKELAQYKFTVITGVNTLFNALLN 316

Query: 323 HPRFAEFNLSTLRTGIMAGSPCPTEVMKRVVEQMNLREITIAYGMTETSPVSCQSSTDTP 382
           +P FA+ + S LR  +  G      V ++   Q+  + +  AYG+TETSP +  +  D  
Sbjct: 317 NPDFAKLDFSALRAALGGGMAVQKSVAQKW-RQVTGKPLIEAYGLTETSPAATINPLD-- 373

Query: 383 LSKRVSTVGQVQPHLEVKIVDPDTGAVVPIGQRGEFCTKGYSVMHGYWGDEAKTREAIDE 442
           L +    +G      E+ I D D G  +P+GQ GE C +G  VM GYW    +T      
Sbjct: 374 LGEFNGAIGLPISSTEIVIRD-DLGNDLPVGQAGEICIRGPQVMKGYWLRPDETATVFYA 432

Query: 443 GGWMHTGDLATMDAEGYVNIVGRIKDMVIRGGENIYPREIEEFLYRHPQVQDVQVVGVPD 502
            G++ TGD+  MD +G+V IV R KDM++  G N+YP E+E  +  HP V +V  VGVP 
Sbjct: 433 DGFLRTGDVGVMDEKGFVRIVDRKKDMILVSGFNVYPNEVEAVVAMHPAVMEVAAVGVPS 492

Query: 503 QKYGEELCAWIIAKPGTQPTEDDIRAFCKGQIAHYKVPRYIRFVTSFPMTVTGKIQKFKI 562
           +  GE +  +++ K     T++ + A CK  +  YKVP  + F    P T  GKI +  +
Sbjct: 493 EHSGEAVKIFVVLK-DKSVTKEQLIAHCKENLTGYKVPHLVEFRDDLPKTNVGKILRRAL 551

Query: 563 RD 564
           ++
Sbjct: 552 KE 553


Lambda     K      H
   0.320    0.136    0.412 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 715
Number of extensions: 39
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 578
Length of database: 555
Length adjustment: 36
Effective length of query: 542
Effective length of database: 519
Effective search space:   281298
Effective search space used:   281298
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources