GapMind for catabolism of small carbon sources

 

Aligments for a candidate for bamI in Dechlorosoma suillum PS

Align Iron-sulfur cluster-binding protein, putative (characterized, see rationale)
to candidate Dsui_3029 Dsui_3029 NADH:ubiquinone oxidoreductase chain G-like protein

Query= uniprot:Q39TW6
         (218 letters)



>lcl|FitnessBrowser__PS:Dsui_3029 Dsui_3029 NADH:ubiquinone
           oxidoreductase chain G-like protein
          Length = 237

 Score = 79.7 bits (195), Expect = 4e-20
 Identities = 53/173 (30%), Positives = 79/173 (45%), Gaps = 29/173 (16%)

Query: 8   IDGKEVVATEGMTILDAAKSVGISIPTLCHHEKLEPYGGCRICTVEVEVRGWPKLVAGCI 67
           +DGK VV  +G TIL+AA+  G  IP LC H     +G C++C V+V  R     V+ C 
Sbjct: 9   LDGKPVVFQDGQTILEAARQAGHYIPHLCWHPDFPAHGSCKLCIVKVGGRH----VSSCA 64

Query: 68  YPVEKGLVVRTRNEKIDKIRKVLLE-------------------EMLAHAPDSEELKALA 108
            P ++G+ V +   +++  R+ LL+                   ++ A A D E L A  
Sbjct: 65  MPAKEGMEVESNTPEMNGERRALLQMLFVEGNHFCPSCEKSGNCQLQALAYDLEMLSAHF 124

Query: 109 QEYGADRDRFEKHPSF------CIHCGLCVRYCAEIKKKNAVGFVDRGSNREI 155
             +  +R     HP        CI C LCVR   +I  KN     DRG ++ +
Sbjct: 125 NHFYPNRPVDASHPDVLLDFNRCIFCELCVRASRDIDGKNIFALTDRGIHKHL 177


Lambda     K      H
   0.320    0.137    0.432 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 150
Number of extensions: 7
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 218
Length of database: 237
Length adjustment: 22
Effective length of query: 196
Effective length of database: 215
Effective search space:    42140
Effective search space used:    42140
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 46 (22.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory