GapMind for catabolism of small carbon sources

 

Aligments for a candidate for bamI in Dechlorosoma suillum PS

Align Iron-sulfur cluster-binding protein, putative (characterized, see rationale)
to candidate Dsui_3044 Dsui_3044 NADH-quinone oxidoreductase, chain G

Query= uniprot:Q39TW6
         (218 letters)



>lcl|FitnessBrowser__PS:Dsui_3044 Dsui_3044 NADH-quinone
           oxidoreductase, chain G
          Length = 776

 Score = 92.8 bits (229), Expect = 2e-23
 Identities = 63/205 (30%), Positives = 97/205 (47%), Gaps = 31/205 (15%)

Query: 4   INLQIDGKEVVATEGMTILDAAKSVGISIPTLCHHEKLEPYGGCRICTVEVEVRGWPKLV 63
           + ++IDGK+    +G T++DAA+ VG+ IP  C+H+KL     CR+C V+VE    PK +
Sbjct: 2   LEIEIDGKKAEVPDGSTVMDAAQQVGVYIPHFCYHKKLSIAANCRMCLVQVEKA--PKPL 59

Query: 64  AGCIYPVEKGLVVRTRNEKIDKIRKVLLEEMLAHAP---------DSEELKALAQEYGAD 114
             C  PV  G+ V T +E   K +K ++E +L + P            +L+ L+  YG  
Sbjct: 60  PACATPVTNGMKVFTHSELAVKAQKGVMEFLLINHPLDCPICDQGGECQLQDLSVGYGGV 119

Query: 115 RDRFEKHP-----------------SFCIHCGLCVRYCAEIKKKNAVGFVDRGSNREI-S 156
           + R+++                   S CIHC  CVR+  EI     +G  +R  + EI +
Sbjct: 120 KSRYQEEKRVVFHKDIGPLISMEEMSRCIHCTRCVRFGQEIAGIMELGMANRNFHSEITT 179

Query: 157 FIPEIAAKECWDCKECFPLCPTSAL 181
           F+      E         LCP  AL
Sbjct: 180 FMARTVDSEL--SGNAIDLCPVGAL 202


Lambda     K      H
   0.320    0.137    0.432 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 335
Number of extensions: 17
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 218
Length of database: 776
Length adjustment: 31
Effective length of query: 187
Effective length of database: 745
Effective search space:   139315
Effective search space used:   139315
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 50 (23.9 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory