Align Cation acetate symporter (characterized, see rationale)
to candidate Dsui_0290 Dsui_0290 SSS sodium solute transporter
Query= uniprot:A0A165ZRB0 (552 letters) >FitnessBrowser__PS:Dsui_0290 Length = 582 Score = 728 bits (1879), Expect = 0.0 Identities = 380/576 (65%), Positives = 446/576 (77%), Gaps = 30/576 (5%) Query: 6 LALLSIAAFAPGAWAAEALTGAVQKQPLNVAAILMFVAFVGATLYITYWASKKNNSAADY 65 LAL + + +AA AL G +KQ N AI+MF AFV ATL+IT WA+ K +AAD+ Sbjct: 8 LALAGLGLLSSPVFAA-ALEGQAEKQATNWTAIIMFGAFVLATLWITKWAASKTKTAADF 66 Query: 66 YAAGGKITGFQNGLAIAGDYMSAASFLGISALVFTSGYDGLIYSIGFLVGWPIILFLIAE 125 Y AGG ITGFQNGLAIAGDYMSAASFLGISA VF +GYDGLIYSIGFLVGWPII+FL+AE Sbjct: 67 YTAGGGITGFQNGLAIAGDYMSAASFLGISAAVFANGYDGLIYSIGFLVGWPIIMFLMAE 126 Query: 126 RLRNLGKYTFADVASYRLGQTQIRSLSASGSLVVVAFYLIAQMVGAGKLIQLLFGLDYHV 185 RLRNLGK+TFADVA+YR T IR+L+ASG+LVVVAFYLIAQMVGAG+LI+LLFGLDY + Sbjct: 127 RLRNLGKFTFADVAAYRFQATPIRALAASGTLVVVAFYLIAQMVGAGQLIKLLFGLDYWM 186 Query: 186 AVILVGILMCLYVLFGGMLATTWVQIIKAVLLLSGASFMALMVMKHVNFDFNMLFAEAVK 245 AV+LVGILM +YVLFGGM ATTWVQIIKAVLLLSGASFM MV+ F LFA+AV+ Sbjct: 187 AVVLVGILMMVYVLFGGMTATTWVQIIKAVLLLSGASFMVFMVLAKYGFSPEALFADAVR 246 Query: 246 VHP-----------------------------KGEAIMSPGGLVKDPISAFSLGLALMFG 276 + KG++IM PG +KDPISA S G+ALMFG Sbjct: 247 IKTDLAAKGLLAKALETDPSATAETVAAAAAAKGQSIMGPGSFIKDPISAISFGMALMFG 306 Query: 277 TAGLPHILMRFFTVSDAKEARKSVLYATGFIGYFYILTFIIGFGAILLVSTNPAFKDAAG 336 TAGLPHILMRFFTV D KEARKSV +AT +IGYFYILTFIIGFGAI+LV TNP F DA G Sbjct: 307 TAGLPHILMRFFTVPDGKEARKSVFWATTWIGYFYILTFIIGFGAIVLVGTNPEFLDAKG 366 Query: 337 ALLGGNNMAAVHLANAVGGSIFLGFISAVAFATILAVVAGLTLAGASAVSHDLYASVIRK 396 L GG NMAA+HLANAVGG++FLGFISAVAFATILAVVAGLTL+GASAVSHDLYASV R Sbjct: 367 VLKGGGNMAAIHLANAVGGNVFLGFISAVAFATILAVVAGLTLSGASAVSHDLYASVFRH 426 Query: 397 GRVNEKDEIRVSKITTIALAVLAITLGILFEKQNIAFMVGLAFSIAASCNFPVLLLSMYW 456 G V+ E+RVSK+TT+AL ++A+ LGI FEKQNIAFMV LAF+IAAS NFPVL +S+ W Sbjct: 427 GNVDSATEMRVSKMTTVALGIIAVVLGIAFEKQNIAFMVSLAFAIAASANFPVLFMSVLW 486 Query: 457 KKLTTRGAMIGGWLGLVSAVGLMILGPTIWVQIMGHEKAIFPYEYPALFSMAIAFVGIWF 516 K TT+GA GG+LGL++AV L +L +IWV I+G++ IFPY PALFSMA F+GIW Sbjct: 487 KDCTTKGAFYGGFLGLITAVVLTVLSKSIWVDILGNKTEIFPYASPALFSMAAGFIGIWL 546 Query: 517 FSITDKSAEGANERALFFPQFVRSQTGLGASGAVNH 552 FS+ D+S + A ER + Q +RS+TG+GA+ A +H Sbjct: 547 FSLMDRSEQAAKERVAYLDQEIRSETGIGAAAASSH 582 Lambda K H 0.328 0.140 0.414 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1015 Number of extensions: 45 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 552 Length of database: 582 Length adjustment: 36 Effective length of query: 516 Effective length of database: 546 Effective search space: 281736 Effective search space used: 281736 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 53 (25.0 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory