Aligments for a candidate for fba in Dechlorosoma suillum PS

GapMind for catabolism of small carbon sources

Aligments for a candidate for fba in Dechlorosoma suillum PS

Align Fructose-bisphosphate aldolase; FBP aldolase; FBPA; Fructose-1,6-bisphosphate aldolase; EC 4.1.2.13 (characterized)
to candidate Dsui_0050 Dsui_0050 fructose-bisphosphate aldolase, class II, Calvin cycle subtype

Query= SwissProt::Q56815
         (354 letters)



>lcl|FitnessBrowser__PS:Dsui_0050 Dsui_0050 fructose-bisphosphate
           aldolase, class II, Calvin cycle subtype
          Length = 354

 Score =  516 bits (1328), Expect = e-151
 Identities = 257/353 (72%), Positives = 296/353 (83%)

Query: 1   MALVSMRQLLDHAADDSYGLPAFNVNNMEQVKAIMDAARATSSPVILQGSAGARKYAGEP 60
           M +VSMRQLLDHAA++ YGLPAFNVNNMEQV AIM+AA   ++PVI+Q SAGARKYAGE 
Sbjct: 1   MPIVSMRQLLDHAAENGYGLPAFNVNNMEQVWAIMEAANELNAPVIMQASAGARKYAGEA 60

Query: 61  FLRHLIAAAVEAYPEIPVVMHQDHGASPAVCMGAIKSGFSSVMMDGSLKEDGKTPADYDY 120
           FLRH I AA+EAYP IPVVMHQDHG SPAVCM AIKSGFSSVMMDGSL+EDGK+ A YDY
Sbjct: 61  FLRHQILAALEAYPHIPVVMHQDHGQSPAVCMAAIKSGFSSVMMDGSLQEDGKSVASYDY 120

Query: 121 NVSVTAKVVELAHAVGVSVEGELGCLGSLETGKGEAEDGHGAEEALDHSKLLTDPDEAAQ 180
           NV+VT +VV+ +HA+GVSVE ELG LGSLET K + EDGHGAE  +    LLTDPD+AA 
Sbjct: 121 NVAVTQEVVKFSHAIGVSVEAELGVLGSLETMKADKEDGHGAEGHMTREDLLTDPDQAAD 180

Query: 181 FVKATQCDALAIAIGTSHGAYKFTRKPTGDILAIDRIKAIHQRIPTTHLVMHGSSSVPQE 240
           FV  T CDALAIAIGTSHGAYKFT+KPTGDILAIDRIK IH RIP THLVMHGSSSVPQ+
Sbjct: 181 FVARTNCDALAIAIGTSHGAYKFTKKPTGDILAIDRIKEIHARIPNTHLVMHGSSSVPQD 240

Query: 241 LLEEIRTYGGDIKETYGVPVEEIQEGIRYGVRKVNIDTDIRLAMTAAIRRVGAKNKSEFD 300
           LL EIR +GGD+KETYGVPVEEI  GI++GVRKVNIDTDIRLAMT A+RR   +N S+FD
Sbjct: 241 LLAEIREFGGDMKETYGVPVEEIVNGIKHGVRKVNIDTDIRLAMTGAVRRYLFENPSKFD 300

Query: 301 PRKFMAAAMEEAKKVCIARFEAFGSAGKAEKIRAIELDEMAKRYASGELAQVV 353
           PR F+  A E AKK+C+AR++AFG  G+A +I+ I L++MA+RY  GEL Q+V
Sbjct: 301 PRDFLKPAREAAKKICVARYQAFGCEGRAGQIKPISLEKMAERYKKGELNQIV 353


Lambda     K      H
   0.316    0.132    0.369 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 446
Number of extensions: 12
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 354
Length of database: 354
Length adjustment: 29
Effective length of query: 325
Effective length of database: 325
Effective search space:   105625
Effective search space used:   105625
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 49 (23.5 bits)

Align candidate Dsui_0050 Dsui_0050 (fructose-bisphosphate aldolase, class II, Calvin cycle subtype)
to HMM TIGR01521 (fba: fructose-bisphosphate aldolase, class II, Calvin cycle subtype (EC 4.1.2.13))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.carbon/TIGR01521.hmm
# target sequence database:        /tmp/gapView.14969.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR01521  [M=347]
Accession:   TIGR01521
Description: FruBisAldo_II_B: fructose-bisphosphate aldolase, class II, Calvin cycle subtype
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                         Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                         -----------
   1.5e-186  605.5   1.0   1.7e-186  605.3   1.0    1.0  1  lcl|FitnessBrowser__PS:Dsui_0050  Dsui_0050 fructose-bisphosphate 


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__PS:Dsui_0050  Dsui_0050 fructose-bisphosphate aldolase, class II, Calvin cycle subtype
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  605.3   1.0  1.7e-186  1.7e-186       2     347 .]       4     349 ..       3     349 .. 1.00

  Alignments for each domain:
  == domain 1  score: 605.3 bits;  conditional E-value: 1.7e-186
                         TIGR01521   2 islrqlldhaaergygvpafnvnnleqilaimeaadktdspvilqasrgarsyagevllrklvlaaveeypdipvvl 78 
                                       +s+rqlldhaae+gyg+pafnvnn+eq+ aimeaa++ ++pvi+qas+gar+yage++lr+ +laa e yp+ipvv+
  lcl|FitnessBrowser__PS:Dsui_0050   4 VSMRQLLDHAAENGYGLPAFNVNNMEQVWAIMEAANELNAPVIMQASAGARKYAGEAFLRHQILAALEAYPHIPVVM 80 
                                       89*************************************************************************** PP

                         TIGR01521  79 hqdhgnspatclsaiqlgftsvmmdgslkedaktpadydynvsvtaevvklahavgasvegelgclgsletgkgeae 155
                                       hqdhg+spa+c++ai+ gf+svmmdgsl+ed+k+ a+ydynv vt+evvk++ha+g+sve elg lgslet k+++e
  lcl|FitnessBrowser__PS:Dsui_0050  81 HQDHGQSPAVCMAAIKSGFSSVMMDGSLQEDGKSVASYDYNVAVTQEVVKFSHAIGVSVEAELGVLGSLETMKADKE 157
                                       ***************************************************************************** PP

                         TIGR01521 156 dghgfegaldrsqlltdpeeaaefvkktkvdalavaigtshgaykftrkptgevlaidrieeiherlpdthlvmhgs 232
                                       dghg+eg + r +lltdp++aa+fv++t+ dala+aigtshgaykft+kptg++laidri+eih+r+p+thlvmhgs
  lcl|FitnessBrowser__PS:Dsui_0050 158 DGHGAEGHMTREDLLTDPDQAADFVARTNCDALAIAIGTSHGAYKFTKKPTGDILAIDRIKEIHARIPNTHLVMHGS 234
                                       ***************************************************************************** PP

                         TIGR01521 233 ssvpqewldvineyggeiketygvpveeivkgikfgvrkvnidtdlrlaataalrrvaakdpsefdprkflkkavea 309
                                       ssvpq+ l+ i e+gg++ketygvpveeiv+gik+gvrkvnidtd rla+t+a+rr + ++ps+fdpr flk+a ea
  lcl|FitnessBrowser__PS:Dsui_0050 235 SSVPQDLLAEIREFGGDMKETYGVPVEEIVNGIKHGVRKVNIDTDIRLAMTGAVRRYLFENPSKFDPRDFLKPAREA 311
                                       ***************************************************************************** PP

                         TIGR01521 310 mkdvckaryeafgtagnaskikvvsleemarryakgel 347
                                        k++c+ary+afg  g a +ik++sle+ma+ry kgel
  lcl|FitnessBrowser__PS:Dsui_0050 312 AKKICVARYQAFGCEGRAGQIKPISLEKMAERYKKGEL 349
                                       ************************************97 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (347 nodes)
Target sequences:                          1  (354 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.07
# Mc/sec: 1.69
//
[ok]

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources