Aligments for a candidate for gadh2 in Dechlorosoma suillum PS

GapMind for catabolism of small carbon sources

Aligments for a candidate for gadh2 in Dechlorosoma suillum PS

Align D-gluconate dehydrogenase cytochrome c subunit (EC 1.1.99.3) (characterized)
to candidate Dsui_2086 Dsui_2086 cytochrome c

Query= metacyc::MONOMER-12746
         (434 letters)



>lcl|FitnessBrowser__PS:Dsui_2086 Dsui_2086 cytochrome c
          Length = 696

 Score =  386 bits (991), Expect = e-111
 Identities = 204/417 (48%), Positives = 269/417 (64%), Gaps = 19/417 (4%)

Query: 5   VIATLALLGSAAANAAEADQQALVQQGEYLARAGDCVACHTAKDGKPFAGGLPMETPIGV 64
           +++ LA  G   A AA+A Q   + +GEYLAR GDCVACHTA+ GK  AGG  + TP GV
Sbjct: 28  LLSALAAAGLGRAEAADAGQ---IARGEYLARLGDCVACHTAEGGKSMAGGRELATPFGV 84

Query: 65  IYSTNITPDK-TGIGDYSFEDFDKAVRHGVAKGGSTLYPAMPFPSYARVSDADMQALYAY 123
           ++STNITPDK TGIG+Y+F  FD+A+R GVA  G  LYPAMP+PSYA+++  DMQALYAY
Sbjct: 85  LFSTNITPDKETGIGNYTFAQFDRAMRKGVAADGHNLYPAMPYPSYAKMTGEDMQALYAY 144

Query: 124 FMKGVAPVARDNQDSDIPWPLSMRWPLSIWRWMFAPSVETP-APAAGSDPVISRGAYLVE 182
            M+G+APV + N+ + + WP + RW LS+W W F  +  TP  P AG D   +RGAYLV+
Sbjct: 145 LMQGLAPVKQANKPTAMRWPFNQRWGLSLWNWAFLDA--TPFQPDAGKDATWNRGAYLVQ 202

Query: 183 GLGHCGACHTPRALTMQEKALSASG--GSDFLSGSAPLEGWIAKSLRGDHKDGLGSWSEE 240
           GLGHCGACHTPR +  QEKA+S +G  G  FL+G   +E W A SLR       G W+ E
Sbjct: 203 GLGHCGACHTPRGIAFQEKAMSDAGSKGKHFLAGET-VESWRALSLR-------GLWTVE 254

Query: 241 QLVQFLKTGRSDRSAVFGGMSDVVVHSMQYMTDADLTAIARYLKSLPANDPK-DQPHQYD 299
               FLKTG++  +   G M++V+ HS Q++ D DL AIA YLKSLP  + +   P    
Sbjct: 255 DTALFLKTGQNRFATASGNMAEVIHHSTQHVKDEDLVAIATYLKSLPPGEHELPAPSVPR 314

Query: 300 KQVAQALWNGDDSKPGAAVYIDNCAACHRTDGHGYTRVFPALAGNPVLQSADATSLIHIV 359
            +VA  +     S  G   Y+  C  CHR DG+G +++FP LAGNP + + D  +L+HI 
Sbjct: 315 SEVAGKVPGNLFSSRGGLAYVQFCGDCHRQDGNGVSKIFPPLAGNPAVTAKDPATLLHIT 374

Query: 360 LKGGTLPATHSAPSTFTMPAFAWRLSDQEVADVVNFIRSSWGNQASAVKPGDVAALR 416
           L G    +T + P  FTMP FA RL+DQE+A+++ F+RSSWGN   AV    V  +R
Sbjct: 375 LTGWKTASTAAHPRVFTMPGFA-RLNDQELAELITFVRSSWGNGGEAVTASQVKKMR 430



 Score = 31.6 bits (70), Expect = 8e-05
 Identities = 35/140 (25%), Positives = 54/140 (38%), Gaps = 33/140 (23%)

Query: 274 ADLTAIARYLKSLP-ANDPKDQ-PHQYDKQVAQALWNGDDSKPGAAVYIDNCAACHRTDG 331
           AD+ A+  Y   +  A  P+D+ P +   ++ ++L    DS  G  +Y + CA CH  +G
Sbjct: 545 ADMKAMVAYFDWMKGATKPEDKVPGRGIGKIDRSL--KPDSDNGKKIYAEQCAVCHGENG 602

Query: 332 HGYTRVFPALAGNPVLQSADATSLIHIVLKGGTLPATHSAPSTFTMPAFAWR-------- 383
            G             L+SAD   +   +    +         T+T  AF  R        
Sbjct: 603 EG-------------LKSADGKMVYPPLWGDESFNIGAGMARTYTAAAFVKRNMPIGFHG 649

Query: 384 --------LSDQEVADVVNF 395
                   LSDQE  DV  +
Sbjct: 650 KFPLGQGGLSDQEALDVAEY 669


Lambda     K      H
   0.316    0.131    0.404 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 942
Number of extensions: 59
Number of successful extensions: 9
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 3
Number of HSP's successfully gapped: 2
Length of query: 434
Length of database: 696
Length adjustment: 36
Effective length of query: 398
Effective length of database: 660
Effective search space:   262680
Effective search space used:   262680
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources