Align predicted cytochrome c component of periplasmic glucoside 3-dehydrogenase (EC 1.1.99.13) (characterized)
to candidate Dsui_0927 Dsui_0927 cytochrome c551/c552
Query= reanno::Cola:Echvi_1841 (141 letters) >FitnessBrowser__PS:Dsui_0927 Length = 128 Score = 70.5 bits (171), Expect = 9e-18 Identities = 31/87 (35%), Positives = 55/87 (63%), Gaps = 1/87 (1%) Query: 53 DNPDYVEGLALVKESDCPSCHMVERKIVGPAYKDVAEKYESTDENIETLAKRVVDGNNGV 112 D+ +G + C CH V+ K +GP+Y+++A+KY T + + LAK++ DG G Sbjct: 32 DSGKLAKGAEIAVFRGCLGCHTVDSKRIGPSYQEIAKKYPHTPDVVAQLAKKIRDGGKGN 91 Query: 113 WGQVPMPAHPGLSEDDAKKMVKYILML 139 WG++ MPA+P +SE +A+ + +++L L Sbjct: 92 WGELSMPANP-VSEAEAQTLAEWVLSL 117 Lambda K H 0.309 0.127 0.361 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 48 Number of extensions: 1 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 141 Length of database: 128 Length adjustment: 15 Effective length of query: 126 Effective length of database: 113 Effective search space: 14238 Effective search space used: 14238 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.7 bits) S2: 42 (20.8 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory