Aligments for a candidate for livM in Dechlorosoma suillum PS

GapMind for catabolism of small carbon sources

Aligments for a candidate for livM in Dechlorosoma suillum PS

Align Transmembrane component of a broad range amino acid ABC transporter (characterized, see rationale)
to candidate Dsui_0628 Dsui_0628 ABC-type branched-chain amino acid transport system, permease component

Query= uniprot:Q1MCU1
         (463 letters)



>FitnessBrowser__PS:Dsui_0628
          Length = 358

 Score =  257 bits (657), Expect = 4e-73
 Identities = 160/333 (48%), Positives = 204/333 (61%), Gaps = 21/333 (6%)

Query: 113 IALLLYPMVVVAIKGPQGSLTYVDNFGIQILIYVMLAWGLNIVVGLAGLLDLGYVAFYAV 172
           I L + P +V A  G   +   + NF    ++Y+MLA GLNIVVG AGLLDLGY+AFYAV
Sbjct: 22  IVLAVLPFLVGA--GLGNAWLRILNFA---MLYIMLALGLNIVVGFAGLLDLGYIAFYAV 76

Query: 173 GAYSYALLSS-YFGLSF--WVLLPLSGIFAALWGVILGFPVLRLRGDYLAIVTLAFGEII 229
           GAY YALL+S +FGL +  W +LPL  + A   G +LG P LRLRGDYLAIVTL FGEII
Sbjct: 77  GAYLYALLASPHFGLHWPVWAILPLGAVVAGGAGALLGAPTLRLRGDYLAIVTLGFGEII 136

Query: 230 RLVLINWT---DVTKGTFGISSIPKATLFGIPFDATAGGFAKLFHLPISSAYYKIFLFYL 286
           R+ + N     ++T G  GISSI    + G+              +P   AYY     YL
Sbjct: 137 RIFMNNLNAPVNITNGPQGISSIDPFHVGGVTLAKPLSVLGVT--VPSLHAYY-----YL 189

Query: 287 ILALCMLTAYVTIRLRRMPIGRAWEALREDEIACRSLGINTVTTKLTAFATGAMFAGFAG 346
            L L ++  +VTIRL    IGRAW A+REDEIA ++ GIN    KL AF+ GA F G AG
Sbjct: 190 FLLLALVIIFVTIRLEDSRIGRAWVAIREDEIAAKACGINVRNIKLLAFSMGATFGGVAG 249

Query: 347 SFFAARQGFVSPESFVFLESAVILAIVVLGGMGSLTGIAIAAIVMVGGTELLRE--MSFL 404
             FA+ QGFVSPESF  +ES ++L +VVLGGMG + G+ +  I++    E  R   +   
Sbjct: 250 GLFASFQGFVSPESFGLMESIMVLCMVVLGGMGHIPGVILGGILLTILPEAFRHAAVPLQ 309

Query: 405 KLIFGPDFT-PELYRMLIFGLAMVVVMLFKPRG 436
           K  FG     PE  RML+FGLA++ VML++P G
Sbjct: 310 KYAFGKVVVDPESLRMLLFGLALIAVMLYRPAG 342


Lambda     K      H
   0.330    0.145    0.432 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 571
Number of extensions: 31
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 463
Length of database: 358
Length adjustment: 31
Effective length of query: 432
Effective length of database: 327
Effective search space:   141264
Effective search space used:   141264
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 50 (23.9 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources