GapMind for catabolism of small carbon sources

 

Protein 5210878 in Shewanella loihica PV-4

Annotation: FitnessBrowser__PV4:5210878

Length: 292 amino acids

Source: PV4 in FitnessBrowser

Candidate for 4 steps in catabolism of small carbon sources

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
L-citrulline catabolism PS417_17605 lo ATP-binding cassette domain-containing protein; SubName: Full=Amino acid transporter; SubName: Full=Histidine ABC transporter ATP-binding protein; SubName: Full=Histidine transport system ATP-binding protein (characterized, see rationale) 31% 95% 136.7 Intermembrane phospholipid transport system ATP-binding protein MlaF; EC 7.6.2.- 64% 351.7
glycerol catabolism glpT lo GlpT, component of Glycerol uptake porter, GlpSTPQV (characterized) 33% 59% 110.9 Intermembrane phospholipid transport system ATP-binding protein MlaF; EC 7.6.2.- 64% 351.7
D-alanine catabolism AZOBR_RS08245 lo Leucine/isoleucine/valine ABC transporter,ATPase component; EC 3.6.3.- (characterized, see rationale) 32% 91% 108.6 Intermembrane phospholipid transport system ATP-binding protein MlaF; EC 7.6.2.- 64% 351.7
L-proline catabolism AZOBR_RS08245 lo Leucine/isoleucine/valine ABC transporter,ATPase component; EC 3.6.3.- (characterized, see rationale) 32% 91% 108.6 Intermembrane phospholipid transport system ATP-binding protein MlaF; EC 7.6.2.- 64% 351.7

Sequence Analysis Tools

View 5210878 at FitnessBrowser

Find papers: PaperBLAST

Find functional residues: SitesBLAST

Search for conserved domains

Find the best match in UniProt

Compare to protein structures

Predict transmenbrane helices: Phobius

Predict protein localization: PSORTb

Find homologs in fast.genomics

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Sequence

MSHQSLSHDSQAKGETKPMEQAPMEHVQPLVEISHLGFSRGTHVIYDDVSLTIPRGKVTA
IMGPSGIGKTTLLKLIGGQLRPDSGVIKVDGVDVHKCNRSELFALRKRMSMLFQSGALFT
DMNVFDNVAFALREHSDLPEAIIRRIVLMKLEAVGLRGAAQMMPSELSGGMQRRAALARA
IALEPELVLYDEPFAGQDPISMGVLVKLIRELSDALSLTSVVVSHDVQEVLGIADYVYVL
ADKKVIAHGTPAELKAADNPKLKQFIGGEPDGPVPFHFPAQDYQKEMLGERD

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory