Align PP1070, component of Acidic amino acid uptake porter, AatJMQP (characterized)
to candidate 5210737 Shew_3165 polar amino acid ABC transporter, inner membrane subunit (RefSeq)
Query= TCDB::Q88NY3 (248 letters) >FitnessBrowser__PV4:5210737 Length = 226 Score = 97.1 bits (240), Expect = 3e-25 Identities = 72/232 (31%), Positives = 118/232 (50%), Gaps = 28/232 (12%) Query: 14 TGVGSETYLDWYITGLGWTIAIAITAWIIALLLGSLLGVMRTVPNR----LVSGIATAYV 69 T +G + + Y+ G + + +T + A++LG++LGV T+ + A YV Sbjct: 11 TPIGEDGLIGIYLILNGLKVTLIVT--LFAMILGAILGVATTLMKMSSRWYLRWPANLYV 68 Query: 70 ELFRNVPLLVQLFIWYFLVPDLLPEGLQEWFKQDLNPTTSALISVVICLGLFTAARVCEQ 129 + R P++VQL I YF+V L W D++ ++A+I+ GL + A + E Sbjct: 69 GVIRGTPVVVQLVILYFIV-------LATW---DVDKVSAAIIA----FGLNSGAYISEI 114 Query: 130 VRTGIQALPKGQEAAARAMGFSLPQIYNNVLLPQAYRIIIPPLTSEFLNVFKNSSVASLI 189 +R GIQA+ KGQ AAR++G S V+LPQA + I+P L +EF+ + K ++V I Sbjct: 115 IRAGIQAVDKGQTEAARSLGLSQAVTMKEVILPQAIKNILPALGNEFIVLLKETAVIGFI 174 Query: 190 GLMELLAQ----TKQTAEFSANLFEAFTLATLIYFTLNMGLMLLMRMVEKKV 237 G ++L+ +T E S LF LIY L ++ EK++ Sbjct: 175 GGVDLMRSGEIIRSRTFEDSVPLF----TCALIYLALTYSFTFMLSKFEKRL 222 Lambda K H 0.325 0.139 0.425 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 120 Number of extensions: 5 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 248 Length of database: 226 Length adjustment: 23 Effective length of query: 225 Effective length of database: 203 Effective search space: 45675 Effective search space used: 45675 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 46 (22.3 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory