Alignments for a candidate for agcS in Shewanella loihica PV-4

GapMind for catabolism of small carbon sources

Alignments for a candidate for agcS in Shewanella loihica PV-4

Align Probable Glycine/alanine/asparagine/glutamine uptake porter, AgcS (characterized)
to candidate 5207980 Shew_0496 amino acid carrier protein (RefSeq)

Query= TCDB::W0WFC6
         (449 letters)



>FitnessBrowser__PV4:5207980
          Length = 457

 Score =  447 bits (1149), Expect = e-130
 Identities = 231/442 (52%), Positives = 302/442 (68%), Gaps = 12/442 (2%)

Query: 10  DLNGVVWGPLMLVLILGTGLYLMLGLKFMPLVRLGVGFRLLWQGRSKDDESSGEISPFQA 69
           ++N +VWGP+ L L++GTGLYL L L+ + + +L +  RLL++  S      G++S F A
Sbjct: 9   EINAIVWGPITLCLLVGTGLYLTLRLRLIQVFKLPLALRLLFKPAS----GQGDLSSFAA 64

Query: 70  LMTCLAATVGTGNIAGVATAIFLGGPGALFWMWCTALVGMATKFSEVVLAVHYREKDERN 129
           L T L+AT+GTGNI GVATAI +GGPGALFWMW  A  GMATK+ E +LA+ YR  D R 
Sbjct: 65  LCTALSATIGTGNIVGVATAIKVGGPGALFWMWLAAFFGMATKYGECMLALKYRTTDARG 124

Query: 130 EHVGGPMYAIKNGLGKRWAWLGAAFALFG-GLAGFGIGNMVQVNSMADALEVSFGVPDWV 188
              GGPMY I+ GLG    WL   FALFG G+A FGIG   QVN+++DA+ ++F VP WV
Sbjct: 125 NIAGGPMYYIERGLGL--GWLAKLFALFGVGVAFFGIGTFAQVNAISDAMTIAFHVPAWV 182

Query: 189 TGVATMLVTGLVILGGIRRIGKVAEALVPFMCVGYIVASVIVLVVHAEAIPGAFQLIFTH 248
           T +   L+   V LGG++RI KVA+ LVP M +GY++A + +LV  +E I  A QL+   
Sbjct: 183 TALVLTLLVAAVTLGGVKRIAKVAQKLVPSMALGYVLACLWILVTFSEQIIPALQLVIHS 242

Query: 249 AFTPIAATGGFAGAAVMAAIRFGVARGIFSNEAGLGTAGIAQAAGTTHSAVRSGLIGMLG 308
           AF+PI+A GGF GA V  AI+ G+ARG+FSNE+GLG+A IA AA  T   V  GL+ M G
Sbjct: 243 AFSPISAAGGFLGATVAQAIQIGIARGVFSNESGLGSAPIAAAAAKTKEPVEQGLVSMTG 302

Query: 309 TFIDTLIICSLTGLAIITSGVWTSGASGAALSSAAFEAAMPGV-GHYILSLALVVFAYTT 367
           TF DTL+IC++TGL +I +GVW+  A+GAA++SAAF      V G Y++++ALV FA+TT
Sbjct: 303 TFFDTLLICTITGLVLIITGVWSGDAAGAAMTSAAFATGGSAVIGQYLVTIALVCFAFTT 362

Query: 368 ILGWSYYGERCWEYLA----GTRAILPFRIVWTLAIPFGAMTQLDFAWLVADTLNALMAI 423
           ILGW YYGERCW YL     G R I  ++ ++   I  GA  QLD  WL+ADT+N LMAI
Sbjct: 363 ILGWHYYGERCWYYLTGHKLGERGIKIYQFIFLSLIAVGAFIQLDLIWLLADTVNGLMAI 422

Query: 424 PNLIALLLLSPVVFRLTREYFA 445
           PNLIAL+ L  V+   T  YFA
Sbjct: 423 PNLIALIGLRKVIIADTLSYFA 444


Lambda     K      H
   0.326    0.140    0.437 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 617
Number of extensions: 31
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 449
Length of database: 457
Length adjustment: 33
Effective length of query: 416
Effective length of database: 424
Effective search space:   176384
Effective search space used:   176384
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources