Aligments for a candidate for dctA in Shewanella loihica PV-4

GapMind for catabolism of small carbon sources

Aligments for a candidate for dctA in Shewanella loihica PV-4

Align The dicarboxylate (succinate, fumarate, malate and oxaloacetate):H+ symporter, DctA (probably 3H+ are transported per succinate taken up (characterized)
to candidate 5209774 Shew_2227 sodium:dicarboxylate symporter (RefSeq)

Query= TCDB::P96603
         (421 letters)



>lcl|FitnessBrowser__PV4:5209774 Shew_2227 sodium:dicarboxylate
           symporter (RefSeq)
          Length = 439

 Score =  234 bits (598), Expect = 3e-66
 Identities = 130/419 (31%), Positives = 232/419 (55%), Gaps = 34/419 (8%)

Query: 7   LTVQVITAVIIGVIVGLVWPDV--GKE----------MKPLGDTFINAVKMVIAPIIFFT 54
           LT +++  +  G++ GL+      G E          +  +G  FI++++M++ P++F +
Sbjct: 11  LTGKILIGMAGGILTGLLLRSFFPGSEFIEEYITNGLLNVVGSIFISSLQMLVVPLVFIS 70

Query: 55  IVLGIAKMGDMKKVGKVGGKAFIYFEVVTTLALIIGLFVVNIMKPG------AGLDYSKL 108
           +V G   + D   +G++GGK   ++   T +AL + + V  ++ PG        + YS  
Sbjct: 71  LVCGTCSLSDPSSLGRLGGKTIAFYLFTTAIALSMAILVALLVHPGNASLAAENMQYSVK 130

Query: 109 EKGDVSQYTQNGGQGIDWIEFITHIVPSNMVDAFAKGDILQVLFFSILFGVGLAALGEKG 168
           E   +S             + + ++VP N + A  +G++LQ++ F+++FG  ++ +GE+G
Sbjct: 131 EAPSLS-------------DVLINLVPRNPIQAMTEGNMLQIIIFAVIFGFAISHIGERG 177

Query: 169 KSVIDFFDKVSHVFFKIIGYIMRAAPIGAFGAMAYTIGHFGLDSIKPLASLMMSVYITMF 228
           K V   F+ ++ V  +++  IM+ AP G F  M       GL++ + +    M V   + 
Sbjct: 178 KRVSALFNDLNEVIMRVVTLIMQLAPYGIFALMGKLALTLGLETFESVVKYFMVVLAVLL 237

Query: 229 LFVFVALNIICKLY-GFSLWNYLRFIKDELLIVLGTSSSESVLPRMMDKME-RYGCSKSV 286
           +  FV+  ++ KL+ G S + ++R ++D  L    T+SS + LP  ++  E R G    V
Sbjct: 238 VHAFVSYPVLLKLFSGLSPFTFIRKMRDVQLFAFSTASSNATLPVTLETSEHRLGVDNKV 297

Query: 287 VGLVIPTGYSFNLDGTSIYLSMATVFLAQVFGVDLSIGQQITIILVLMLTSKGAAGVTGS 346
               +P G + N+DGT+I   +ATVF+AQVFG+DL+I     +++   L S G AGV G 
Sbjct: 298 ASFTLPLGATINMDGTAIMQGVATVFIAQVFGIDLTITDYAMVVVTATLASIGTAGVPGV 357

Query: 347 GFIVLASTLSALQVIPLEGLALLLGVDRFMSEGRAIVNLIGNGIATIIVAKSENEFDEA 405
           G I+LA  L+ +  +P+EG+AL++GVDR +   R  VN+ G+ +AT+I+AKSEN F++A
Sbjct: 358 GLIMLAMVLNQVG-LPVEGIALIMGVDRLLDMVRTAVNVTGDSVATVIIAKSENAFNQA 415


Lambda     K      H
   0.326    0.143    0.408 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 402
Number of extensions: 17
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 421
Length of database: 439
Length adjustment: 32
Effective length of query: 389
Effective length of database: 407
Effective search space:   158323
Effective search space used:   158323
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources