Align fumarate hydratase (EC 4.2.1.2); (S)-2-methylmalate dehydratase (EC 4.2.1.34) (characterized)
to candidate 5209433 Shew_1904 tartrate/fumarate subfamily Fe-S type hydro-lyase alpha subunit (RefSeq)
Query= BRENDA::Q141Z6 (520 letters) >FitnessBrowser__PV4:5209433 Length = 507 Score = 717 bits (1850), Expect = 0.0 Identities = 347/507 (68%), Positives = 413/507 (81%), Gaps = 3/507 (0%) Query: 8 VSTELAMTVIKQEDLIQSIADSLQYISYYHPLDYIQALGRAYELEQSPAAKDAIAQILTN 67 +S + +IKQ DLI+S+AD+LQYISYYHP D++ A+ AYE E+S AAKDAIAQIL N Sbjct: 1 MSVTIEQPIIKQADLIESVADALQYISYYHPKDFVDAMAEAYEREESAAAKDAIAQILIN 60 Query: 68 SRMCAEGKRPICQDTGIVTVFVKVGMDVRWDGATMGVTDMINEGVRRGYLNPDNVLRASI 127 SRM AEGKRP+CQDTGIVT FVK+GM V+WD M V M++EGVRR Y NPDN LRASI Sbjct: 61 SRMSAEGKRPLCQDTGIVTCFVKIGMGVKWDKTDMTVQQMVDEGVRRAYTNPDNPLRASI 120 Query: 128 VSPPEGGRKNTKDNTPAVIHYEIVPGNTVDVQVAAKGGGSENKSKFAMLNPSDSIVDWIL 187 V+ P GGRKNTKDNTP+V+H E+VPGN V+V +AAKGGGSENKSK MLNPSD I +W+ Sbjct: 121 VADPAGGRKNTKDNTPSVVHVEMVPGNHVEVAIAAKGGGSENKSKMVMLNPSDDIAEWVE 180 Query: 188 KTVPTMGAGWCPPGMLGIGIGGTAEKAMVMAKESLMDPIDIQDVIARGPKDWIEELRVEL 247 KT+PTMGAGWCPPGMLGIGIGGTAEKA VMAKE+LMDP+DI ++ A+G + E+LR+++ Sbjct: 181 KTLPTMGAGWCPPGMLGIGIGGTAEKAAVMAKEALMDPVDIHELQAKGAETAEEKLRLDI 240 Query: 248 HEKVNALGIGAQGLGGLATVLDVKIMAAPTHAASKPVAIIPNCAATRHAHFTLDGSGAAR 307 E+ N LGIGAQGLGGL TVLD+KI +APTHAASKPV +IPNCAATRH HF LDGSG A Sbjct: 241 FERANKLGIGAQGLGGLTTVLDIKIKSAPTHAASKPVVMIPNCAATRHVHFHLDGSGPAE 300 Query: 308 LEAPSLDAWPKVHWQPDTEKSKRVDLNTLTPEEVAAWTPGQTLLLSGKMLTGRDAAHKRI 367 LE P L+ WP++ + E +RVDLNT+T ++ W G LLL+GKMLTGRDAAHKRI Sbjct: 301 LETPKLEDWPEITREAG-EGVRRVDLNTVTQADIEQWKSGDVLLLNGKMLTGRDAAHKRI 359 Query: 368 ADMLAKGEKLP--VDFTNRVIYYVGPVDPVRDEAVGPAGPTTATRMDKFTEMMLAQTGLI 425 ++ GE LP VDFT + IYYVGPVDPV DE VGPAGPTTATRMDKFT++ML +TGL+ Sbjct: 360 QTLIESGEGLPEGVDFTGKFIYYVGPVDPVGDEVVGPAGPTTATRMDKFTDLMLDKTGLM 419 Query: 426 SMIGKAERGPVAIEAIRKHKAAYLMAVGGAAYLVSKAIRSAKVLAFEDLGMEAIYEFDVQ 485 MIGKAERGP +E+I+KHKA YLMAVGGAAYLVSKAI+ ++V+AFEDLGMEAIYEFDV Sbjct: 420 GMIGKAERGPATVESIKKHKAVYLMAVGGAAYLVSKAIKKSRVVAFEDLGMEAIYEFDVT 479 Query: 486 DMPVTVAVDSNGTSVHQTGPKEWQARI 512 DMPVTVAVDS G + H+TGP W+ ++ Sbjct: 480 DMPVTVAVDSEGVNAHETGPATWRVKL 506 Lambda K H 0.316 0.133 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 886 Number of extensions: 33 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 520 Length of database: 507 Length adjustment: 35 Effective length of query: 485 Effective length of database: 472 Effective search space: 228920 Effective search space used: 228920 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 52 (24.6 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory