GapMind for catabolism of small carbon sources

 

Alignments for a candidate for gltP in Shewanella loihica PV-4

Align Sodium:dicarboxylate symporter (characterized, see rationale)
to candidate 5209774 Shew_2227 sodium:dicarboxylate symporter (RefSeq)

Query= uniprot:A1S570
         (437 letters)



>FitnessBrowser__PV4:5209774
          Length = 439

 Score =  703 bits (1815), Expect = 0.0
 Identities = 359/434 (82%), Positives = 402/434 (92%)

Query: 1   MAAAQSSKIGLTGKILIGMGAGILIGLLLRNFFGGSEWVQDYITEGFFHVIGTIFINSLK 60
           MA  Q+ K+GLTGKILIGM  GIL GLLLR+FF GSE++++YIT G  +V+G+IFI+SL+
Sbjct: 1   MAGTQNKKLGLTGKILIGMAGGILTGLLLRSFFPGSEFIEEYITNGLLNVVGSIFISSLQ 60

Query: 61  MLVVPLVFISLVCGTCSLSEPSKLGRLGGKTLAFYLFTTAIALVVAISAAVLVQPGNASL 120
           MLVVPLVFISLVCGTCSLS+PS LGRLGGKT+AFYLFTTAIAL +AI  A+LV PGNASL
Sbjct: 61  MLVVPLVFISLVCGTCSLSDPSSLGRLGGKTIAFYLFTTAIALSMAILVALLVHPGNASL 120

Query: 121 ASESMQYSAKEAPSLADVLINIVPSNPMKALSEGNMLQIIIFAVIFGFAISHIGERGRRV 180
           A+E+MQYS KEAPSL+DVLIN+VP NP++A++EGNMLQIIIFAVIFGFAISHIGERG+RV
Sbjct: 121 AAENMQYSVKEAPSLSDVLINLVPRNPIQAMTEGNMLQIIIFAVIFGFAISHIGERGKRV 180

Query: 181 AALFDDLNEVIMRVVTLIMQLAPYGVFALMGKLALTLGMETLESVIKYFMLVLVVLLFHG 240
           +ALF+DLNEVIMRVVTLIMQLAPYG+FALMGKLALTLG+ET ESV+KYFM+VL VLL H 
Sbjct: 181 SALFNDLNEVIMRVVTLIMQLAPYGIFALMGKLALTLGLETFESVVKYFMVVLAVLLVHA 240

Query: 241 FVVYPTLLKLFSGLSPLMFIRKMRDVQLFAFSTASSNATLPVTMEASEHRLGADNKVASF 300
           FV YP LLKLFSGLSP  FIRKMRDVQLFAFSTASSNATLPVT+E SEHRLG DNKVASF
Sbjct: 241 FVSYPVLLKLFSGLSPFTFIRKMRDVQLFAFSTASSNATLPVTLETSEHRLGVDNKVASF 300

Query: 301 TLPLGATINMDGTAIMQGVATVFIAQVFGIDLTITDYAMVVMTATLASIGTAGVPGVGLV 360
           TLPLGATINMDGTAIMQGVATVFIAQVFGIDLTITDYAMVV+TATLASIGTAGVPGVGL+
Sbjct: 301 TLPLGATINMDGTAIMQGVATVFIAQVFGIDLTITDYAMVVVTATLASIGTAGVPGVGLI 360

Query: 361 MLAMVLNQVGLPVEGIALILGVDRMLDMVRTAVNVTGDTVATVVIAKSEGALNEAVFNDP 420
           MLAMVLNQVGLPVEGIALI+GVDR+LDMVRTAVNVTGD+VATV+IAKSE A N+A F+DP
Sbjct: 361 MLAMVLNQVGLPVEGIALIMGVDRLLDMVRTAVNVTGDSVATVIIAKSENAFNQATFDDP 420

Query: 421 KAGKTAGSFDAEVH 434
           +AGKTAGSF  +V+
Sbjct: 421 QAGKTAGSFVDQVN 434


Lambda     K      H
   0.325    0.139    0.388 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 580
Number of extensions: 16
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 437
Length of database: 439
Length adjustment: 32
Effective length of query: 405
Effective length of database: 407
Effective search space:   164835
Effective search space used:   164835
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory