Alignments for a candidate for lacL in Shewanella loihica PV-4

GapMind for catabolism of small carbon sources

Alignments for a candidate for lacL in Shewanella loihica PV-4

Align β-galactosidase (LacL+LacM) (EC 3.2.1.23) (characterized)
to candidate 5210843 Shew_3269 Beta-galactosidase (RefSeq)

Query= CAZy::CAZ66936.1
         (626 letters)



>FitnessBrowser__PV4:5210843
          Length = 1076

 Score =  347 bits (891), Expect = 1e-99
 Identities = 216/627 (34%), Positives = 322/627 (51%), Gaps = 44/627 (7%)

Query: 10  DPEVFRVNQLPAHSDHHYYHDTA----EFKTGSRFIKSLNGAWRFNFAKTPAERPVDFYQ 65
           D  +F VN+L  H+    Y        +    S+    LNG WRF+ AK P   P +F  
Sbjct: 37  DHTLFEVNKLAPHASFFGYESEPLALLDEMDRSQLYLDLNGRWRFHLAKNPDATPKEFAA 96

Query: 66  PDFDATDFDTIQVPGHIELAGYGQIQYINTLYPWEGKIYRRPPYTLNQDQLTPGLFSDAA 125
           P+FDA+ + +IQVPG+ E  GYG   Y++  YP++ K               P   SD  
Sbjct: 97  PEFDASHWGSIQVPGNFETQGYGHAIYLDERYPFDTK--------------WPDAPSD-- 140

Query: 126 DNTVGSYLKTFDLDDAFKGQRIIIQFQGVEEALYVWLNGHFIGYAEDSFTPSEFDLTPYI 185
            N  G Y KTF L   ++ +++ I       AL +++NG  +GY++ + TP+EFD+TPY+
Sbjct: 141 HNPTGLYRKTFTLPAHWQQKQVFIHIGAARSALTLFVNGREVGYSQGAKTPAEFDITPYL 200

Query: 186 QDQGNVLAVRVYKRSTAAFIEDQDMFRFSGIFRDVNILAEPASHITDLDIRPVPNANLKS 245
           Q   N++A+++ + S A+++E QDM R +GI R+V + A P   I D+ +    N +L  
Sbjct: 201 QAGDNLVAMQLIRWSDASYLESQDMLRMTGIEREVYLYATPKQRIEDIQVVTHLNEDLTR 260

Query: 246 GELNITTKVTG-EPATLALTVK----DHDGRVLTSQTQ----TGSGSVTFDTMLFDQLHL 296
            +L I   +   +P   AL ++    D  G+ +    Q     G     F   L     L
Sbjct: 261 AKLAIRVDIASHQPGVRALELEARLLDPQGKPVAKANQRLSLKGDAKPVFSQTLISP-KL 319

Query: 297 WSPQTPYLYQLTIEVYDADRQLLEVVPYQFGFRTVELRDDKVIYVNNKRLVINGVNRHEW 356
           W+ + P LY+L + +     + L+V   Q G R + + + + + VNNK + I GV+RHE 
Sbjct: 320 WNAEMPNLYRLILTLKTEKGETLQVASQQIGVRKIAIENGQ-LKVNNKAITIRGVDRHET 378

Query: 357 NAHTGRVISMDDMRADIQTMLANNINADRTCHYPDQLPWYQLCDEAGIYLMAENNLESHG 416
           +  TG V+S + M  DI+ M  NNINA R+ HYP+   W  L D  G+Y++ E N+ESH 
Sbjct: 379 DPQTGHVVSRETMELDIRLMKQNNINAVRSSHYPNHPYWLSLADRYGLYVIDEANIESHP 438

Query: 417 SWQKMGAIEPSYNVPGDNPHWLAAVIDRARSNYEWFKNHPSIIFWSLGNESYAGEDIAAM 476
                 AI+    + G+   WL A   R     E  KNHPS+I WSLGNE+  G+    +
Sbjct: 439 L-----AIDDKTQL-GNEMSWLPAHQARIERMVERDKNHPSVIIWSLGNEAGEGKLFERL 492

Query: 477 QAFYKEHDDSRLVHYEGVVHTPELKDRISDVESRMYEKPQNIVAYLEDNPTKPFLDCEYM 536
             + K  D +R V YE     P      +D+ + MY   + I  Y E    +P +  EY 
Sbjct: 493 YQWIKRRDPNRPVQYEPAGEAP-----YTDIVAPMYPSIERIREYAERASDRPLIMIEYA 547

Query: 537 HDMGNSLGGMQSYNDLIDKYPMYQGGFIWDFIDQALFVHDPITDQDVLRYGGDF-DERHS 595
           H MGNS+G +Q Y D+I+ YP  QGGFIWD++DQAL   + +  Q    YG D+  +  +
Sbjct: 548 HAMGNSVGNLQDYWDVIEAYPQLQGGFIWDWVDQALAFSNDL-GQRYWAYGKDYHPDMPT 606

Query: 596 DYEFSGDGLMFADRTPKPAMQEVKYYY 622
           D  F  +GL+  DR P P + EVK  Y
Sbjct: 607 DGNFLNNGLVDPDRNPHPHLSEVKKVY 633


Lambda     K      H
   0.320    0.138    0.429 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1812
Number of extensions: 95
Number of successful extensions: 7
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 626
Length of database: 1076
Length adjustment: 41
Effective length of query: 585
Effective length of database: 1035
Effective search space:   605475
Effective search space used:   605475
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 56 (26.2 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources