GapMind for catabolism of small carbon sources

 

Aligments for a candidate for liuB in Shewanella loihica PV-4

Align Methylcrotonoyl-CoA carboxylase (EC 6.4.1.4) (characterized)
to candidate 5210939 Shew_3365 acetyl-CoA carboxylase, biotin carboxylase (RefSeq)

Query= reanno::pseudo5_N2C3_1:AO356_01595
         (649 letters)



>lcl|FitnessBrowser__PV4:5210939 Shew_3365 acetyl-CoA carboxylase,
           biotin carboxylase (RefSeq)
          Length = 450

 Score =  428 bits (1100), Expect = e-124
 Identities = 225/450 (50%), Positives = 308/450 (68%), Gaps = 17/450 (3%)

Query: 9   LLVANRGEIACRVMRTARAMGLTTVAVHSATDRDARHSREADIRVDLGGSKAADSYLQID 68
           +LVANRGEIA R++R  R +G+ TVA++S+ D+D+ H R AD ++ +G + A +SYL I 
Sbjct: 7   VLVANRGEIALRIIRACRQLGIKTVALYSSADKDSGHLRLADQQICIGPAPAKESYLNIA 66

Query: 69  KLIAAAKASGAQAIHPGYGFLSENAGFARAIENAGLIFLGPPASAIDAMGSKSAAKTLME 128
            ++ AA  +   AIHPGYGFL+ENA FA+ + ++G  F+GP A  I  MG K +A   M+
Sbjct: 67  AILGAADLARVDAIHPGYGFLAENADFAQQVTDSGFHFIGPKADTIRLMGDKVSAIAAMK 126

Query: 129 TAGVPLVPGYHG-----EAQDLETFRDAAERIGYPVLLKATAGGGGKGMKVVEDVSQLAE 183
            AGVP VPG  G     EAQ LE     A +IGYP+++KATAGGGGKGM+VVED +QL  
Sbjct: 127 AAGVPTVPGSDGLLGDNEAQSLEI----AAQIGYPLIIKATAGGGGKGMRVVEDEAQLLG 182

Query: 184 ALASAQREAQSSFGDSRMLVEKYLLKPRHVEIQVFADQHGNCLYLNERDCSIQRRHQKVV 243
           A+A  ++EA+++FG+  + +EKYL +PRH+E+QV  D  G+C+ L ERDCS+QR  QKV+
Sbjct: 183 AIALTRQEAEAAFGNGGVYLEKYLTRPRHIEVQVLCDHQGHCISLGERDCSLQRAQQKVI 242

Query: 244 EEAPAPGLTPQLRRAMGEAAVRAAQAIGYVGAGTVEFLLDARGEFFFMEMNTRLQVEHPV 303
           E APAPGLTP+ R+ + E+ +RA QAIGY GAGT+EFL    GEF+FMEMNTR+QVEH +
Sbjct: 243 ESAPAPGLTPEQRQQISESCIRACQAIGYRGAGTLEFLYQ-DGEFYFMEMNTRIQVEHTI 301

Query: 304 TEAITGLDLVAWQIRVAQGEPLPITQAQVPLLGHAIEVRLYAEDPGNDFLPATGRLALYR 363
           +E +TG+DL+  Q+RVAQGEPL + ++ +   GHAIE R+ AEDP + F P+ GR+    
Sbjct: 302 SEMVTGVDLLVLQLRVAQGEPLALQESLIQPKGHAIECRINAEDPAS-FAPSPGRIDRLV 360

Query: 364 ESAEGPGRRVDSGVEEGDEISPFYDPMLGKLIAWGEDREQARLRLLSMLDEFVIGGLKTN 423
               G G R +S +  G  + P YD M+ KLIAWG+DR QA  R+ + LDE  I G+KTN
Sbjct: 361 VPG-GLGVRWESHLSPGAHVPPQYDAMIAKLIAWGDDRAQAIARMRTALDELTITGIKTN 419

Query: 424 IGFLRRIVAHPAFAAAE-----LDTGFIPR 448
           +  LR ++A P     E     L+  F+PR
Sbjct: 420 VPLLRALLADPQVQQGETSIHYLEETFLPR 449


Lambda     K      H
   0.319    0.134    0.390 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 761
Number of extensions: 31
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 649
Length of database: 450
Length adjustment: 35
Effective length of query: 614
Effective length of database: 415
Effective search space:   254810
Effective search space used:   254810
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory