Align α-glucosidase (SusB;Aglu;BFO_2272) (EC 3.2.1.20) (characterized)
to candidate 5209405 Shew_1876 alpha-glucosidase (RefSeq)
Query= CAZy::AEW20125.1 (708 letters) >FitnessBrowser__PV4:5209405 Length = 707 Score = 505 bits (1300), Expect = e-147 Identities = 286/695 (41%), Positives = 402/695 (57%), Gaps = 67/695 (9%) Query: 29 LKSPDGNLLMRFEVNGEGTPVYELTYKRKPVIKPSRLGLEIEGFS-LRKAFTLKGVETVT 87 L SPD +++ + + TP Y +++ K VI+PS+LG+ E F+++ V+ Sbjct: 54 LTSPDKQIVVSLS-DDDQTPRYSVSFHGKKVIEPSKLGIVFESLGEFGTDFSIQEVKRSQ 112 Query: 88 FDETWTPVWGEVKAIRNHYNEMTATLHQADGDRTMVIRFRLYDDGLGFRYEFPQQKSLNY 147 DE W WGE + + + +N +TATL ++G V+ F+++DDGLGFRY+ P+Q L+ Sbjct: 113 ADERWQQPWGEREWVVDKHNGLTATL--SNGQYRFVVEFKVFDDGLGFRYQMPKQGDLDK 170 Query: 148 FVIKEEYSEFAMAGDHK--AFWIPG-DYDTQEYDYTESRLTEIRGLMQTAITPNTSQTPF 204 I E +EF + A+WIP ++ EY Y ++ L ++ + TPF Sbjct: 171 LNITSELTEFTLPQPENVTAWWIPARGWNRYEYLYQKTPLKQV----------DRVHTPF 220 Query: 205 SPTGVQTALMMKSDDGLYINLHEAALVDYPCMSLNLDDQTLTFQSWLTPDAQGKKGYMQT 264 + T DG+++++HEAALVDY M+L+ + T ++ LTP + G + Q Sbjct: 221 TFT---------LSDGVHMSIHEAALVDYAAMTLD-QRRDGTLRADLTPWSDGMRVKTQA 270 Query: 265 PCHTPWRTVIVSDDARRILASHLTLNLNEPCKLDDVSWIRPVKYIGVWWEMISGKSTWAY 324 TPWRT+ ++D A +L S L LNLNEP KL DV W+ P KY+G+WW M ++TW Sbjct: 271 GFSTPWRTIQIADKATGLLNSDLILNLNEPNKLGDVDWVEPGKYVGIWWGMHLNENTWG- 329 Query: 325 TNDLPSVHLDVTDYTKMKPNGTHAANNEKVRRYIDFAAKHGFDQVLVEGWNIGWE-DWFG 383 H A + +RY+DFAA++GF VLVEGWNIGW+ WF Sbjct: 330 ------------------SGDKHGATTSETKRYMDFAAENGFAGVLVEGWNIGWDGSWFH 371 Query: 384 NSKDYVFDFVTPYPDFDLKALNEYAHSKGVKLMMHHETSSSVRNYERQMEKAYKLMNDYG 443 N VF F YPDFDL ++ Y SKGV+L+ HHETS SV NY +QM AY L YG Sbjct: 372 NGD--VFSFTQAYPDFDLPEISAYGASKGVRLIGHHETSGSVTNYRKQMSDAYDLYQQYG 429 Query: 444 YNAVKSGYV---GNI-------IPRGEYHYGQWMVNHYLYAVKKAAEHRIMVNAHEAVRP 493 VK+GYV G+I I R E+H GQ+MVN YL++V +AA+ I +N HE ++ Sbjct: 430 VTQVKTGYVADGGDIKRVDEQGIVRHEWHDGQFMVNEYLHSVTEAAKRHISINTHEPIKD 489 Query: 494 TGLCRTYPNLIGNEAARGGEYESFGG--NKVFHTTILPFTRLMGGPMDYTPGIFETRLEK 551 TGL RTYPN I E ARG E+ ++G N HTT L +TR++ GPMD+TPGIF+ + Sbjct: 490 TGLRRTYPNWIAREGARGQEFNAWGTPPNNPSHTTTLAYTRMLAGPMDFTPGIFDLAPKG 549 Query: 552 VNPGNNSYVHSTLARQLALYVTMYSPLQMAADLPENYERFMDAFQFIKDVAIDWDDSKYL 611 ++ N V +TL +QLALYV +YSP+QMAADLP NY++ DAF+FI+DV DW S L Sbjct: 550 LDAVNR--VQTTLTKQLALYVVLYSPIQMAADLPRNYKQHPDAFKFIRDVPTDWAQSIAL 607 Query: 612 EAEPGRYITVARKAKG----TNNWFVGCTANEDGHLSNVSLDFLDPGRKYTATIYADAPT 667 E G Y+ AR+ +G +W++G ++D N+ LDFLDP Y IY D Sbjct: 608 AGEVGEYVAFARQERGGKSQGKDWYLGAITDDDAREINIKLDFLDPALSYEVQIYRDGDK 667 Query: 668 AHYEKNPQAYTIKQMKVTNKSKLTLRAASGGGYAI 702 A + NP Y I+ KV +K +LTL+ AS GG AI Sbjct: 668 ADWRTNPYDYVIETQKVNSKQQLTLKLASSGGVAI 702 Lambda K H 0.319 0.135 0.417 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1469 Number of extensions: 84 Number of successful extensions: 11 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 708 Length of database: 707 Length adjustment: 39 Effective length of query: 669 Effective length of database: 668 Effective search space: 446892 Effective search space used: 446892 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 55 (25.8 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory