Align Maleylacetoacetate isomerase (EC 5.2.1.2) (characterized)
to candidate 5208938 Shew_1439 maleylacetoacetate isomerase (RefSeq)
Query= reanno::MR1:200836 (216 letters) >FitnessBrowser__PV4:5208938 Length = 214 Score = 298 bits (764), Expect = 4e-86 Identities = 145/215 (67%), Positives = 172/215 (80%), Gaps = 2/215 (0%) Query: 1 MILYGYWRSSAAYRVRIALNLKGVSAEQLSVHLVRDGGEQHKADYIALNPQELVPTLVVD 60 M L GYWRSSAAYRVRIALNLKG+ AE SVHLV++GGEQH +Y ALNPQELVPTL+ Sbjct: 1 MKLLGYWRSSAAYRVRIALNLKGLDAELESVHLVKNGGEQHLPEYAALNPQELVPTLIEG 60 Query: 61 DEQDGDALTQSLAIIEYLDELYPKTPLLPASALERAHVRAMALTIACEIHPLNNLRVLQY 120 D + L+QSLAIIEYLDE Y +LP + RA VRAMAL+IACE+HPLNNL+VLQY Sbjct: 61 DNEF--VLSQSLAIIEYLDEQYGGAMMLPTAPKARAEVRAMALSIACEVHPLNNLKVLQY 118 Query: 121 LTQKLTVNEEAKSAWYHHWVATGFTALETQLVRHSGRYCFGDKVTIADLCLVPQVYNAQR 180 LT L ++E+AK+AWYHHW+ GF+A E QL +H+G YC+GD+VT+ADLCL+PQVYNA R Sbjct: 119 LTNTLELDEDAKNAWYHHWIHQGFSAFEKQLEKHAGSYCYGDEVTLADLCLIPQVYNANR 178 Query: 181 FNVDLTPYPNIMRVWAECNQLPAFADAAPERQADA 215 F VDL+ YPNI R+W C+QL AF AAPERQADA Sbjct: 179 FKVDLSTYPNIRRIWDNCHQLEAFVLAAPERQADA 213 Lambda K H 0.321 0.134 0.408 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 183 Number of extensions: 3 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 216 Length of database: 214 Length adjustment: 22 Effective length of query: 194 Effective length of database: 192 Effective search space: 37248 Effective search space used: 37248 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 45 (21.9 bits)
Align candidate 5208938 Shew_1439 (maleylacetoacetate isomerase (RefSeq))
to HMM TIGR01262 (maiA: maleylacetoacetate isomerase (EC 5.2.1.2))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.carbon/TIGR01262.hmm # target sequence database: /tmp/gapView.24607.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR01262 [M=211] Accession: TIGR01262 Description: maiA: maleylacetoacetate isomerase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 1.4e-91 291.9 0.1 1.5e-91 291.7 0.1 1.0 1 lcl|FitnessBrowser__PV4:5208938 Shew_1439 maleylacetoacetate iso Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__PV4:5208938 Shew_1439 maleylacetoacetate isomerase (RefSeq) # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 291.7 0.1 1.5e-91 1.5e-91 2 210 .. 3 213 .. 2 214 .] 0.98 Alignments for each domain: == domain 1 score: 291.7 bits; conditional E-value: 1.5e-91 TIGR01262 2 lYsyfrSsasyRvRiaLaLkgidyesvpvnLlkd.GeqkkeefkalNPqelvPtLkide.gevltqSlAiieyLeety 77 l +y+rSsa+yRvRiaL+Lkg+d e ++v+L+k+ Geq+ +e++alNPqelvPtL+ ++ + vl+qSlAiieyL+e+y lcl|FitnessBrowser__PV4:5208938 3 LLGYWRSSAAYRVRIALNLKGLDAELESVHLVKNgGEQHLPEYAALNPQELVPTLIEGDnEFVLSQSLAIIEYLDEQY 80 789*******************************9********************99773679*************** PP TIGR01262 78 pepaLlpkdpakrarvralalliacdihPlqNlrvlqlleeklgvdeeekkewlkhwiekGlaalEellkekagafcv 155 +lp+ p++ra+vra+al iac++hPl+Nl+vlq+l+++l+ de++k++w++hwi++G++a+E+ l+++ag++c lcl|FitnessBrowser__PV4:5208938 81 GGAMMLPTAPKARAEVRAMALSIACEVHPLNNLKVLQYLTNTLELDEDAKNAWYHHWIHQGFSAFEKQLEKHAGSYCY 158 ****************************************************************************** PP TIGR01262 156 GdevtladvcLvpqvynAerfevdlaqyPtlkrieealaelpafqeahpenqpdt 210 Gdevtlad+cL+pqvynA+rf+vdl++yP ++ri +++++l+af a+pe+q d+ lcl|FitnessBrowser__PV4:5208938 159 GDEVTLADLCLIPQVYNANRFKVDLSTYPNIRRIWDNCHQLEAFVLAAPERQADA 213 ****************************************************996 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (211 nodes) Target sequences: 1 (214 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.00 # Mc/sec: 8.56 // [ok]
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory