Align Osmoregulated proline transporter OpuE; Osmoprotectant uptake; Proline uptake system (characterized)
to candidate 5210585 Shew_3013 sodium/proline symporter (RefSeq)
Query= SwissProt::O06493 (492 letters) >FitnessBrowser__PV4:5210585 Length = 498 Score = 532 bits (1370), Expect = e-155 Identities = 265/481 (55%), Positives = 365/481 (75%), Gaps = 6/481 (1%) Query: 7 ISLGIYFIAMLLIGWYAFKKTTD-INDYMLGGRGLGPFVTALSAGAADMSGWMLMGVPGA 65 ISL IYF+AML IG +A++++TD ++ Y+LGGR + P VTALSAGA+DMSGWMLMG+PGA Sbjct: 7 ISLAIYFLAMLAIGLFAYRRSTDDVSGYILGGRQVSPQVTALSAGASDMSGWMLMGLPGA 66 Query: 66 MFATGLSTLWLALGLTIGAYSNYLLLAPRLRAYTEAADDAITIPDFFDKRFQHSSSLLKI 125 MF G T+++ALGL +GA NYL++AP+LR YTE A++A+TIP+FF KRF S ++I Sbjct: 67 MFLVGFETIYIALGLLLGALVNYLIVAPKLRVYTEVANNALTIPEFFAKRFHDPSCNVRI 126 Query: 126 VSALIIMIFFTLYTSSGMVSGGRLFESAFGADYKLGLFLTTAVVVLYTLFGGFLAVSLTD 185 +SA II+IFFTLYTS+G+V+GG+LFESAF +Y+LGL +T VVV YTL GGFLAVSLTD Sbjct: 127 ISAAIIVIFFTLYTSAGLVAGGKLFESAFEVNYELGLVITLGVVVSYTLLGGFLAVSLTD 186 Query: 186 FVQGAIMFAALVLVPIVAFTHVGGVAPTFHEIDAVNPHLLDIFKGASVISIISYLAWGLG 245 FVQG IMF AL+LVP+VA+ + PH + + +++ +IS L+WGLG Sbjct: 187 FVQGCIMFVALILVPVVAYQEFSNADKMMNFAYESIPHFGEAMQNVTLLGLISALSWGLG 246 Query: 246 YYGQPHIIVRFMAIKDIKDLKPARRIGMSWMIITVLGSVLTGLIGVAYAHKFGVAVKDPE 305 Y+GQPHIIVRFMAI+ + D+K A+ IGMSWM +T++G++ TGL+G+AYA+KF + + DPE Sbjct: 247 YFGQPHIIVRFMAIRSVSDIKTAKNIGMSWMTVTIIGALATGLVGIAYANKFDMKLSDPE 306 Query: 306 MIFIIFSKILFHPLITGFLLSAILAAIMSSISSQLLVTASAVTEDLYRSFFRRKASDKEL 365 IFI+FS++LFHPLI+GFLL+AILAAIMS+ISSQLLV++S++TED+YR +++AS++E+ Sbjct: 307 TIFIVFSELLFHPLISGFLLAAILAAIMSTISSQLLVSSSSLTEDIYRVVSKKEASEEEM 366 Query: 366 VMIGRLSVLVIAVIAVLLSLNPNSTILDLVGYAWAGFGSAFGPAILLSLYWKRMNEWGAL 425 V +GR V +A++A LL+L+ +++IL LV AWAGFG+AFGP +L SLY K + A+ Sbjct: 367 VKLGRFGVAGVAIVASLLALDRSNSILSLVSNAWAGFGAAFGPLVLFSLYKKNLTHKAAV 426 Query: 426 AAMIVGAATVLIWI-----TTGLAKSTGVYEIIPGFILSMIAGIIVSMITKRPAKASYRL 480 A ++ GAATVL WI G A S+ +YE+IPGF+ S + VS + P + + Sbjct: 427 AGIVAGAATVLFWIYAPVLPEGKALSSMLYEMIPGFMASSLVIWGVSALDGDPCENTIET 486 Query: 481 F 481 F Sbjct: 487 F 487 Lambda K H 0.328 0.142 0.421 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 752 Number of extensions: 42 Number of successful extensions: 4 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 492 Length of database: 498 Length adjustment: 34 Effective length of query: 458 Effective length of database: 464 Effective search space: 212512 Effective search space used: 212512 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 52 (24.6 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory