Alignments for a candidate for lctP in Shewanella loihica PV-4

GapMind for catabolism of small carbon sources

Alignments for a candidate for lctP in Shewanella loihica PV-4

Align L-lactate permease (characterized, see rationale)
to candidate 5210707 Shew_3135 L-lactate permease (RefSeq)

Query= uniprot:A0KZB2
         (547 letters)



>FitnessBrowser__PV4:5210707
          Length = 547

 Score =  959 bits (2478), Expect = 0.0
 Identities = 483/546 (88%), Positives = 516/546 (94%)

Query: 1   MTILQLLASLTPVISVMIFLVLLRMPASKAMPISMIITALAAVFIWQMDTTLLAASVLEG 60
           MT+LQLLASLTPVISVM+FLVLL+MPAS+AMPISM++T LAAVFIWQMDTT+L ASV+EG
Sbjct: 1   MTLLQLLASLTPVISVMLFLVLLKMPASRAMPISMVMTGLAAVFIWQMDTTILGASVVEG 60

Query: 61  LLSAITPLTIIFGAVFLLNTLKYSGAMDTIRAGFTNISADARVQVIIICWLFGAFIEGSA 120
           LLSA+TPL+IIFGAVFLLNTLKYSGAMDTIRAGFTNIS DARVQVIIICWLFG+FIEGSA
Sbjct: 61  LLSALTPLSIIFGAVFLLNTLKYSGAMDTIRAGFTNISGDARVQVIIICWLFGSFIEGSA 120

Query: 121 GFGTPAAIGAPLLVLLGVPPVAAAVVALIADSACVSFGAIGLPVLFGMEQGLTQGGVSLA 180
           GFGTPAAIGAPLLVLLGVPPVAAAVVALIADS  VSFGAIGLPVLFGMEQGL QGGVS+A
Sbjct: 121 GFGTPAAIGAPLLVLLGVPPVAAAVVALIADSTSVSFGAIGLPVLFGMEQGLMQGGVSMA 180

Query: 181 AEQFAAHGGSYVGYARFIVMHMITIDLITGTLIPLVMVTILTGFFGRNKSFKEGLAIWKF 240
           A+Q A HG S+  YA+FI +HMITIDL+TGTLIPLV+V++LTGFFGRNKSFKEGLAIWKF
Sbjct: 181 ADQIAQHGTSFADYAQFIALHMITIDLVTGTLIPLVLVSVLTGFFGRNKSFKEGLAIWKF 240

Query: 241 AIFAGLAFTVPAWIINYLAGPEFPSVIGSLVGMALVIPVARKGYLLPKTPWNDFAENDSQ 300
           A+FAGLAFTVPAW+INY AGPEFPSVIGSLVGMALVIPVARKG LLPKTPWNDFAEND+Q
Sbjct: 241 ALFAGLAFTVPAWLINYFAGPEFPSVIGSLVGMALVIPVARKGLLLPKTPWNDFAENDNQ 300

Query: 301 EGAKIETTAKFSQIAAWTPYIIMAALLVLSRTVAPLKAWLSSFNISWTGLMGTELKASFA 360
             AK+ET A+FSQ+AAWTPYIIMA LLVLSRTVAPLKAWL+SFNISWT L+GTELKA FA
Sbjct: 301 TQAKVETKAQFSQVAAWTPYIIMAGLLVLSRTVAPLKAWLTSFNISWTNLLGTELKAGFA 360

Query: 361 TLYAPGAFFVAVCILGFFLFKMKSPAIKQSIGVSCKSMLPTIISLGASVPMVKIFLNSGV 420
           TLYAPGAFFV VCILGF LFKMKSPAIK+SI VSCKSMLPTIISLGASVPMVKIFLNSG 
Sbjct: 361 TLYAPGAFFVLVCILGFVLFKMKSPAIKESITVSCKSMLPTIISLGASVPMVKIFLNSGT 420

Query: 421 NGAGLASMPVALADMLGQSMGAVWAWMAPIVGIFGAFLSGSATFSNMMFSSLQYSVADNI 480
           N AGL SMPVALADML  SMGA+WAWM+PIVGIFGAFLSGSATFSNMMFS LQYSVADNI
Sbjct: 421 NEAGLQSMPVALADMLAGSMGAIWAWMSPIVGIFGAFLSGSATFSNMMFSGLQYSVADNI 480

Query: 481 GMNHTLVLALQGIGANAGNMMCVMNVVAAATVVGMAGRESEIIRKTMPVAIGYALLAGTI 540
           GMNH LVLALQGIGANAGNMMCVMNVVAAATVVGMAGRESEIIRKTMPVA+GYAL+AGTI
Sbjct: 481 GMNHALVLALQGIGANAGNMMCVMNVVAAATVVGMAGRESEIIRKTMPVALGYALIAGTI 540

Query: 541 ATLWGG 546
           ATLWGG
Sbjct: 541 ATLWGG 546


Lambda     K      H
   0.326    0.138    0.413 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1150
Number of extensions: 42
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 547
Length of database: 547
Length adjustment: 36
Effective length of query: 511
Effective length of database: 511
Effective search space:   261121
Effective search space used:   261121
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources