Alignments for a candidate for puuB in Shewanella loihica PV-4

GapMind for catabolism of small carbon sources

Alignments for a candidate for puuB in Shewanella loihica PV-4

Align gamma-glutamylputrescine oxidase (EC 1.4.3.-) (characterized)
to candidate 5208457 Shew_0969 FAD dependent oxidoreductase (RefSeq)

Query= reanno::pseudo5_N2C3_1:AO356_21495
         (427 letters)



>FitnessBrowser__PV4:5208457
          Length = 434

 Score =  361 bits (927), Expect = e-104
 Identities = 174/421 (41%), Positives = 265/421 (62%), Gaps = 3/421 (0%)

Query: 6   YPESYYAASANPVPPRPALQDDVETDVCVIGAGYTGLSSALFLLENGFKVTVLEAAKVGF 65
           YP+S+Y A+AN +   P L++ +  DVC++G G++G+++A+ L E GF+V +LEA ++G+
Sbjct: 12  YPDSFYHATANALEALPQLKERISADVCIVGGGFSGINTAIELAERGFEVVLLEAKRIGW 71

Query: 66  GASGRNGGQIVNSYSRDIDVIERSVGPQQAQLLGNMAFEGGRIIRERVAKYQIQCDLKDG 125
           GASGRNGG+++     +++     +G +  + +  M FE   I+R RVAK+ I C+L+ G
Sbjct: 72  GASGRNGGELIRGIGHNVEQFRNEIGSEGVEAIERMGFEAVEIVRNRVAKHNIDCNLQMG 131

Query: 126 GVFAALTAKQMGHLESQKRLWERFGHTQLELLDQRRIREVVACEEYVGGMLDMSGGHIHP 185
               A   + M  L +  +     G    +LL++ ++ EV+  + Y G ++DM+ GH+HP
Sbjct: 132 YCDLATKPRHMKELAADYQEMLHQGREGFKLLEKHQLGEVIGSDLYCGALVDMNSGHLHP 191

Query: 186 LNLALGEAAAVESLGGVIYEQSPAVRIERGASPVVHTPQGKVRAKFIIVAGNAYLGN-LV 244
           LNLALGEA    SLG  ++E S A +I +G  P VHT  G+V  +++++AGNAY+G+ L 
Sbjct: 192 LNLALGEAKVARSLGVKMFEYSAATKIVKGERPCVHTEHGRVDCRYLVLAGNAYIGHELN 251

Query: 245 PELAAKSMPCGTQVIATEPLGDELAHSLLPQDYCVEDCNYLLDYYRLTGDKRLIFGGGVV 304
             +  K +P G+ ++ATEPL  E+   ++PQ+    D    LDY+ L+ D RL+FGG   
Sbjct: 252 GYIGGKVLPAGSYLLATEPLSQEMCDEIIPQNMAFADMRIDLDYFHLSQDNRLLFGGLCT 311

Query: 305 YGARDPANIEAIIRPKMLKAFPQLKDVKIDYAWTGNFLLTLSRLPQVGRLGD--NIYYSQ 362
           Y  +DP +I+A +RP + K FPQLK VKI+Y W G   +  +RLPQ+GRL D  NI Y+Q
Sbjct: 312 YSGKDPKDIDAALRPNLEKVFPQLKGVKIEYEWGGMIGIGANRLPQIGRLPDCPNILYAQ 371

Query: 363 GCSGHGVTYTHLAGKVLAEALRGQAERFDAFADLPHYPFPGGQLLRTPFAAMGAWYYGLR 422
             +GHGV  TH+A +++ EAL  QAERFD F  + H  FPGG   R+P  A G  Y+ L+
Sbjct: 372 AYAGHGVNATHMAARLIGEALTAQAERFDIFDRVQHMTFPGGPRFRSPMLAAGMLYHRLK 431

Query: 423 D 423
           D
Sbjct: 432 D 432


Lambda     K      H
   0.320    0.139    0.421 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 593
Number of extensions: 30
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 427
Length of database: 434
Length adjustment: 32
Effective length of query: 395
Effective length of database: 402
Effective search space:   158790
Effective search space used:   158790
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources