GapMind for catabolism of small carbon sources

 

Aligments for a candidate for aldA in Shewanella loihica PV-4

Align NAD(P)+ L-lactaldehyde dehydrogenase (EC 1.2.1.22) (characterized)
to candidate 5211158 Shew_3574 aldehyde dehydrogenase (RefSeq)

Query= metacyc::MONOMER-16244
         (495 letters)



>lcl|FitnessBrowser__PV4:5211158 Shew_3574 aldehyde dehydrogenase
           (RefSeq)
          Length = 506

 Score =  336 bits (862), Expect = 1e-96
 Identities = 193/475 (40%), Positives = 281/475 (59%), Gaps = 15/475 (3%)

Query: 24  FINNEFVQSKSKKTFGTVSPSTEEEITQVYEAFSEDIDDAVEAATAAFHSSWSTSDPQVR 83
           FI  ++V     + F   SP   +   QV  +   DI+ A++AA AA   +W  +    R
Sbjct: 22  FIGGQWVAPVGGEYFDNRSPVDGQVFCQVARSDERDIELALDAAHAA-KDAWGKTSVTER 80

Query: 84  MKVLYKLADLIDEHADTLAHIEALDNGKSLMCS-KGDVALTAAYFRSCAGWTDKIKGSVI 142
             +L K+AD ++++ + LA  E  +NGK++  +   D+ L   +FR  AG     +GS  
Sbjct: 81  SNLLLKIADRVEQNLEFLAVAETWENGKAVRETLNADLPLFVDHFRYFAGCIRAQEGSAA 140

Query: 143 ETGDTHFNYTRREPIGVCGQIIPWNFPLLMASWKLGPVLCTGCTTVLKTAESTPLSALYL 202
           +  +   +Y   EP+GV GQIIPWNFPLLMA+WK+ P L  G   VLK AE TP+S L +
Sbjct: 141 DIDNNTVSYHFPEPLGVVGQIIPWNFPLLMAAWKIAPALAAGNCIVLKPAEQTPVSILVM 200

Query: 203 ASLIKEAGAPPGVVNVVSGFGPTAGAPISSHPKIKKVAFTGSTATGRHIMKAAAESNLKK 262
             LI++   P GV+N+V+GFG  AG  ++   +I K+AFTGST  G HI+K AAES +  
Sbjct: 201 VELIQDL-LPAGVLNIVNGFGTEAGQALAVSKRIAKLAFTGSTQIGHHILKCAAESLIPS 259

Query: 263 VTLELGGKSPNIVFDDADVKST--IQHLVTGI---FYNTGEVCCAGSRIYVQEGIYDKIV 317
            T+ELGGKSPNI F D   +    +   V G+   F+N GEVC   SR+ + E IYDK +
Sbjct: 260 -TVELGGKSPNIYFADVMEQEDEYLDKAVEGMLLAFFNQGEVCTCPSRVLIAESIYDKFI 318

Query: 318 SEFKNAAESLKIGDPFKEDTFMGAQTSQLQLDKILKYIDIGKKEGATVITGGERFG---- 373
            +    A+++K G+P   DT +GAQ SQ Q DKIL Y++IG+ EGA V+ GG        
Sbjct: 319 DKVIARAKTIKQGNPLDTDTQVGAQASQEQFDKILSYLEIGRNEGAEVLIGGTSCQLSGD 378

Query: 374 -NKGYFIKPTIFGDVKEDHQIVRDEIFGPVVTITKFKTVEEVIALANDSEYGLAAGVHTT 432
            + G++I+PTI     +  ++ ++EIFGPV+++T FK   E +A+AND+EYGL AGV T 
Sbjct: 379 QSSGFYIEPTILKGHNK-MRVFQEEIFGPVISVTTFKDEAEALAIANDTEYGLGAGVWTR 437

Query: 433 NLSTAISVSNKINSGTIWVNTYNDFHPMVPFGGYSQSGIGREMGEEALDNYTQVK 487
           +++ A  +   I +G +W+N Y+ +     FGGY +SGIGRE  +  L +Y   K
Sbjct: 438 DMNRAQRMGRGIQAGRVWINCYHAYPAHAAFGGYKKSGIGRETHKMMLSHYQNTK 492


Lambda     K      H
   0.316    0.133    0.389 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 617
Number of extensions: 31
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 495
Length of database: 506
Length adjustment: 34
Effective length of query: 461
Effective length of database: 472
Effective search space:   217592
Effective search space used:   217592
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory