Align thymidine phosphorylase (EC 2.4.2.4) (characterized)
to candidate 5210371 Shew_2814 thymidine phosphorylase (RefSeq)
Query= BRENDA::P07650 (440 letters) >FitnessBrowser__PV4:5210371 Length = 443 Score = 611 bits (1576), Expect = e-179 Identities = 298/440 (67%), Positives = 358/440 (81%) Query: 1 MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAMTIFFHDMTMPERVSLT 60 MFLAQEIIRKKR+G LS +EI+FF+ GI +NT+SEGQIAAL M ++F DM M ER++LT Sbjct: 1 MFLAQEIIRKKRNGEVLSTQEIQFFVQGITNNTVSEGQIAALGMAVYFKDMNMDERIALT 60 Query: 61 MAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMVAACGGYIPMISGRGLGHT 120 AMRDSGTVL+WK+L L+GPI+DKHSTGGVGDV SLMLGPM AACGGY+PMISGRGLGHT Sbjct: 61 TAMRDSGTVLNWKNLGLDGPIIDKHSTGGVGDVISLMLGPMAAACGGYVPMISGRGLGHT 120 Query: 121 GGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSLAPADKRFYATRDITATVDSI 180 GGTLDK ++IPG+ PD + FR+++K+ GVAIIGQT L PADKRFY+ RD TATV+SI Sbjct: 121 GGTLDKFDAIPGYQTEPDSDLFRKVVKEAGVAIIGQTGDLVPADKRFYSIRDNTATVESI 180 Query: 181 PLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSEALAEAIVGVANGAGVRTTALLT 240 LITASIL+KKLA GLDAL MDVKVGSGAFMPTYE SE LA +I VANGAG +TTALLT Sbjct: 181 SLITASILSKKLAAGLDALAMDVKVGSGAFMPTYEASEELARSITAVANGAGTKTTALLT 240 Query: 241 DMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVTMALCVEMLISGKLAKDDAEARAKL 300 DMNQVLAS AGNAVEV+EAV FLTG YRNPRL++VTM LC EML G LA +A+AR KL Sbjct: 241 DMNQVLASCAGNAVEVKEAVDFLTGAYRNPRLYEVTMGLCAEMLQLGGLAASEADAREKL 300 Query: 301 QAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYLPTAMLTKAVYADTEGFVSEMDTRALG 360 VLDNGKAA++FGRM+A GP DF+ENYAKYLP + + + VYAD GF + MDTR LG Sbjct: 301 NRVLDNGKAADIFGRMIAGLGGPADFIENYAKYLPQSQIIRPVYADRSGFAASMDTRELG 360 Query: 361 MAVVAMGGGRRQASDTIDYSVGFTDMARLGDQVDGQRPLAVIHAKDENNWQEAAKAVKAA 420 +AVV +GGGRR+ D +DYSVG T + LGD++ +P+A++HA+ E+ ++EAA AVK A Sbjct: 361 LAVVTLGGGRRKPGDALDYSVGLTQVCALGDEITSDKPIAMVHAQSESAFEEAAAAVKKA 420 Query: 421 IKLADKAPESTPTVYRRISE 440 I + D+APE TP +YR I + Sbjct: 421 IHIGDEAPEKTPEIYRYIRQ 440 Lambda K H 0.318 0.134 0.377 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 553 Number of extensions: 11 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 440 Length of database: 443 Length adjustment: 32 Effective length of query: 408 Effective length of database: 411 Effective search space: 167688 Effective search space used: 167688 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 51 (24.3 bits)
Align candidate 5210371 Shew_2814 (thymidine phosphorylase (RefSeq))
to HMM TIGR02643 (deoA: thymidine phosphorylase (EC 2.4.2.4))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.carbon/TIGR02643.hmm # target sequence database: /tmp/gapView.32396.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR02643 [M=437] Accession: TIGR02643 Description: T_phosphoryl: thymidine phosphorylase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 4.5e-226 736.7 4.9 5.1e-226 736.5 4.9 1.0 1 lcl|FitnessBrowser__PV4:5210371 Shew_2814 thymidine phosphorylas Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__PV4:5210371 Shew_2814 thymidine phosphorylase (RefSeq) # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 736.5 4.9 5.1e-226 5.1e-226 1 437 [] 2 438 .. 2 438 .. 1.00 Alignments for each domain: == domain 1 score: 736.5 bits; conditional E-value: 5.1e-226 TIGR02643 1 llpqeiirkkrdglslsdeeiaafvegvtddsvsegqiaalamavyfngleldekvaltlamrdsGdvldwkdldlng 78 +l+qeiirkkr+g+ ls++ei+ fv+g+t+++vsegqiaal mavyf+++++de++alt amrdsG+vl+wk+l l+g lcl|FitnessBrowser__PV4:5210371 2 FLAQEIIRKKRNGEVLSTQEIQFFVQGITNNTVSEGQIAALGMAVYFKDMNMDERIALTTAMRDSGTVLNWKNLGLDG 79 79**************************************************************************** PP TIGR02643 79 PvvdkhstGGvGdvvslmlaPivaacGgyvPmisGrGlGhtGGtldkleaiPGydvaPdeelfrkvvkdvGvaiiGqt 156 P++dkhstGGvGdv+slml+P+ aacGgyvPmisGrGlGhtGGtldk++aiPGy++ Pd++lfrkvvk++GvaiiGqt lcl|FitnessBrowser__PV4:5210371 80 PIIDKHSTGGVGDVISLMLGPMAAACGGYVPMISGRGLGHTGGTLDKFDAIPGYQTEPDSDLFRKVVKEAGVAIIGQT 157 ****************************************************************************** PP TIGR02643 157 adlaPadkrlyairdvtatvesiplitasilskklaaGldalvldvkvGnGafmesleeseelarslvevangaGvrt 234 +dl Padkr+y+ird tatvesi+litasilskklaaGldal++dvkvG+Gafm+++e seelars+ vangaG +t lcl|FitnessBrowser__PV4:5210371 158 GDLVPADKRFYSIRDNTATVESISLITASILSKKLAAGLDALAMDVKVGSGAFMPTYEASEELARSITAVANGAGTKT 235 ****************************************************************************** PP TIGR02643 235 talitdmnqalasaaGnavevreavdfltGekrdkrleevtlalaaellvsgklaadeaeaeakleavlesGraaerf 312 tal+tdmnq+las+aGnavev+eavdfltG++r++rl evt+ l+ae+l+ g+laa+ea+a+ kl++vl++G+aa++f lcl|FitnessBrowser__PV4:5210371 236 TALLTDMNQVLASCAGNAVEVKEAVDFLTGAYRNPRLYEVTMGLCAEMLQLGGLAASEADAREKLNRVLDNGKAADIF 313 ****************************************************************************** PP TIGR02643 313 armvaalgGPadfvekpekylakaaiakavkaaregylseidtrelGlavvalGGGrrkaddkldlsvGltdllelGe 390 +rm+a lgGPadf+e+++kyl++++i+++v+a+r+g+ +++dtrelGlavv+lGGGrrk+ d ld+svGlt++ lG+ lcl|FitnessBrowser__PV4:5210371 314 GRMIAGLGGPADFIENYAKYLPQSQIIRPVYADRSGFAASMDTRELGLAVVTLGGGRRKPGDALDYSVGLTQVCALGD 391 ****************************************************************************** PP TIGR02643 391 kvekeeplavvhaadeedaeeaakavkkalkiadeaPeeakvvleri 437 ++ +++p+a+vha++e+ +eeaa+avkka++i+deaPe+++ +++ i lcl|FitnessBrowser__PV4:5210371 392 EITSDKPIAMVHAQSESAFEEAAAAVKKAIHIGDEAPEKTPEIYRYI 438 *******************************************9876 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (437 nodes) Target sequences: 1 (443 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.02u 0.01s 00:00:00.03 Elapsed: 00:00:00.02 # Mc/sec: 6.66 // [ok]
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory