Alignments for a candidate for treF in Shewanella loihica PV-4

GapMind for catabolism of small carbon sources

Alignments for a candidate for treF in Shewanella loihica PV-4

Align Alpha-glucosidase; EC 3.2.1.- (characterized, see rationale)
to candidate 5209418 Shew_1889 alpha amylase, catalytic region (RefSeq)

Query= uniprot:A8LLL3
         (552 letters)



>FitnessBrowser__PV4:5209418
          Length = 541

 Score =  596 bits (1536), Expect = e-175
 Identities = 277/500 (55%), Positives = 351/500 (70%), Gaps = 5/500 (1%)

Query: 19  WWRGAVIYQIYPRSFQDSNGDGIGDLLGIVERMPYIASLGVDAIWISPFFTSPMKDFGYD 78
           WWRGAVIYQIYPRS  DSNGDG+GDL GI+ ++ YIASL VDAIWISPFF SPMKDFGYD
Sbjct: 6   WWRGAVIYQIYPRSLMDSNGDGVGDLQGIISKLDYIASLNVDAIWISPFFKSPMKDFGYD 65

Query: 79  ISDYFDVDPMFGSLADFDALIETAHMYGLRVMIDLVLSHTSDQHPWFEESRSSRDNPKAD 138
           ISDY  +DP+FG++ADFD LI+ AH  G++V+ID VLSHTSDQH WF ESR SRDN K D
Sbjct: 66  ISDYRAIDPLFGTMADFDELIDKAHGLGIKVIIDQVLSHTSDQHAWFSESRQSRDNAKQD 125

Query: 139 WYVWADAKPDGTPPNNWLSIFGGSGWHWDARRCQYYLHNFLTSQPDLNFHCADVQDALLG 198
           WYVWAD  PDGT PNNWL+IFGG  W W+ RR QYYLHNFLTSQPDLNFH  DV+ A+L 
Sbjct: 126 WYVWADPNPDGTAPNNWLAIFGGCAWEWEPRRQQYYLHNFLTSQPDLNFHNPDVRQAVLD 185

Query: 199 VGRFWLDRGVDGFRLDTINFYVHDAELRDNPPLPPEERNSNIAPEVNPYNHQRHLYSKNQ 258
             +FWLD+GVDGFRLD I F  HD +LRDNPP P ++R      E NPY +Q H Y+  +
Sbjct: 186 NVKFWLDKGVDGFRLDAITFCYHDEQLRDNPPKPEDKRQGRGFSEDNPYAYQYHYYNNTR 245

Query: 259 PENLEFLAKFRAMMEEYPAIAAVGEVGDAQYGLEILGQYTRGETGVHMCYAFEFLAQEKL 318
           P+ + F+ + RA++ +YP +  +GEV  ++  L  + +YT+G+  +HM Y+FE L  +  
Sbjct: 246 PQTVGFIEELRALINQYPGVVTLGEV-SSEDSLATMAEYTQGDDRLHMAYSFELLTDD-F 303

Query: 319 TAKRVAEVLNKVDEVASDGWACWAFSNHDVMRHVSRW--DLTPGAQRGMLTLLMC-LRGS 375
           +   +   +  +++   DGW CWA  NHDV R  +RW  D        ML  ++C LRGS
Sbjct: 304 SPAYIRHTVEALEQSIGDGWPCWAIGNHDVQRVATRWGRDAFNSDMAKMLNAMLCSLRGS 363

Query: 376 VCLYQGEELGLPEAEVAFDDLQDPYGIEFWPEYKGRDGCRTPMVWQSDNMSGGFSIHRPW 435
           VC YQGEELGL E E+A++ LQDP+GI FWP +KGRDGCRTPM W+ D    GFS + PW
Sbjct: 364 VCSYQGEELGLGEVEIAYEQLQDPFGITFWPMFKGRDGCRTPMPWRHDANHAGFSDNTPW 423

Query: 436 LPVSTEHLGLAVAVQEEAPDALLHHYRRALAFRRAHPALVKGDISDVTVVGDVISFLRKD 495
           LPV  +H  +AV VQE  P+++L+HYR  LA+R+ HP LV GDI  +     ++ F R+ 
Sbjct: 424 LPVPADHKAVAVDVQEANPESILNHYRHMLAWRKLHPILVTGDIKFIESTDALLVFERRL 483

Query: 496 PEETVFVAINMSDAPGAVDL 515
            E+T+ VA N+S  P +  L
Sbjct: 484 GEQTLLVAFNLSGQPQSFSL 503


Lambda     K      H
   0.321    0.138    0.451 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1007
Number of extensions: 36
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 552
Length of database: 541
Length adjustment: 36
Effective length of query: 516
Effective length of database: 505
Effective search space:   260580
Effective search space used:   260580
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources