Align Homogentisate 1,2-dioxygenase (EC 1.13.11.5) (characterized)
to candidate 5209701 Shew_2154 homogentisate 1,2-dioxygenase (RefSeq)
Query= reanno::MR1:201124 (386 letters) >FitnessBrowser__PV4:5209701 Length = 386 Score = 729 bits (1882), Expect = 0.0 Identities = 330/386 (85%), Positives = 366/386 (94%) Query: 1 MPFYVKQGQVPHKRHIAFEKENGELYREELFSTHGFSNIYSNKYHHNMPTKALEVAPYRL 60 MPFYV+QG++PHKRHI F+KENGELYREELFSTHGFSNIYSNKYHHNMPTKALEV+P+ + Sbjct: 1 MPFYVRQGEIPHKRHITFKKENGELYREELFSTHGFSNIYSNKYHHNMPTKALEVSPFEV 60 Query: 61 GHGAHWEDSLVQNYKLDSRSADREGNFFSARNKIFYNNDVAIYTAKVTQDTAEFYRNAYA 120 HG W+D+L+QNYKLD++ ADREGNF+SARNKIF+NNDVA+Y+AKVT+ T EFYRNAYA Sbjct: 61 NHGQTWQDTLIQNYKLDAKLADREGNFYSARNKIFFNNDVAMYSAKVTEATEEFYRNAYA 120 Query: 121 DEVVFVHEGEGTLYSEYGTLEIKKWDYLVIPRGTTHQLKFNNYSNVRLFVIEAFSMVEVP 180 DEV+FVHEG+G LYSEYG L++KKWDYLVIPRGTT+QLKF++YS VRLFVIEAFSMVEVP Sbjct: 121 DEVIFVHEGQGKLYSEYGVLDVKKWDYLVIPRGTTYQLKFDDYSQVRLFVIEAFSMVEVP 180 Query: 181 KHCRNEYGQLLESAPYCERDLRTPILQAAVVERGAFPLVCKFGDKYQLTTLEWHPFDLVG 240 KH RNEYGQLLESAPYCERD+R P LQ AVVE+GAFPLVCKFGDKYQLT LEWHPFDLVG Sbjct: 181 KHFRNEYGQLLESAPYCERDIRVPSLQEAVVEKGAFPLVCKFGDKYQLTQLEWHPFDLVG 240 Query: 241 WDGCVYPWAFNITEYAPKVGKIHLPPSDHLVFTAHNFVVCNFVPRPYDFHERAIPAPYYH 300 WDGCVYPWAFNI +YAPKVG+IHLPPSDHLVFTAHNFV+CNFVPRPYDFH ++IPAPYYH Sbjct: 241 WDGCVYPWAFNIQDYAPKVGQIHLPPSDHLVFTAHNFVICNFVPRPYDFHPQSIPAPYYH 300 Query: 301 NNIDSDEVLYYVDGDFMSRTGIEAGYITLHQKGVAHGPQPGRTEASIGKKETYEYAVMVD 360 NNIDSDEVLYYVDGDFMSRTGIEAGY+TLHQKGV HGPQPGRTEAS+GKKETYEYAVMVD Sbjct: 301 NNIDSDEVLYYVDGDFMSRTGIEAGYMTLHQKGVPHGPQPGRTEASVGKKETYEYAVMVD 360 Query: 361 TFAPLKLTEHVQHCMSKDYNRSWLEN 386 TFAPL+LT+HVQ CMSKDYNRSWLE+ Sbjct: 361 TFAPLQLTQHVQGCMSKDYNRSWLED 386 Lambda K H 0.321 0.137 0.435 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 749 Number of extensions: 26 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 386 Length of database: 386 Length adjustment: 30 Effective length of query: 356 Effective length of database: 356 Effective search space: 126736 Effective search space used: 126736 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 50 (23.9 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory