GapMind for catabolism of small carbon sources

 

Protein CA265_RS19000 in Pedobacter sp. GW460-11-11-14-LB5

Annotation: CA265_RS19000 NADP-dependent malic enzyme

Length: 771 amino acids

Source: Pedo557 in FitnessBrowser

Candidate for 9 steps in catabolism of small carbon sources

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
4-hydroxybenzoate catabolism pta lo Phosphate acetyltransferase; EC 2.3.1.8; Phosphotransacetylase (uncharacterized) 30% 91% 217.2 malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+) (EC 1.1.1.40) 50% 699.1
L-lactate catabolism pta lo Phosphate acetyltransferase; EC 2.3.1.8; Phosphotransacetylase (uncharacterized) 30% 91% 217.2 malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+) (EC 1.1.1.40) 50% 699.1
acetate catabolism pta lo Phosphate acetyltransferase; EC 2.3.1.8; Phosphotransacetylase (uncharacterized) 30% 91% 217.2 malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+) (EC 1.1.1.40) 50% 699.1
2'-deoxyinosine catabolism pta lo Phosphate acetyltransferase; EC 2.3.1.8; Phosphotransacetylase (uncharacterized) 30% 91% 217.2 malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+) (EC 1.1.1.40) 50% 699.1
2-deoxy-D-ribose catabolism pta lo Phosphate acetyltransferase; EC 2.3.1.8; Phosphotransacetylase (uncharacterized) 30% 91% 217.2 malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+) (EC 1.1.1.40) 50% 699.1
ethanol catabolism pta lo Phosphate acetyltransferase; EC 2.3.1.8; Phosphotransacetylase (uncharacterized) 30% 91% 217.2 malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+) (EC 1.1.1.40) 50% 699.1
L-threonine catabolism pta lo Phosphate acetyltransferase; EC 2.3.1.8; Phosphotransacetylase (uncharacterized) 30% 91% 217.2 malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+) (EC 1.1.1.40) 50% 699.1
thymidine catabolism pta lo Phosphate acetyltransferase; EC 2.3.1.8; Phosphotransacetylase (uncharacterized) 30% 91% 217.2 malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+) (EC 1.1.1.40) 50% 699.1
L-tryptophan catabolism pta lo Phosphate acetyltransferase; EC 2.3.1.8; Phosphotransacetylase (uncharacterized) 30% 91% 217.2 malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+) (EC 1.1.1.40) 50% 699.1

Sequence Analysis Tools

View CA265_RS19000 at FitnessBrowser

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search PFam (including for weak hits, up to E = 1)

Predict protein localization: PSORTb (Gram negative bacteria)

Predict transmembrane helices: TMHMM

Check the SEED with FIGfam search

Fitness BLAST: loading...

Sequence

MSKTTRKQDALDYHSQGRPGKIQVVPTKPTTSQRDLTLAYSPGVAEPCLKIAENKEDVYK
YTAKGNLVAVISNGTAVLGLGDIGPEAGKPVMEGKGLLFKIFADIDVFDLELDTKNVDDF
VKIVKALEPTFGGVNLEDIKAPECFEIERRLKEEMNIPVMHDDQHGTAIISAAALLNACE
IFKKKMDKIKIVVNGAGAAAISCTKLYVSLGAKKENIIMCDRSGVIRKDREVLDEIKAEF
ATDRKISTLAEAMKDSDVFIGLSSADCVTAEMLTSMAKNPIVFAMANPNPEIAYELAIKT
RKDIIMATGRSDYPNQVNNVLGFPYIFRGALDVRATGINEPMKIAAVKAIAELAKKSVPE
AVNLAYNARNLKFGKEYIIPKPVDFRLITEVSIAVAKAAIESGVARKIITDWDAYSEELR
KRLGLDDAIMRAITTKAKTDPKRVVFAEADNYKILKAAQIVNDENIAIPILLGNKDKIQA
IIDEHGLELEGVEIIDQMQNPDKTKQYAEALYQKRQRKGVSLTDATKLLRDRNYYGASMV
EFGEADAMISGLTKNYGSTIKPALHVIGVDPSVKRVAGMYMMMTKKGPVFFGDTTVNVDP
TAEELVDITLLLDKSVKQFNIKPRIALLSYSNFGSNDGVTPNKVRETVKLLHKNHPEVIV
DGEMQGNFAINNELLKDNFPFSTLADAPANTLVFPNLESGNIAYKLLQELGGAEAVGPIL
LGLNKPVHIVQLGSSVREIVNMVTIAVVDVQAKEEIATSKRKGIFGKTTKK

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory