Align Glucosamine-6-phosphate deaminase (EC 3.5.99.6) (characterized)
to candidate CA265_RS21925 CA265_RS21925 glucosamine-6-phosphate deaminase
Query= reanno::Pedo557:CA265_RS21925 (641 letters) >FitnessBrowser__Pedo557:CA265_RS21925 Length = 641 Score = 1306 bits (3381), Expect = 0.0 Identities = 641/641 (100%), Positives = 641/641 (100%) Query: 1 MARLNLLEETRFEKLPVSVFENPKIASINVAHRIAELIKSKQANNTPAVLGLATGVTPIA 60 MARLNLLEETRFEKLPVSVFENPKIASINVAHRIAELIKSKQANNTPAVLGLATGVTPIA Sbjct: 1 MARLNLLEETRFEKLPVSVFENPKIASINVAHRIAELIKSKQANNTPAVLGLATGVTPIA 60 Query: 61 VYAELVRLHKEEGLSFKNVITFNLDEYYPMAPTAAQSYVTFMNENLFNHIDIDKKNVHIP 120 VYAELVRLHKEEGLSFKNVITFNLDEYYPMAPTAAQSYVTFMNENLFNHIDIDKKNVHIP Sbjct: 61 VYAELVRLHKEEGLSFKNVITFNLDEYYPMAPTAAQSYVTFMNENLFNHIDIDKKNVHIP 120 Query: 121 DGTLALEDIPAFCLEYEKKIGDLGGLDIQILGIGRTGHIGFNEPGSAPNSGTRLVTLDDL 180 DGTLALEDIPAFCLEYEKKIGDLGGLDIQILGIGRTGHIGFNEPGSAPNSGTRLVTLDDL Sbjct: 121 DGTLALEDIPAFCLEYEKKIGDLGGLDIQILGIGRTGHIGFNEPGSAPNSGTRLVTLDDL 180 Query: 181 TRRDAARDFGGKTFVPTKAITMGIGTIFKAREIILMAWSRKKASIIKKAVEGEISGDVPA 240 TRRDAARDFGGKTFVPTKAITMGIGTIFKAREIILMAWSRKKASIIKKAVEGEISGDVPA Sbjct: 181 TRRDAARDFGGKTFVPTKAITMGIGTIFKAREIILMAWSRKKASIIKKAVEGEISGDVPA 240 Query: 241 TFLQLSDHVEFILDAPAASELTRFYTPWLVKDCVWTDALIRKAVIWLANTLKKPILKLTE 300 TFLQLSDHVEFILDAPAASELTRFYTPWLVKDCVWTDALIRKAVIWLANTLKKPILKLTE Sbjct: 241 TFLQLSDHVEFILDAPAASELTRFYTPWLVKDCVWTDALIRKAVIWLANTLKKPILKLTE 300 Query: 301 DDYNNNGMAQLATEKGPVYNINIHIFNKLQHTITGWPGGKPNADDSQRPERAEPAKKRVI 360 DDYNNNGMAQLATEKGPVYNINIHIFNKLQHTITGWPGGKPNADDSQRPERAEPAKKRVI Sbjct: 301 DDYNNNGMAQLATEKGPVYNINIHIFNKLQHTITGWPGGKPNADDSQRPERAEPAKKRVI 360 Query: 361 IFSPHPDDDVISMGGTFLRLVDQGHDVHVAYQTSGNTAVWDDDALRFVEFNVDFTEKMGM 420 IFSPHPDDDVISMGGTFLRLVDQGHDVHVAYQTSGNTAVWDDDALRFVEFNVDFTEKMGM Sbjct: 361 IFSPHPDDDVISMGGTFLRLVDQGHDVHVAYQTSGNTAVWDDDALRFVEFNVDFTEKMGM 420 Query: 421 DNTHLKDLYNKMRAFIEQKKPNQIDTPEIQTVKGLIRKGEAIAGARYCGLEDDHIHFQAL 480 DNTHLKDLYNKMRAFIEQKKPNQIDTPEIQTVKGLIRKGEAIAGARYCGLEDDHIHFQAL Sbjct: 421 DNTHLKDLYNKMRAFIEQKKPNQIDTPEIQTVKGLIRKGEAIAGARYCGLEDDHIHFQAL 480 Query: 481 PFYESGKVQKNPVTDADIELTIELLQKVKPQQVYAAGDFEDPHGTHIVCFNIILEALKRL 540 PFYESGKVQKNPVTDADIELTIELLQKVKPQQVYAAGDFEDPHGTHIVCFNIILEALKRL Sbjct: 481 PFYESGKVQKNPVTDADIELTIELLQKVKPQQVYAAGDFEDPHGTHIVCFNIILEALKRL 540 Query: 541 RKTEAWAQDCWLWMYRGAWHEFETHEIEMAVPISPQELERKKYAIFKHQSQKDRAVFPGD 600 RKTEAWAQDCWLWMYRGAWHEFETHEIEMAVPISPQELERKKYAIFKHQSQKDRAVFPGD Sbjct: 541 RKTEAWAQDCWLWMYRGAWHEFETHEIEMAVPISPQELERKKYAIFKHQSQKDRAVFPGD 600 Query: 601 DSREFWQRAEDRNRDTAKAYDELGLAEYEAMEAFVRWKFED 641 DSREFWQRAEDRNRDTAKAYDELGLAEYEAMEAFVRWKFED Sbjct: 601 DSREFWQRAEDRNRDTAKAYDELGLAEYEAMEAFVRWKFED 641 Lambda K H 0.319 0.136 0.412 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1522 Number of extensions: 51 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 641 Length of database: 641 Length adjustment: 38 Effective length of query: 603 Effective length of database: 603 Effective search space: 363609 Effective search space used: 363609 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 54 (25.4 bits)
Align candidate CA265_RS21925 CA265_RS21925 (glucosamine-6-phosphate deaminase)
to HMM TIGR00502 (nagB: glucosamine-6-phosphate deaminase (EC 3.5.99.6))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.carbon/TIGR00502.hmm # target sequence database: /tmp/gapView.27626.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00502 [M=259] Accession: TIGR00502 Description: nagB: glucosamine-6-phosphate deaminase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 2.4e-77 245.8 0.0 7.6e-77 244.1 0.0 1.7 2 lcl|FitnessBrowser__Pedo557:CA265_RS21925 CA265_RS21925 glucosamine-6-phos Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__Pedo557:CA265_RS21925 CA265_RS21925 glucosamine-6-phosphate deaminase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 244.1 0.0 7.6e-77 7.6e-77 16 248 .. 30 262 .. 18 267 .. 0.95 2 ? -2.0 0.0 0.093 0.093 123 150 .. 368 395 .. 366 398 .. 0.86 Alignments for each domain: == domain 1 score: 244.1 bits; conditional E-value: 7.6e-77 TIGR00502 16 aariiaekinefkpdaerpfvlGlatGgtPvglykqlielykagkvsfkkvvtfnldeyvglseehPe 83 a+ iae i+ k+ ++ p vlGlatG tP+ +y +l++l+k + +sfk+v+tfnldey + + + lcl|FitnessBrowser__Pedo557:CA265_RS21925 30 VAHRIAELIKS-KQANNTPAVLGLATGVTPIAVYAELVRLHKEEGLSFKNVITFNLDEYYPMAPTAAQ 96 46778888888.888899************************************************** PP TIGR00502 84 syhsfmyenffqhidikpeninilnGn..addleaecrryeekikslGkidlfllGiGadGhiafneP 149 sy +fm+en+f+hidi ++n++i++G +d+ a c +ye+ki +lG++d+++lGiG+ Ghi+fneP lcl|FitnessBrowser__Pedo557:CA265_RS21925 97 SYVTFMNENLFNHIDIDKKNVHIPDGTlaLEDIPAFCLEYEKKIGDLGGLDIQILGIGRTGHIGFNEP 164 **************************732589************************************ PP TIGR00502 150 gsslesrtrvktltedtiiansrffegdvnkvPkkaltvGiktildskevlllvsGkekaeavkklve 217 gs +s tr++tl + t+ +r f+g + vP ka+t+Gi+ti++++e++l++ ++ka+ +kk+ve lcl|FitnessBrowser__Pedo557:CA265_RS21925 165 GSAPNSGTRLVTLDDLTRRDAARDFGG-KTFVPTKAITMGIGTIFKAREIILMAWSRKKASIIKKAVE 231 *************************95.689************************************* PP TIGR00502 218 gsvnedvtisalqlhkkvivvadeeaaqelk 248 g ++ dv+++ lql ++v + d aa+el lcl|FitnessBrowser__Pedo557:CA265_RS21925 232 GEISGDVPATFLQLSDHVEFILDAPAASELT 262 ***************************9985 PP == domain 2 score: -2.0 bits; conditional E-value: 0.093 TIGR00502 123 ekikslGkidlfllGiGadGhiafnePg 150 + + s+G++ l l G+d h+a+ g lcl|FitnessBrowser__Pedo557:CA265_RS21925 368 DDVISMGGTFLRLVDQGHDVHVAYQTSG 395 567899*****************98665 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (259 nodes) Target sequences: 1 (641 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.00 # Mc/sec: 19.08 // [ok]
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory