Alignments for a candidate for arcA in Pedobacter sp. GW460-11-11-14-LB5

GapMind for catabolism of small carbon sources

Alignments for a candidate for arcA in Pedobacter sp. GW460-11-11-14-LB5

Align Arginine deiminase; ADI; EC 3.5.3.6; Arginine dihydrolase; AD (uncharacterized)
to candidate CA265_RS10470 CA265_RS10470 amidinotransferase

Query= curated2:Q0TUR5
         (413 letters)



>FitnessBrowser__Pedo557:CA265_RS10470
          Length = 481

 Score =  142 bits (358), Expect = 2e-38
 Identities = 141/493 (28%), Positives = 216/493 (43%), Gaps = 107/493 (21%)

Query: 7   LNVTSEIGRLKTVLLHRPGEEIENLTPDLLDRLLFDDIPYLKVARE-EHDAFAQTLREAG 65
           ++V +EIGRL+ +L+H P   +  + P      LF+DI +L   R+ E+D + + L    
Sbjct: 10  VSVNTEIGRLRKLLIHSPDSGLGKVVPSKAQDWLFEDIVHLDTIRKGEYDYYIKLL---- 65

Query: 66  VEVLYLEVLAAEAIETSDEVKQQFISEFIDEAGVESE--------RLKEALIEYFNSFSD 117
             + +L+          D++K + +S+   EA   +            +++IE  N  S+
Sbjct: 66  --MYFLD---------PDKIKGK-LSDIDSEASNRNFYKPNHPDFHNSKSVIEIQNLLSE 113

Query: 118 ---NKAMVDKMMAGVRKEELKDYHRESLYD-----QVNNVY--------PFVCDPMPNLY 161
              N+++  K++A V   E   Y  +         Q+  ++          +  P+PNL 
Sbjct: 114 MLENESIRKKLVAAVCAIEGCTYKVQLALTNTDPKQLAEIFISGTLADDTMIFAPIPNLI 173

Query: 162 FTRDPFATIGHGITLNHMRTDTRNRETIFAKYIFRHHPRFEG--------KDIPFWFNRN 213
           FTRD   TI + I LN      R RET+  +YIF +HP FE          D+   F R 
Sbjct: 174 FTRDVGTTINNHILLNKPAKKARVRETLLMRYIFFNHPLFEDYRNNILEIPDVTMHFLRP 233

Query: 214 D-----KTSLEGGDELILSKEILAVGISQRTDSASVEKLAKKLLYYPDTSFKTVLAFKIP 268
                  T+LEGGD +++S   + +G S+RT      + A KLL+  D   K V   KIP
Sbjct: 234 GDEPAFSTTLEGGDVMMVSPNHVLIGCSERTSEHGANE-AIKLLFDQDVVEK-VTVVKIP 291

Query: 269 VSRAFMHLDTVFTQVDYDKFTV-----HPGIVGPLEVYALTKDPENDGQLLVTEEVD--- 320
             R +MHLDTVFTQV  + + +     H     P E     K+PE   +L  T  +    
Sbjct: 292 SKRDYMHLDTVFTQVKRNTWVILNSIAHSPKYNPYEPINFLKEPE---KLEATTAIQFTK 348

Query: 321 ----------TLENILKKYLDRDIK------LIKCGGGDEIIAAREQWNDGSNTLAIAPG 364
                      +E +L      D+K      +I  G       +REQW D  N LAI  G
Sbjct: 349 ANPSEPKHFKHVEALLNDISQNDLKSTEPTQIIYSGNNQFPYDSREQWTDSCNLLAIKDG 408

Query: 365 EVVVYSRNYVTNEILEKEGI------------------------KLHVIPSSELSRGRGG 400
            V+ Y RN  T E  +  G                          L ++PS+ELSR RGG
Sbjct: 409 VVLGYDRNDKTVEAFKTAGFDVVDVKDLIQDLESGKVNVETITDTLILMPSAELSRARGG 468

Query: 401 PRCMSMPLIREDL 413
             CMS+P++R+++
Sbjct: 469 FHCMSLPILRDNI 481


Lambda     K      H
   0.319    0.138    0.396 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 478
Number of extensions: 32
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 413
Length of database: 481
Length adjustment: 32
Effective length of query: 381
Effective length of database: 449
Effective search space:   171069
Effective search space used:   171069
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources