Alignments for a candidate for aruI in Pedobacter sp. GW460-11-11-14-LB5

GapMind for catabolism of small carbon sources

Alignments for a candidate for aruI in Pedobacter sp. GW460-11-11-14-LB5

Align Probable 2-ketoarginine decarboxylase AruI; 2-oxo-5-guanidinopentanoate decarboxylase; 5-guanidino-2-oxopentanoate decarboxylase; EC 4.1.1.75 (characterized)
to candidate CA265_RS15805 CA265_RS15805 acetolactate synthase, large subunit, biosynthetic type

Query= SwissProt::Q9HUI8
         (559 letters)



>FitnessBrowser__Pedo557:CA265_RS15805
          Length = 583

 Score =  196 bits (497), Expect = 3e-54
 Identities = 164/550 (29%), Positives = 253/550 (46%), Gaps = 27/550 (4%)

Query: 25  QPQKTL---TAGQALVRLLANYGVDTVFGIPGVHTLELYRGLP--GSGIRHVLTRHEQGA 79
           +  KTL   T  Q L+  L   GV T+FG PG   + +Y  L      + H+L RHEQG 
Sbjct: 16  ETSKTLFKGTGSQVLLNGLIEEGVTTIFGYPGGAIMPIYDALYDYADKLEHILVRHEQGG 75

Query: 80  GFMADGYARVSGKPGVCFVITGPGVTNVATAIGQAYADSVPLLVISSVNHSASLGKGWGC 139
              A G+AR SG+ GV F  +GPG TN+ T +  A  DS PL+ I+    +  LG     
Sbjct: 76  IHAAQGFARASGEVGVVFATSGPGATNLVTGLADAQIDSTPLVCITGQVFAHLLGT--DA 133

Query: 140 LHETQDQRAMTAPITAFSALALSPEQLPELIARAYAVFDSERPRPVHISI----PLDVLA 195
             ET D   +T P+T ++      +++ E++A+A+ +  S RP PV I I     L +  
Sbjct: 134 FQET-DVINITTPVTKWNYQVTDAKEIQEVLAKAFYIAKSGRPGPVLIDITKNAQLQLEE 192

Query: 196 APVAHDWSAAVARRPGRGVPCSEALRAAAERLAAARRPMLIAGGGAL--AAGEALAALSE 253
            P     +   + RP   V   E +  AAE + +A++P ++ G G +  +A E   A   
Sbjct: 193 FPEYVKCNHIRSYRPKPKVRI-EYIEQAAELINSAKKPFVLFGQGVILGSAEEEFKAFIN 251

Query: 254 RLAAPLFTSVAGKGLLPPDAPLNAG-ASLCVAPGWEMIA-EADLVLAVGTEMADTDFWR- 310
           +   P   ++ G+G +P   PLN G   +    G  ++  EAD+++A+G    D    R 
Sbjct: 252 KTGIPAAWTIMGEGAIPTSHPLNVGMLGMHGNYGPNVLTNEADVIIAIGMRFDDRVTGRL 311

Query: 311 ERLPLSGELIRVDIDPRKFNDFYPSAVALRGDARQTLEALLVRLPQEARDSAPAAARVAR 370
           ++      ++ +DIDP + +    + V + GD ++TL  LL  L  E +     A     
Sbjct: 312 DKYAKQARVVHLDIDPAEIDKNVKAEVGVWGDCKETL-PLLTNLVNENKHEDWLAKFRQY 370

Query: 371 LRAEIRAAHAPL------QALHQAILDRIAAALPADAFVSTDMTQLAYTGNYAFASRAPR 424
            + EI     P       +     +L  I      DA + TD+ Q              R
Sbjct: 371 NQEEIDQVITPELYPTGDEMTMGEVLRNINEICGGDAVIVTDVGQHQMVACRYAKFNNTR 430

Query: 425 SWLHPTGYGTLGYGLPAGIGAKLGAPQRPGLVLVGDGGFLYTAQELATASEELDSPLVVL 484
           S +   G GT+G+GLPA IGAK GAP +  + ++GDGGF  T QEL T   +  + + +L
Sbjct: 431 SNITSGGLGTMGFGLPAAIGAKYGAPDKTVIAIIGDGGFQMTPQELGTIM-QFGAAVKIL 489

Query: 485 LWNNDALGQIRD-DMLGLDIEPVGVLPRNPDFALLGRAYGCAVRQPQDLDELERDLRAGF 543
           + NN  LG +R    L  D     V   +PDF  L ++Y     +  +   L + L    
Sbjct: 490 ILNNRFLGMVRQWQQLFHDKRYSFVNITSPDFVALAKSYYIEASKVDERANLRQALETMI 549

Query: 544 GQSGVTLIEL 553
              G  L+E+
Sbjct: 550 NHEGSYLLEV 559


Lambda     K      H
   0.321    0.136    0.412 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 643
Number of extensions: 41
Number of successful extensions: 7
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 559
Length of database: 583
Length adjustment: 36
Effective length of query: 523
Effective length of database: 547
Effective search space:   286081
Effective search space used:   286081
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources