Align β-glucosidase (H1_GH3) (EC 3.2.1.21) (characterized)
to candidate CA265_RS07180 CA265_RS07180 glycosyl hydrolase
Query= CAZy::AEW47970.1 (758 letters) >FitnessBrowser__Pedo557:CA265_RS07180 Length = 739 Score = 758 bits (1958), Expect = 0.0 Identities = 403/762 (52%), Positives = 525/762 (68%), Gaps = 27/762 (3%) Query: 1 MRKKVIVMLLLPVMICITSRSMLAGEKPDIRTKVDALLSEMTLDEKIGQLNQYTSRWEMT 60 MRK + + L+L V S S +A +K I ++D++L MTLDEKIGQLNQYT + T Sbjct: 1 MRKYLPLALILGV-----SLSAVAQDKK-IEKRIDSVLKLMTLDEKIGQLNQYTGDRDAT 54 Query: 61 GPAPQGKGEQELLEMIRKGQVGSMLNVNGAIATRNAQELAVKNSRLGIPLIFGYDVIHGY 120 GP + L+ IR G++GSMLNV GA TR QE+A++ SR+ IPLIFG DVIHGY Sbjct: 55 GPITNNPNK---LKDIRDGKLGSMLNVRGAKETREVQEVAMQ-SRMKIPLIFGLDVIHGY 110 Query: 121 KTMFPIPLATAASWDPSAAELSARTAATETAASGVHWTFAPMVDIARDARWGRIMEGAGE 180 + FPIPLA AASWD + E +AR AA ETAASGVHWTFAPMVDIARD RWGR+MEGAGE Sbjct: 111 RVTFPIPLAEAASWDLAEMENAARVAARETAASGVHWTFAPMVDIARDPRWGRVMEGAGE 170 Query: 181 DPYLGAQMAAAQVKGFQGNDLSAENTIAACAKHFAAYGFAEAGRDYNTVEITENTLRNVV 240 D YLG+++A A+VKGFQG L + I ACAKHFAAYG A AGRDYN V+++E+TL Sbjct: 171 DTYLGSKIAFARVKGFQGKGLGNLDAIMACAKHFAAYGAAIAGRDYNAVDMSEHTLWETY 230 Query: 241 LPPFKACADAGVATFMNAFNEIGGVTATANKHLVRDILKGEWGFSGYVVSDWNSIGEIYE 300 LPPFKA DAG ATFMN+FN + G+ AT N +L RDILKG+W + G+VVSDW SIGE+ Sbjct: 231 LPPFKAAVDAGAATFMNSFNTLNGIPATGNAYLQRDILKGQWKYQGFVVSDWGSIGEMIA 290 Query: 301 HGMTPDKKEAAFLAIKAGSDMDMEGNAYIAHLKELVEEGRVDESMIDDAVRRILTLKFEL 360 HG DK++AA LA+KAGSDMDME +Y+ L +L+ + +V ++IDDAVRRIL KFEL Sbjct: 291 HGYAKDKEQAAELALKAGSDMDMESRSYLPSLAKLLADKKVPVALIDDAVRRILRKKFEL 350 Query: 361 GLFDDPFRYSDPGK-EKILLSEEHLKAARDVAKKSIVLLKNEKQLLPLKKSGQKIALIGD 419 GLFDDP+R+S+ + EK L S E+ KA+ +A+KSIVLLKNE Q+LPL K +KIA+IG Sbjct: 351 GLFDDPYRFSNESRQEKELNSAENRKASLKMAEKSIVLLKNENQVLPLSKHLKKIAVIGP 410 Query: 420 LADDKDSPLGSWRAQAVAGSAVSLLDGMKNAIQDQRSLTFEQGPVFVTSTPQFTQHLQFN 479 L + G W + VSL G+KN + +Q L + +G + Sbjct: 411 LGKSEKDMQGFWSIAWEKDNLVSLYQGLKNKVGNQTELLYAKG-------------CEVA 457 Query: 480 EKDLTGIDQAVELAEKSDVVVLALGENCFQTGEGRSQTEIGLKGVQQQLLEAVYAANKNM 539 + G +AV +A+++DVV++A+GE +GE +S+ I + GVQ+ L++A+ A K + Sbjct: 458 DSSKAGFAEAVAVAKQADVVIMAVGETLDMSGEAKSKANIHMPGVQEDLIKAIQATGKPV 517 Query: 540 VVVLMNGRPLVIDWMAERVPAIVEAWHLGSEAGNAIADVLFGDYNPSGKLPVSFPRSVGQ 599 VV++M GRPL+ +W A+ VPAI+ W LGSEAGNA+A+VL+GDYNP+GKLP++FPR GQ Sbjct: 518 VVLIMAGRPLIFNWTADHVPAIMNTWWLGSEAGNAMANVLYGDYNPAGKLPMTFPRDEGQ 577 Query: 600 CPIYYNHKNTGRPI--DTGTVFWSHYTDQSNEPLFPFGYGLSYTTFEYADLKLSSSEIRP 657 P+YYN+ +TGRP D T + S Y D N P F FGYGLSYTTF+Y DL LS ++I+ Sbjct: 578 IPLYYNYLSTGRPAKNDKDTRYRSAYLDMPNSPKFAFGYGLSYTTFDYTDLTLSKTQIKQ 637 Query: 658 GEKLKISVNLKNTGKLSGAEVVQLYIRDLYGSVTRPVKELKGFKKISLNPGESRVVEFEI 717 EK+ +S LKNTGK +G EVVQLY++D + SV RPVKELK F+K+ L GE + + F I Sbjct: 638 DEKITVSFTLKNTGKYAGEEVVQLYLQDKFASVVRPVKELKDFQKVMLTAGERKTITFTI 697 Query: 718 SVRDLAFYTADGEWKAEPGHFHLWVGTNSNE-GLKGGFSLKN 758 L+FY +W AE G F L +GT SN L+ F+L N Sbjct: 698 DKEKLSFYNQQLKWAAEAGDFKLMIGTASNNIKLEKDFTLLN 739 Lambda K H 0.317 0.134 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1454 Number of extensions: 59 Number of successful extensions: 6 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 758 Length of database: 739 Length adjustment: 40 Effective length of query: 718 Effective length of database: 699 Effective search space: 501882 Effective search space used: 501882 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 55 (25.8 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory