Align Aconitate hydratase (EC 4.2.1.3) (characterized)
to candidate CA265_RS16400 CA265_RS16400 bifunctional aconitate hydratase 2/2-methylisocitrate dehydratase
Query= reanno::Dino:3608667 (930 letters) >FitnessBrowser__Pedo557:CA265_RS16400 Length = 921 Score = 1420 bits (3676), Expect = 0.0 Identities = 709/918 (77%), Positives = 807/918 (87%), Gaps = 3/918 (0%) Query: 1 MSLYTAYLEEIAARKEQGLQPKPIDDAALTSEIIAQIKDPAHEHRADSLQFFIYNTLPGT 60 MS+Y Y++EI RK QGL PKPID A L SEII QIK+ + +R +++ FFIYNTLPGT Sbjct: 1 MSIYNDYIQEIEDRKAQGLHPKPIDGAELLSEIITQIKNVNNINREEAVNFFIYNTLPGT 60 Query: 61 TSAAGAKAQFLKEIILGESVVAEITPDFAFELLSHMRGGPSVEVLLDIALGDDASLAAQA 120 T+AA KAQFLKEIIL E +VAEITPDFAFELLSHM+GGPS++VLLDIAL D+ A +A Sbjct: 61 TAAAAVKAQFLKEIILAEVIVAEITPDFAFELLSHMKGGPSIKVLLDIALADNGDKAKKA 120 Query: 121 AEVLKTQVFLYEADTDRLKAAHEAGNAVATGILQSYARAEFFTTLPEIEDEIEVVTYIAA 180 A+VLKTQV+LY+ADTDRLK A+ GN +A IL+SYA+AEFFT LPE+ +EI+VVT+IA Sbjct: 121 ADVLKTQVYLYDADTDRLKDAYNNGNEIAKEILESYAKAEFFTKLPEVAEEIKVVTFIAG 180 Query: 181 EGDISTDLLSPGNQAHSRSDRELHGKCMISEAAQKEIEALKLQHPGKRVMLIAEKGTMGV 240 GDISTDLLSPGNQAHSRSDRELHGKCMI+ AQ+EI+AL+ QHP K VML+AEKGTMGV Sbjct: 181 IGDISTDLLSPGNQAHSRSDRELHGKCMITPEAQEEIKALQAQHPDKSVMLVAEKGTMGV 240 Query: 241 GSSRMSGVNNVALWTGKQASPYVPFVNIAPVVAGTNGISPIFMTTVGVTGGIGIDLKNWV 300 GSSRMSGVNNVALWTGK++SPYVPFVNIAP+V GTNGISPIF+TTV VTGGIGIDL+NWV Sbjct: 241 GSSRMSGVNNVALWTGKRSSPYVPFVNIAPIVGGTNGISPIFLTTVDVTGGIGIDLQNWV 300 Query: 301 KKVDGDGNPILNNDGNPILEQKYSVDTGTVLKIDTKARKLMSADGGEELADVSSAFSPQA 360 KK D +GN I N PILEQ++SV+TGTVL I+TK +KL + G +EL D+S A +PQ Sbjct: 301 KKTDENGNVIRNEKDEPILEQRFSVETGTVLTINTKTKKLYN--GEQELIDISKALTPQK 358 Query: 361 VEFMKAGGSYAVVFGKKLQTLAAETLGVEPTPVFAPAKEISHEGQGLTAVEKIFNANARG 420 +EFMKAGGSYA++FGKK+QT AA+TLG+E + VFAPAKE+S+EGQGLTAVEKIFN NA G Sbjct: 359 MEFMKAGGSYAIIFGKKIQTFAAKTLGIEASSVFAPAKEVSYEGQGLTAVEKIFNRNAVG 418 Query: 421 VTPGKVLHAGSDVRVQVNIVGSQDTTGLMTSQELEAMAATVLSPTVDGAYQSGCHTASVW 480 +T GKVLHAGSDVRV+VNIVGSQDTTGLMT+QELEAMAATV+SP VDGAYQSGCHTASVW Sbjct: 419 LTQGKVLHAGSDVRVEVNIVGSQDTTGLMTAQELEAMAATVISPIVDGAYQSGCHTASVW 478 Query: 481 DLKAQANTPRLMAFMHKFGLITARDPKGVYHSMTDVIHKVLNDITVSDWDIIIGGDSHTR 540 D KAQAN P+LM FM+ FG+ITARDPKG YHSMTDVIHKVLNDIT+ +W IIIGGDSHTR Sbjct: 479 DKKAQANIPKLMKFMNDFGVITARDPKGEYHSMTDVIHKVLNDITIDEWAIIIGGDSHTR 538 Query: 541 MSKGVAFGADSGTVALALATGEATMPIPESVKVTFKGKMADHMDFRDVVHATQAQMLAQH 600 MSKGVAFGADSGTVALALATGEA+MPIPESVKVTFKG M +HMDFRDVVHATQ QML Q Sbjct: 539 MSKGVAFGADSGTVALALATGEASMPIPESVKVTFKGLMKEHMDFRDVVHATQLQMLQQF 598 Query: 601 -GDNVFQGRVIEVHIGTLLADQAFTFTDWTAEMKAKASICISNDDTLIESLEIAKQRIQV 659 G+NVFQGR+IEVHIGTLLADQAFTFTDWTAEMKAKASICIS DDTLI+SLEIAK RIQ+ Sbjct: 599 DGENVFQGRIIEVHIGTLLADQAFTFTDWTAEMKAKASICISQDDTLIQSLEIAKNRIQI 658 Query: 660 MIDKGMDNDVQMLAGLIAKANARIAEIRSGEKPALKPDDTARYFAEVVVDLDQIVEPMIA 719 MIDKGMDN Q+L GLI KAN RI EI++G KPAL PDD A+Y+AEVV+DLD I EPMIA Sbjct: 659 MIDKGMDNHNQVLQGLINKANKRIEEIKTGIKPALMPDDNAKYYAEVVIDLDLIEEPMIA 718 Query: 720 DPDVHNADVSKRYTHDTIRPISYYGAEKKIDLGFVGSCMVHKGDVKIVAQMLRNLEKANG 779 DPDV+NADVSKRYTHDTIR +++YG +KK+DLGFVGSCMVHK D+KIV+QML+N+E G Sbjct: 719 DPDVNNADVSKRYTHDTIRDLTFYGGDKKVDLGFVGSCMVHKDDLKIVSQMLKNVETQTG 778 Query: 780 EVKFKAPLVLAAPTYNIIDELKEEGDWDVLQKYAGFEFDDSAPKEKARTEYENILYLERP 839 +V+FKAPLV+AAPTYNIIDELK EGDW+ LQKY+GFEF D+ PK ARTEYENI+YLERP Sbjct: 779 KVEFKAPLVVAAPTYNIIDELKAEGDWEYLQKYSGFEFSDALPKSAARTEYENIMYLERP 838 Query: 840 GCNLCMGNQEKAAKGDTVLATSTRLFQGRVVADSETKKGESLLGSTPVVVLSAILGRTPT 899 GCNLCMGNQEKAAKGDTV+ATSTRLFQGRVV D + KKGESLL STPVVVLSAILGR P+ Sbjct: 839 GCNLCMGNQEKAAKGDTVMATSTRLFQGRVVEDKDGKKGESLLASTPVVVLSAILGRIPS 898 Query: 900 VEEYKTAVEGINLTKFAP 917 +EEYK AVEGINLTKF P Sbjct: 899 IEEYKVAVEGINLTKFTP 916 Lambda K H 0.316 0.132 0.374 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 2223 Number of extensions: 83 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 930 Length of database: 921 Length adjustment: 43 Effective length of query: 887 Effective length of database: 878 Effective search space: 778786 Effective search space used: 778786 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 57 (26.6 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory