Alignments for a candidate for deoD in Pedobacter sp. GW460-11-11-14-LB5

GapMind for catabolism of small carbon sources

Alignments for a candidate for deoD in Pedobacter sp. GW460-11-11-14-LB5

Align Purine nucleoside phosphorylase 1; PNP 1; Inosine phosphorylase; Inosine-guanosine phosphorylase; Purine nucleoside phosphorylase I; PNP I; Pu-NPase I; EC 2.4.2.1 (characterized)
to candidate CA265_RS16505 CA265_RS16505 purine-nucleoside phosphorylase

Query= SwissProt::P77834
         (274 letters)



>FitnessBrowser__Pedo557:CA265_RS16505
          Length = 271

 Score =  259 bits (661), Expect = 6e-74
 Identities = 129/268 (48%), Positives = 180/268 (67%), Gaps = 1/268 (0%)

Query: 5   AIEQAAQFLKEKFPT-SPQIGLILGSGLGVLADEIEQAIKIPYSDIPNFPVSTVEGHAGQ 63
           AIE+  +++K K     P+IG+ILG+GLG L  EIE   ++ YS+IPNFP+ST+E H+G+
Sbjct: 4   AIEETVEYIKRKTENFKPEIGIILGTGLGGLVKEIEVEHQLMYSNIPNFPISTLEFHSGK 63

Query: 64  LVYGQLEGATVVVMQGRFHYYEGYSFDKVTFPVRVMKALGVEQLIVTNAAGGVNESFEPG 123
           L++G L G  ++ MQGR HYYEGYS  ++TFPVRVMK LG+E LIV+NAAG +N  F+ G
Sbjct: 64  LIFGTLNGKKIIAMQGRLHYYEGYSMQQITFPVRVMKGLGIENLIVSNAAGSLNPEFKKG 123

Query: 124 DLMIISDHINNMGGNPLIGPNDSALGVRFPDMSEAYSKRLRQLAKDVANDIGLRVREGVY 183
           DLMII+DHIN    NPL G  +S LG RFPDMS+ Y + +   A ++A ++ +   +GVY
Sbjct: 124 DLMIIADHINLQPDNPLRGLIESELGPRFPDMSQPYKRDIIAKALEIAKNVDINCHKGVY 183

Query: 184 VANTGPAYETPAEIRMIRVMGGDAVGMSTVPEVIVARHAGMEVLGISCISNMAAGILDQP 243
           VA +GP  ET AE + +R++G DAVGMSTVPEVIVA HAG+ V  IS +++       QP
Sbjct: 184 VAVSGPNLETKAEYKYLRLIGADAVGMSTVPEVIVANHAGLPVFAISVLTDEGFPEDLQP 243

Query: 244 LTHDEVIETTEKVKADFLRFVKAIVRNM 271
              DE++    K +      +  ++  +
Sbjct: 244 FNLDEILAAAAKAEPKMTEILTRLISEL 271


Lambda     K      H
   0.319    0.137    0.388 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 227
Number of extensions: 8
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 274
Length of database: 271
Length adjustment: 25
Effective length of query: 249
Effective length of database: 246
Effective search space:    61254
Effective search space used:    61254
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 47 (22.7 bits)

Align candidate CA265_RS16505 CA265_RS16505 (purine-nucleoside phosphorylase)
to HMM TIGR01700 (purine nucleoside phosphorylase I, inosine and guanosine-specific (EC 2.4.2.1))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.carbon/TIGR01700.hmm
# target sequence database:        /tmp/gapView.9904.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR01700  [M=249]
Accession:   TIGR01700
Description: PNPH: purine nucleoside phosphorylase I, inosine and guanosine-specific
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                  Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                  -----------
   7.4e-102  326.0   0.1   8.5e-102  325.8   0.1    1.0  1  lcl|FitnessBrowser__Pedo557:CA265_RS16505  CA265_RS16505 purine-nucleoside 


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__Pedo557:CA265_RS16505  CA265_RS16505 purine-nucleoside phosphorylase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  325.8   0.1  8.5e-102  8.5e-102       1     248 [.      22     268 ..      22     269 .. 0.97

  Alignments for each domain:
  == domain 1  score: 325.8 bits;  conditional E-value: 8.5e-102
                                  TIGR01700   1 diaiilGsGlGelaekvedavildyseiPefpestveGhkGklvfGklegkkvvilqGrfhlyegydl 68 
                                                +i+iilG+GlG l++++e +  l ys+iP+fp st e h+Gkl+fG+l+gkk++++qGr+h+yegy++
  lcl|FitnessBrowser__Pedo557:CA265_RS16505  22 EIGIILGTGLGGLVKEIEVEHQLMYSNIPNFPISTLEFHSGKLIFGTLNGKKIIAMQGRLHYYEGYSM 89 
                                                589***************************************************************** PP

                                  TIGR01700  69 akvtfPvrvlkllGvealvvtnaaGgintefkvGdlmlikdhinllalnPliGpneekfGarfpdmsd 136
                                                +++tfPvrv+k lG+e+l+v+naaG++n+efk+Gdlm+i dhinl + nPl+G  e+++G+rfpdms+
  lcl|FitnessBrowser__Pedo557:CA265_RS16505  90 QQITFPVRVMKGLGIENLIVSNAAGSLNPEFKKGDLMIIADHINLQPDNPLRGLIESELGPRFPDMSQ 157
                                                ******************************************************************** PP

                                  TIGR01700 137 aydkelrqkakeiakelditlkeGvyvavtGPsyetpaevrllkklGadavGmstvpevivarhcGlr 204
                                                 y +++++ka eiak++di+ ++Gvyvav+GP+ et ae++ l+ +GadavGmstvpeviva+h+Gl 
  lcl|FitnessBrowser__Pedo557:CA265_RS16505 158 PYKRDIIAKALEIAKNVDINCHKGVYVAVSGPNLETKAEYKYLRLIGADAVGMSTVPEVIVANHAGLP 225
                                                ******************************************************************** PP

                                  TIGR01700 205 vlglslitnkaagildaelsdheevlevakkakekleklvsalv 248
                                                v+++s++t+++    d +  + +e+l +a ka+ k+++++++l+
  lcl|FitnessBrowser__Pedo557:CA265_RS16505 226 VFAISVLTDEGF-PEDLQPFNLDEILAAAAKAEPKMTEILTRLI 268
                                                **********99.34444448********************998 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (249 nodes)
Target sequences:                          1  (271 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.00u 0.01s 00:00:00.01 Elapsed: 00:00:00.00
# Mc/sec: 7.53
//
[ok]

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources