Alignments for a candidate for lacZ in Pedobacter sp. GW460-11-11-14-LB5

GapMind for catabolism of small carbon sources

Alignments for a candidate for lacZ in Pedobacter sp. GW460-11-11-14-LB5

Align β-galactosidase (Bga;NixL;XCC2895) (EC 3.2.1.23) (characterized)
to candidate CA265_RS06080 CA265_RS06080 beta-galactosidase

Query= CAZy::AAM42167.1
         (613 letters)



>FitnessBrowser__Pedo557:CA265_RS06080
          Length = 611

 Score =  483 bits (1243), Expect = e-140
 Identities = 251/604 (41%), Positives = 365/604 (60%), Gaps = 10/604 (1%)

Query: 15  IALPITATAASDDQWPTFATQGTQFVRDGKPYQVLSGAIHFQRIPRTYWKDRLQKARALG 74
           +A+  + TA       TF    + F+ D KP+Q++SG +H+ R+P+  W+ R++ A+A+G
Sbjct: 9   VAMCFSLTAFGQQAKHTFKLGESDFLLDDKPFQIISGELHYPRVPKEAWRARMKMAKAMG 68

Query: 75  LNTVETYVFWNLVEPQQGQFDFNANNDVAAFVREAAAQGLNVILRPGPYACAEWEAGGYP 134
           LNT+ TYVFWNL EPQ+G+FDF+ NN++A FV+ A  +GL VILRP PY CAEWE GGYP
Sbjct: 69  LNTIGTYVFWNLHEPQKGKFDFSGNNNIAEFVKIAQQEGLWVILRPSPYVCAEWEFGGYP 128

Query: 135 AWLFGKDNIRIRSRDPRFLAASQSYLDAVAQQVRPLLNHNGGPIIAVQVENEYGSYDDDH 194
            WL  +  + +RS + ++L   + Y+  V +Q+  L  ++GG II VQVENEYGSY  D 
Sbjct: 129 YWLQNEKGLVVRSTEKQYLDEYKKYILEVGKQLASLQINHGGNIIMVQVENEYGSYAADK 188

Query: 195 AYMADNRAMFVKAGFDKALLFTSDGADMLANGTLPGTLAVVNFAPGEAKSAFDKLIKFQP 254
            Y+  NR MF++AGFD  LL+T D A  + +G L G L  VN     AK    +L+    
Sbjct: 189 NYLDINRKMFIEAGFD-GLLYTCDPAADVKDGHLAGLLPAVNGIDDPAK--IRQLVNENH 245

Query: 255 D--QPRMVGEYWAGWFDHWGTPHASTNAKQQTEELEWILRQGHSANLYMFIGGTSFGFMN 312
           D   P  + E++  WFD WGT H +  A Q T+ L+ +L  G S N+YMF GGT+ GFMN
Sbjct: 246 DGKGPYFIAEWYPAWFDWWGTAHHTVPAVQYTKRLDSVLAAGISINMYMFHGGTTRGFMN 305

Query: 313 GANFQGNPSDHYAPQTTSYDYDAILDEAGRPTPKFALMRDVITR--VTGVQPPALPAPIA 370
           GAN++      Y PQT+SYDYDA L+EAG  T KF   R VI +    G   P +PA   
Sbjct: 306 GANYK--DISPYEPQTSSYDYDAPLNEAGNVTEKFRAFRSVIEKHLPAGQSLPPIPAAKP 363

Query: 371 MAALKDAPLRESASLWDNLPAPIAIDTPQPMEHFGQDYGYILYRTTVTGPRKESLYLGEV 430
             A+    L    +L++ LP  +   TP   E   QDYGY+LYRTT+ G +K  L L ++
Sbjct: 364 SIAIAPFKLTSKVTLFNALPKAVKNITPLTFEDLKQDYGYVLYRTTIQGGKKGQLQLKQL 423

Query: 431 RDVARVYVDQKPVGSVERRLQQVATEVDIPAGQHTLDVLVENSGRINYGPRMADGRAGLV 490
           RD A ++++QK  G ++RRL Q    +D+PAG+  LD+ VEN GR+N+G  + + + G+ 
Sbjct: 424 RDYALIFINQKRAGILDRRLNQNTLSLDLPAGEVQLDIFVENMGRVNFGKYLLENKKGIT 483

Query: 491 DPVLLDNQQLTNWQAFPLPMRSPD-SIRGWTRNTVEGPAFHRGNLRIGTPADTYLDMRAF 549
           + V  +  ++ NW  +  P  +   +I   +++   GP   +G+ +I T ADTYLDM  +
Sbjct: 484 EQVTFNGAEVKNWSMYGFPFNNDRMTITSPSKSNGNGPTLQKGSFQIATVADTYLDMTDW 543

Query: 550 GKGIAWANGVNLGRHWNIGPQRALYFPAPFQRKGDNTVVVFDLDSTAKPSVRGLQQQVWI 609
           GKG+ W NG NLG++W IGPQ+ LY P  + +KG N + V +L    +  ++ + Q +  
Sbjct: 544 GKGVVWVNGHNLGKYWAIGPQQTLYVPQEWLKKGKNDITVLELLKPDQNILKAIDQPILN 603

Query: 610 TPKE 613
           T K+
Sbjct: 604 TLKK 607


Lambda     K      H
   0.319    0.136    0.425 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1073
Number of extensions: 51
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 613
Length of database: 611
Length adjustment: 37
Effective length of query: 576
Effective length of database: 574
Effective search space:   330624
Effective search space used:   330624
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources